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Xenobiotica
the fate of foreign compounds in biological systems
Volume 33, 2003 - Issue 6
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Research Article

Verapamil: identification of novel metabolites in cultures of primary human hepatocytes and human urine by LC-MS n and LC-NMR

J. Borlak Departments of Analytical Chemistry, Fraunhofer Institute of Toxicology and Experimental Medicine, Nikolai-Fuchs Str. 1, D-30625 Hannover, Germany
,
M. Walles Departments of Analytical Chemistry, Fraunhofer Institute of Toxicology and Experimental Medicine, Nikolai-Fuchs Str. 1, D-30625 Hannover, Germany
,
M. Elend Departments of Analytical Chemistry, Fraunhofer Institute of Toxicology and Experimental Medicine, Nikolai-Fuchs Str. 1, D-30625 Hannover, Germany
,
T. Thum Departments of Drug Research and Medical Biotechnology, Fraunhofer Institute of Toxicology and Experimental Medicine, Nikolai-Fuchs Str. 1, D-30625 Hannover, Germany
,
A. Preiss Departments of Analytical Chemistry, Fraunhofer Institute of Toxicology and Experimental Medicine, Nikolai-Fuchs Str. 1, D-30625 Hannover, Germany
&
K. Levsen Departments of Analytical Chemistry, Fraunhofer Institute of Toxicology and Experimental Medicine, Nikolai-Fuchs Str. 1, D-30625 Hannover, Germany
Pages 655-676 | Published online: 22 Sep 2008

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B. Kammerer, H. Scheible, G. Zurek, M. Godejohann, K.-P. Zeller, C. H. Gleiter, W. Albrecht & S. Laufer. (2007) In vitro metabolite identification of ML3403, a 4-pyridinylimidazole-type p38 MAP kinase inhibitor by LC-Qq-TOF-MS and LC-SPE-cryo-NMR/MS. Xenobiotica 37:3, pages 280-297.
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Articles from other publishers (25)

Shi Qiao, Haishan Xu, Wenfang Zhang, Wei Yang, Danming Guo, Wei Wang, Weizhe Xu, Yongsuo Liu, Guoru Liu, Yujing Cui, Hongyan Yu & Qingyan Li. (2020) Identification of characteristic heroin metabolites in urine based on data-mining technology and multivariate statistics analysis combined with a targeted verification approach for distinguishing heroin abusers. Journal of Chromatography B 1152, pages 122251.
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Jelle Reinen, Martijn Smit & Mira Wenker. (2018) Evaluation of Strategies for the Assessment of Drug–Drug Interactions Involving Cytochrome P450 Enzymes. European Journal of Drug Metabolism and Pharmacokinetics 43:6, pages 737-750.
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Tao Wu, Gaëtan Bour, Sarah Durand, Véronique Lindner, Francine Gossé, Laetitia Zona, Jean-Marie Certoux, Michele Diana, Thomas F. BaumertJacques MarescauxDidier MutterPatrick PessauxEric Robinet. (2015) Use of a Closed Culture System to Improve the Safety of Lentiviral Vector Production. Human Gene Therapy Methods 26:6, pages 197-210.
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Jürgen Borlak, Prafull Kumar Singh & Ina Rittelmeyer. (2015) Regulation of Liver Enriched Transcription Factors in Rat Hepatocytes Cultures on Collagen and EHS Sarcoma Matrices. PLOS ONE 10:4, pages e0124867.
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Tanja Hansen, Anil Chougule & Jürgen Borlak. (2014) Isolation and cultivation of metabolically competent alveolar epithelial cells from A/J mice. Toxicology in Vitro 28:5, pages 812-821.
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Sandra Jahn, Anne Baumann, Jörg Roscher, Katharina Hense, Raniero Zazzeroni & Uwe Karst. (2011) Investigation of the biotransformation pathway of verapamil using electrochemistry/liquid chromatography/mass spectrometry – A comparative study with liver cell microsomes. Journal of Chromatography A 1218:51, pages 9210-9220.
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Antonia F. Stepan, Daniel P. Walker, Jonathan Bauman, David A. Price, Thomas A. Baillie, Amit S. Kalgutkar & Michael D. Aleo. (2011) Structural Alert/Reactive Metabolite Concept as Applied in Medicinal Chemistry to Mitigate the Risk of Idiosyncratic Drug Toxicity: A Perspective Based on the Critical Examination of Trends in the Top 200 Drugs Marketed in the United States. Chemical Research in Toxicology 24:9, pages 1345-1410.
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Zhi-Hua Li, Josef Velisek, Vladimir Zlabek, Roman Grabic, Jana Machova, Jitka Kolarova, Ping Li & Tomas Randak. (2011) Chronic toxicity of verapamil on juvenile rainbow trout (Oncorhynchus mykiss): Effects on morphological indices, hematological parameters and antioxidant responses. Journal of Hazardous Materials 185:2-3, pages 870-880.
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Fraser F. Fleming, Lihua Yao, P. C. Ravikumar, Lee Funk & Brian C. Shook. (2010) Nitrile-Containing Pharmaceuticals: Efficacious Roles of the Nitrile Pharmacophore. Journal of Medicinal Chemistry 53:22, pages 7902-7917.
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Lukáš Chytil, Branislav Štrauch, Josef Cvačka, Věra Marešová, Jiří WidimskýJr.Jr., Robert Holaj & Ondřej Slanař. (2010) Determination of doxazosin and verapamil in human serum by fast LC–MS/MS: Application to document non-compliance of patients. Journal of Chromatography B 878:30, pages 3167-3173.
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Shayne Cox GadMehran F. Moghaddam. 2010. Pharmaceutical Sciences Encyclopedia. Pharmaceutical Sciences Encyclopedia.
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Christoph Trautwein, Klaus Kümmerer & Jörg W. Metzger. (2008) Aerobic biodegradability of the calcium channel antagonist verapamil and identification of a microbial dead-end transformation product studied by LC–MS/MS. Chemosphere 72:3, pages 442-450.
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Que N. Van, Haleem J. Issaq, Qiujie Jiang, Qiaoli Li, Gary M. Muschik, Timothy J. Waybright, Hong Lou, Michael Dean, Jouni Uitto & Timothy D. Veenstra. (2007) Comparison of 1D and 2D NMR Spectroscopy for Metabolic Profiling. Journal of Proteome Research 7:2, pages 630-639.
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Markus Walles, Caroline Gauvin, Pierre-Emmanuel Morin, Rosemarie Panetta & Julie Ducharme. (2007) Comparison of sub-2-μm particle columns for fast metabolite ID. Journal of Separation Science 30:8, pages 1191-1199.
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Karsten Levsen, Hans-Martin Schiebel, Beate Behnke, Reinhard Dötzer, Wolfgang Dreher, Manfred Elend & Herbert Thiele. (2005) Structure elucidation of phase II metabolites by tandem mass spectrometry: an overview. Journal of Chromatography A 1067:1-2, pages 55-72.
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Wayne M Mullett, Markus Walles, Karsten Levsen, Jürgen Borlak & Janusz Pawliszyn. (2004) Multidimensional on-line sample preparation of verapamil and its metabolites by a molecularly imprinted polymer coupled to liquid chromatography–mass spectrometry. Journal of Chromatography B 801:2, pages 297-306.
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M Walles, T Thum, K Levsen & J Borlak. (2003) Metabolism of verapamil: 24 new phase I and phase II metabolites identified in cell cultures of rat hepatocytes by liquid chromatography–tandem mass spectrometry. Journal of Chromatography B 798:2, pages 265-274.
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