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Molecular Membrane Biology Volume 17, 2000 - Issue 1
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Research Article

Amino acid residues N450 and Q449 are critical for the uptake capacity and specificity of UapA, a prototype of a nucleobase-ascorbate transporter family

Meintanis Christos Microbiology Laboratory, Section Botany, Department of Biology, University of Athens, Panepistimioupolis, Athens 15781, Greece
,
Amalia D. Karagouni Microbiology Laboratory, Section Botany, Department of Biology, University of Athens, Panepistimioupolis, Athens 15781, Greece
&
George Diallinas Microbiology Laboratory, Section Botany, Department of Biology, University of Athens, Panepistimioupolis, Athens 15781, GreeceCorrespondence[email protected]
Pages 47-57 | Published online: 09 Jul 2009

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Areti Pantazopoulou & George Diallinas. (2006) The first transmembrane segment (TMS1) of UapA contains determinants necessary for expression in the plasma membrane and purine transport. Molecular Membrane Biology 23:4, pages 337-348.
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Sophia Goudela, Panayiota Karatza, Marina Koukaki, Stathis Frillingos & George Diallinas. (2005) Comparative substrate recognition by bacterial and fungal purine transporters of the NAT/NCS2 family. Molecular Membrane Biology 22:3, pages 263-275.
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Panayiota Karatza & Stathis Frillingos. (2005) Cloning and functional characterization of two bacterial members of the NAT/NCS2 family in Escherichia coli. Molecular Membrane Biology 22:3, pages 251-261.
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S. N. Tavoularis, U. H. Tazebay, G. Diallinas, M. Sideridou, A. Rosa, C. Scazzocchio & V. Sophianopoulou. (2003) Mutational analysis of the major proline transporter (PrnB) of Aspergillus nidulans. Molecular Membrane Biology 20:4, pages 285-297.
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Harry de Koning, George Diallinas. (2000) Nucleobase transporters. Molecular Membrane Biology 17:2, pages 75-94.
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Articles from other publishers (31)

Georgia Sioupouli, George Lambrinidis, Emmanuel Mikros, Sotiris Amillis & George Diallinas. (2017) Cryptic purine transporters in Aspergillus nidulans reveal the role of specific residues in the evolution of specificity in the NCS1 family . Molecular Microbiology 103:2, pages 319-332.
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Emilia Krypotou, Claudio Scazzocchio & George Diallinas. (2015) Functional characterization of NAT/NCS2 proteins of Aspergillus brasiliensis reveals a genuine xanthine–uric acid transporter and an intrinsically misfolded polypeptide. Fungal Genetics and Biology 75, pages 56-63.
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George Diallinas. (2014) Understanding transporter specificity and the discrete appearance of channel-like gating domains in transporters. Frontiers in Pharmacology 5.
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Xiaofeng Cai, Jie Ye, Tixu Hu, Yuyang Zhang, Zhibiao Ye & Hanxia Li. (2013) Genome-wide classification and expression analysis of nucleobase–ascorbate transporter (NAT) gene family in tomato. Plant Growth Regulation 73:1, pages 19-30.
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George Diallinas. (2013) Allopurinol and xanthine use different translocation mechanisms and trajectories in the fungal UapA transporter. Biochimie 95:9, pages 1755-1764.
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Marc Bürzle, Yoshiro Suzuki, Daniel Ackermann, Hiroki Miyazaki, Nobuyo Maeda, Benjamin Clémençon, Robert Burrier & Matthias A. Hediger. (2013) The sodium-dependent ascorbic acid transporter family SLC23. Molecular Aspects of Medicine 34:2-3, pages 436-454.
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Vasiliki Kosti, George Lambrinidis, Vassilios Myrianthopoulos, George Diallinas & Emmanuel Mikros. (2012) Identification of the Substrate Recognition and Transport Pathway in a Eukaryotic Member of the Nucleobase-Ascorbate Transporter (NAT) Family. PLoS ONE 7:7, pages e41939.
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Sotiris Amillis, Vasiliki Kosti, Areti Pantazopoulou, Emmanuel Mikros & George Diallinas. (2011) Mutational Analysis and Modeling Reveal Functionally Critical Residues in Transmembrane Segments 1 and 3 of the UapA Transporter. Journal of Molecular Biology 411:3, pages 567-580.
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James Leung, Mayia Karachaliou, Claudia Alves, George Diallinas & Bernadette Byrne. (2010) Expression and purification of a functional uric acid–xanthine transporter (UapA). Protein Expression and Purification 72:1, pages 139-146.
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Vasiliki Kosti, Ioannis Papageorgiou & George Diallinas. (2010) Dynamic Elements at Both Cytoplasmically and Extracellularly Facing Sides of the UapA Transporter Selectively Control the Accessibility of Substrates to Their Translocation Pathway. Journal of Molecular Biology 397:5, pages 1132-1143.
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Zsuzsanna Hamari, Sotiris Amillis, Christine Drevet, Angeliki Apostolaki, Csaba Vágvölgyi, George Diallinas & Claudio Scazzocchio. (2009) Convergent evolution and orphan genes in the Fur4p-like family and characterization of a general nucleoside transporter in Aspergillus nidulans . Molecular Microbiology 73:1, pages 43-57.
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C. I. Rivas, F. A. Zúñiga, A. Salas-Burgos, L. Mardones, V. Ormazabal & J. C. Vera. (2008) Vitamin C transporters. Journal of Physiology and Biochemistry 64:4, pages 357-375.
Crossref
Ioannis Papageorgiou, Christos Gournas, Anna Vlanti, Sotiris Amillis, Areti Pantazopoulou & George Diallinas. (2008) Specific Interdomain Synergy in the UapA Transporter Determines Its Unique Specificity for Uric Acid among NAT Carriers. Journal of Molecular Biology 382:5, pages 1121-1135.
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Konstantinos Papakostas, Ekaterini Georgopoulou & Stathis Frillingos. (2008) Cysteine-scanning Analysis of Putative Helix XII in the YgfO Xanthine Permease. Journal of Biological Chemistry 283:20, pages 13666-13678.
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Sophia Goudela, Utz Reichard, Sotiris Amillis & George Diallinas. (2008) Characterization and kinetics of the major purine transporters in Aspergillus fumigatus. Fungal Genetics and Biology 45:4, pages 459-472.
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I. Savini, A. Rossi, C. Pierro, L. Avigliano & M. V. Catani. (2007) SVCT1 and SVCT2: key proteins for vitamin C uptake. Amino Acids 34:3, pages 347-355.
Crossref
Christos Gournas, Ioannis Papageorgiou & George Diallinas. (2008) The nucleobase–ascorbate transporter (NAT) family: genomics, evolution, structure–function relationships and physiological role. Molecular BioSystems 4:5, pages 404.
Crossref
Areti Pantazopoulou & George Diallinas. (2007) Fungal nucleobase transporters. FEMS Microbiology Reviews 31:6, pages 657-675.
Crossref
Panagiota Kafasla, Demetra Bouzarelou, Stathis Frillingos & Vicky Sophianopoulou. (2007) The proline permease of Aspergillus nidulans: Functional replacement of the native cysteine residues and properties of a cysteine-less transporter. Fungal Genetics and Biology 44:7, pages 615-626.
Crossref
Areti Pantazopoulou, Njimoh Dieudonné Lemuh, Dimitris G. Hatzinikolaou, Christine Drevet, Gianna Cecchetto, Claudio Scazzocchio & George Diallinas. (2007) Differential physiological and developmental expression of the UapA and AzgA purine transporters in Aspergillus nidulans. Fungal Genetics and Biology 44:7, pages 627-640.
Crossref
Panayiota Karatza, Panayiotis Panos, Ekaterini Georgopoulou & Stathis Frillingos. (2006) Cysteine-scanning Analysis of the Nucleobase-Ascorbate Transporter Signature Motif in YgfO Permease of Escherichia coli. Journal of Biological Chemistry 281:52, pages 39881-39890.
Crossref
Z. Erpapazoglou, P. Kafasla & V. Sophianopoulou. (2006) The product of the SHR3 orthologue of Aspergillus nidulans has restricted range of amino acid transporter targets. Fungal Genetics and Biology 43:4, pages 222-233.
Crossref
Anna Vlanti, Sotiris Amillis, Marina Koukaki & George Diallinas. (2006) A Novel-type Substrate-selectivity Filter and ER-exit Determinants in the UapA Purine Transporter. Journal of Molecular Biology 357:3, pages 808-819.
Crossref
Marina Koukaki, Anna Vlanti, Sophia Goudela, Areti Pantazopoulou, Harris Gioule, Stella Tournaviti & George Diallinas. (2005) The Nucleobase-ascorbate Transporter (NAT) Signature Motif in UapA Defines the Function of the Purine Translocation Pathway. Journal of Molecular Biology 350:3, pages 499-513.
Crossref
Anja Schmidt, Yan-Hua Su, Reinhard Kunze, Susan Warner, Matthew Hewitt, Robert D. Slocum, Uwe Ludewig, Wolf B. Frommer & Marcelo Desimone. (2004) UPS1 and UPS2 from Arabidopsis Mediate High Affinity Transport of Uracil and 5-Fluorouracil. Journal of Biological Chemistry 279:43, pages 44817-44824.
Crossref
Sotiris Amillis, Gianna Cecchetto, Vicky Sophianopoulou, Marina Koukaki, Claudio Scazzocchio & George Diallinas. (2004) Transcription of purine transporter genes is activated during the isotropic growth phase of Aspergillus nidulans conidia. Molecular Microbiology 52:1, pages 205-216.
Crossref
Gianna Cecchetto, Sotiris Amillis, George Diallinas, Claudio Scazzocchio & Christine Drevet. (2004) The AzgA Purine Transporter of Aspergillus nidulans. Journal of Biological Chemistry 279:5, pages 3132-3141.
Crossref
Javier Valdez-Taubas, Laura Harispe, Claudio Scazzocchio, Lisette Gorfinkiel & Alberto L. Rosa. (2004) Ammonium-induced internalisation of UapC, the general purine permease from Aspergillus nidulans. Fungal Genetics and Biology 41:1, pages 42-51.
Crossref
Qi Li & Neil P Schultes. (2002) Arabidopsis thaliana locus At5g62890, a nucleobase-ascorbate transporter family member, is preferentially expressed in carpel transmitting tract and tapetal cells. Plant Science 163:2, pages 233-240.
Crossref
Sotiris Amillis, Marina Koukaki & George Diallinas. (2001) Substitution F569S converts UapA, a specific uric acid-xanthine transporter, into a broad specificity transporter for purine-related solutes. Journal of Molecular Biology 313:4, pages 765-774.
Crossref
Renaud Wagner, Marie-Laure Straub, Jean-Luc Souciet, Serge Potier & Jacky de Montigny. (2001) New Plasmid System To Select for Saccharomyces cerevisiae Purine-Cytosine Permease Affinity Mutants . Journal of Bacteriology 183:14, pages 4386-4388.
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