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Current Eye Research Volume 31, 2006 - Issue 5
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Original Article

Corneal Epithelial Cultures Generated from Organ-Cultured Limbal Tissue: Factors Influencing Epithelial Cell Growth

Eleonora Zito-Abbad Cornea Bank of EFS-Ile-de-France and Department of Ophthalmology, Centre Hospitalier National d'Ophtalmologie des XV-XX, Paris VI University, 28 rue de Charenton, Paris, 75012, France
,
Vincent M. Borderie Cornea Bank of EFS-Ile-de-France and Department of Ophthalmology, Centre Hospitalier National d'Ophtalmologie des XV-XX, Paris VI University, 28 rue de Charenton, Paris, 75012, France
,
Marie Baudrimont Cornea Bank of EFS-Ile-de-France and Department of Ophthalmology, Centre Hospitalier National d'Ophtalmologie des XV-XX, Paris VI University, 28 rue de Charenton, Paris, 75012, France
,
Tristan Bourcier Cornea Bank of EFS-Ile-de-France and Department of Ophthalmology, Centre Hospitalier National d'Ophtalmologie des XV-XX, Paris VI University, 28 rue de Charenton, Paris, 75012, France
,
Laurent Laroche Cornea Bank of EFS-Ile-de-France and Department of Ophthalmology, Centre Hospitalier National d'Ophtalmologie des XV-XX, Paris VI University, 28 rue de Charenton, Paris, 75012, France
,
Catherine Chapel Radiobiology and Radiopathology Department, C.E.A. BP. 6, Fontenay aux Roses, 92265, France
&
Jean-Louis Uzel Hôpital Civil de Strasbourg, 1, place de l'Hôpital, Strasbourg, 67000, France
 show all
Pages 391-399 | Received 28 Aug 2005, Accepted 09 Mar 2006, Published online: 02 Jul 2009

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Djida Ghoubay-Benallaoua, Florence Pécha, Pablo Goldschmidt, Anne Fialaire-Legendre, Christine Chaumeil, Laurent Laroche & Vincent M. Borderie. (2012) Effects of Isoproterenol and Cholera Toxin on Human Limbal Epithelial Cell Cultures. Current Eye Research 37:7, pages 644-653.
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Otman Sandali, Thomas Gaujoux, Pablo Goldschmidt, Djida Ghoubay-Benallaoua, Laurent Laroche & Vincent M. Borderie. (2012) Infectious Keratitis in Severe Limbal Stem Cell Deficiency: Characteristics and Risk Factors. Ocular Immunology and Inflammation 20:3, pages 182-189.
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Sharon L Mason, Rosalind MK Stewart, Victoria R Kearns, Rachel L Williams & Carl M Sheridan. (2011) Ocular Epithelial Transplantation: Current uses and Future Potential. Regenerative Medicine 6:6, pages 767-782.
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Charles Osei-Bempong, Christin Henein & Sajjad Ahmad. (2009) Culture Conditions for Primary Human Limbal Epithelial Cells. Regenerative Medicine 4:3, pages 461-470.
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Articles from other publishers (15)

Zhen Li, Daniel Böhringer, Tanja Stachon, Mahsa Nastaranpour, Fabian Norbert Fries, Berthold Seitz, Myriam Ulrich, Cristian Munteanu, Achim Langenbucher & Nóra Szentmáry. (2023) Culturing Limbal Epithelial Cells of Long-term Stored Corneal Donors (Organ Culture) In Vitro – A Stepwise Linear Regression Algorithm. Klinische Monatsblätter für Augenheilkunde.
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Alice Rocha Teixeira Netto, Marc Dieter Hrusa, Karl-Ulrich Bartz-Schmidt, Sven Schnichels & José Hurst. (2023) Two Methods for the Isolation and Cultivation of Porcine Primary Corneal Cells. Methods and Protocols 6:3, pages 50.
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Pei-Xi Ying, Min Fu, Chang Huang, Zhi-Hong Li, Qing-Yi Mao, Sheng Fu, Xu-Hui Jia, Yu-Chen Cao, Li-Bing Hong, Li-Yang Cai, Xi Guo, Ru-Bing Liu, Fan-ke Meng & Guo-Guo Yi. (2022) Profile of biological characterizations and clinical application of corneal stem/progenitor cells. World Journal of Stem Cells 14:11, pages 777-797.
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Gaëtan Le-Bel, Pascale Desjardins, Christelle Gross, Sergio Cortez Ghio, Camille Couture, Lucie Germain & Sylvain L. Guérin. (2022) Influence of the Postmortem/Storage Time of Human Corneas on the Properties of Cultured Limbal Epithelial Cells. Cells 11:17, pages 2716.
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O. A. Utheim, L. Pasovic, S. Raeder, J. R. Eidet, I. G. Fostad, A. Sehic, B. Roald, M. F. de la Paz, T. Lyberg, D. A. Dartt & T. P. Utheim. (2019) Effects of explant size on epithelial outgrowth, thickness, stratification, ultrastructure and phenotype of cultured limbal epithelial cells. PLOS ONE 14:3, pages e0212524.
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GAMZE DERELI CAN, ÖZGE EKIN AKDERE, MEHMET EROL CAN & MENEMŞE GÜMÜŞDERELIOĞLU. (2019) A simple and efficient method for cultivation of limbal explant stem cells with clinically safe potential. Cytotherapy 21:1, pages 83-95.
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Yolanda Lorenzo, Kristiane Haug Berg, Amund Ringvold, Goran Petrovski, Morten C. Moe, Andrew Collins & Bjørn Nicolaissen. (2018) Levels of oxidative DNA damage are low in ex vivo engineered human limbal epithelial tissue . Acta Ophthalmologica 96:8, pages 834-840.
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Meeta Pathak, O.K. Olstad, Liv Drolsum, Morten C. Moe, Natalia Smorodinova, Sarka Kalasova, Katerina Jirsova, Bjørn Nicolaissen & Agate Noer. (2016) The effect of culture medium and carrier on explant culture of human limbal epithelium: A comparison of ultrastructure, keratin profile and gene expression. Experimental Eye Research 153, pages 122-132.
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Marina López-Paniagua, Teresa Nieto-Miguel, Ana de la Mata, Marc Dziasko, Sara Galindo, Esther Rey, José M. Herreras, Rosa M. Corrales, Julie T. Daniels & Margarita Calonge. (2016) Comparison of functional limbal epithelial stem cell isolation methods. Experimental Eye Research 146, pages 83-94.
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Catherine Jackson, Peder Aabel, Jon R. Eidet, Edward B. Messelt, Torstein Lyberg, Magnus von Unge & Tor P. Utheim. (2014) Effect of Storage Temperature on Cultured Epidermal Cell Sheets Stored in Xenobiotic-Free Medium. PLoS ONE 9:8, pages e105808.
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Samantha Wilson, Laura Sidney, Siobhán Dunphy, James Rose & Andrew Hopkinson. (2013) Keeping an Eye on Decellularized Corneas: A Review of Methods, Characterization and Applications. Journal of Functional Biomaterials 4:3, pages 114-161.
Crossref
Tor Paaske Utheim, Torstein Lyberg & Sten Ræder. 2013. Corneal Regenerative Medicine. Corneal Regenerative Medicine 103 129 .
V. Borderie, P. Borderie, É. Basli, A. Piquemal, C. De Sousa, P. Goldschmidt, L. Laroche & A. Fialaire. (2010) Cinétique de croissance in vitro des cellules épithéliales cultivées à partir d’explants limbiques humains. Journal Français d'Ophtalmologie 33:7, pages 465-471.
Crossref
Urs Vossmerbaeumer, Sandra Kuehl, Karen Bieback, Harald Klüter & Jost B. Jonas. (2008) Cultivation and differentiation characteristics of human limbal progenitor cells. Tissue and Cell 40:2, pages 83-88.
Crossref
Dan-Ning Hu, Steven A. McCormick, John A. Seedor, David C. Ritterband & Mahendra K. Shah. (2007) Isolation, purification and cultivation of conjunctival melanocytes. Experimental Eye Research 84:4, pages 655-662.
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