Antioxidant effects of vitamin C on hemoglobin-based oxygen carriers derived from human cord blood

Abstract

Objective To investigated the antioxidant protection effects of vitamin C (Vc) on the hemoglobin-based oxygen carriers (HBOCs) solution. Methods Three influential variables were studied including the molar ratio of Vc and hemoglobin, pH and temperature. The HBOCs molecules were characterized through spectral analysis, circular dichroism spectra, the oxygen binding curves and the molecular weights. Results The optimized conditions were molar ratios of above 3, pH of 7.5–8.0 and temperature of 4 °C. HBOCs characterization showed no differences before and after Vc addition. Conclusion Antioxidant effects in HBOCs solution could be presented by Vc, indicating the potential application of Vc in HBOCs development.

Keywords:

• antioxidant effects
• hemoglobin-based oxygen carriers
• methemoglobin
• vitamin C

Declaration of interest

This study was financially supported by the Sichuan International Cooperation and Exchange Research Program (No. 2014HH0065), Sichuan Science and Technology Support Program (No. 2014SZ0123), Scientific Research Program of Sichuan Provincial Health and Family Planning Commission (No. 150256).