An effective electrochemical biosensing platform for the detection of reduced glutathione

Abstract

A biosensor was developed using glutathione peroxidase (GSH-Px) as the enzyme. Firstly, platinum (Pt) nanoparticles were deposited onto a glassy carbon paste electrode (GCPE), and then GSH-Px was immobilized by means of gelatin that was then crosslinked with glutaraldehyde. The measurement was based on the electrochemical oxidation of GSH to its disulfide form in the presence of hydrogen peroxide. The linear range was found to be between 10 and 250 μM, with a correlation coefficient of R² = 0.9968. The R.S.D value for 25 μM GSH (n = 6) was calculated as 2.92%. Finally, the proposed biosensor was used to analyze GSH in a synthetically prepared plasma sample, and a promising recovery value was obtained.

Keywords::

• biosensor
• glassy carbon paste electrode
• glutathione
• glutathione peroxidase
• Pt nanoparticles

Acknowledgements

This work is supported by The Scientific and Technical Research Council of Turkiye (TUBITAK) (project no. 109T885). M.C. and F.N.E are gratefully acknowledged the grant from Ege University, Project number 2011 FEN 088.

Declaration of interest

The authors report no declarations of interest. The authors alone are responsible for the content and writing of the paper.