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Leukemia & Lymphoma Volume 45, 2004 - Issue 2
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Original Article

Immunophenotypic Analysis of Hematogones (B-Lymphocyte Precursors) and Neoplastic Lymphoblasts by 4-Color Flow Cytometry

ROBERT W MCKENNA The University of Texas Southwestern Medical Center, Department of Pathology, 5323 Harry Hines Blvd., Dallas, Texas, 75390-9072, USACorrespondence[email protected]
,
SHERYL L ASPLUND The University of Texas Southwestern Medical Center, Department of Pathology, 5323 Harry Hines Blvd., Dallas, Texas, 75390-9072, USA
&
STEVEN H KROFT The University of Texas Southwestern Medical Center, Department of Pathology, 5323 Harry Hines Blvd., Dallas, Texas, 75390-9072, USA
Pages 277-285 | Received 20 May 2003, Published online: 03 Aug 2009

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Victor Santiago, Aleksandr Lazaryan, Brian McClune, Robert W. McKenna & Elizabeth L. Courville. (2018) Quantification of marrow hematogones following autologous stem cell transplant in adult patients with plasma cell myeloma or diffuse large B-cell lymphoma and correlation with outcome. Leukemia & Lymphoma 59:4, pages 958-966.
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Deborah W. Sevilla, Adriana I. Colovai, Foxwell N. Emmons, Govind Bhagat & Bachir Alobeid. (2010) Hematogones: a review and update. Leukemia & Lymphoma 51:1, pages 10-19.
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Ol'ga Babušíková, Tatiana Železníková, Gabriela Kirschnerová & Esko Kankuri. (2008) Hematogones in acute leukemia during and after therapy. Leukemia & Lymphoma 49:10, pages 1935-1944.
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Sevgi Yetgin, Barış Kuşkonmaz, Selin Aytaç & Betül Tavil. (2007) AN UNUSUAL CASE OF REACTIVE LYMPHOCYTOSIS MIMICKING ACUTE LEUKEMIA. Pediatric Hematology and Oncology 24:2, pages 129-135.
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Anil Handoo & Tina Dadu. (2018) Flow Cytometry in Pediatric Malignancies. Indian Pediatrics 55:1, pages 55-62.
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Nicola A. Hanania & Paula Busse. 2016. Advances in Geroscience. Advances in Geroscience 397 428 .
David Wu, Brent L. Wood & Jonathan R. Fromm. 2016. Molecular Pathology in Cancer Research. Molecular Pathology in Cancer Research 279 316 .
Denise Lee, George Grigoriadis & David Westerman. (2015) The role of multiparametric flow cytometry in the detection of minimal residual disease in acute leukaemia. Pathology 47:7, pages 609-621.
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Maura Rosane Valério Ikoma, Miriam Perlingeiro Beltrame, Silvia Inês Alejandra Cordoba Pires Ferreira, Elizabeth Xisto Souto, Mariester Malvezzi & Mihoko Yamamoto. (2015) Proposal for the standardization of flow cytometry protocols to detect minimal residual disease in acute lymphoblastic leukemia. Revista Brasileira de Hematologia e Hemoterapia 37:6, pages 406-413.
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Michele Paessler. (2015) Case study interpretation-Fort Lauderdale: Case 2. Cytometry Part B: Clinical Cytometry 88:6, pages 400-403.
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Sanam Loghavi, Jeffery L. KutokJeffrey L. Jorgensen. (2015) B-Acute Lymphoblastic Leukemia/Lymphoblastic Lymphoma. American Journal of Clinical Pathology 144:3, pages 393-410.
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Aaron C. Shaver, Bruce W. Greig, Claudio A. MosseAdam C. Seegmiller. (2015) B-ALL Minimal Residual Disease Flow Cytometry. American Journal of Clinical Pathology 143:5, pages 716-724.
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Nora C. J. Sun, Jun Wang, Xiaohui Sheila Zhao & Eric F. Glassy. 2015. Silverberg's Principles and Practice of Surgical Pathology and Cytopathology. Silverberg's Principles and Practice of Surgical Pathology and Cytopathology 813 942 .
. 2014. Morphology of Blood Disorders. Morphology of Blood Disorders 553 586 .
Tajana Juranovic, Chibuzo C. O'Suoji, Theru A. Sivakumaran, Kejian Zhang, Oscar C. Estallila & Tomislav M. Jelic. (2016) Hematogones in the Peripheral Blood of a 5½-Month-Old Boy with Cyclic Neutropenia Due to Heterozygous, Novel ELANE Gene Mutation p.Q97P, c.290 A>C . Pediatric and Developmental Pathology 17:5, pages 393-399.
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Ł. Sędek, J. Bulsa, A. Sonsala, M. Twardoch, M. Wieczorek, I. Malinowska, K. Derwich, M. Niedźwiecki, G. Sobol-Milejska, J. R. Kowalczyk, B. Mazur & T. Szczepański. (2014) The immunophenotypes of blast cells in B-cell precursor acute lymphoblastic leukemia: How different are they from their normal counterparts?. Cytometry Part B: Clinical Cytometry 86:5, pages 329-339.
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Aharon G. Freud, Jianhua Yu & Michael A. Caligiuri. (2014) Human natural killer cell development in secondary lymphoid tissues. Seminars in Immunology 26:2, pages 132-137.
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S.P. Chantepie, E. Cornet, V. Salaün & O. Reman. (2013) Hematogones: An overview. Leukemia Research 37:11, pages 1404-1411.
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S Agrawal, S A B C Smith, S G Tangye & W A Sewell. (2013) Transitional B cell subsets in human bone marrow. Clinical and Experimental Immunology 174:1, pages 53-59.
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Jine Zheng, Wen Du, Junxia Yao, Yong You, Weiming Li, Yanli He, Xiaoqing Li, Wei Liu, Yaohui Wu, Yanjie Hu, Runming Jin, Ping Zou, Shiang Huang, Yu Hu & Min Zhang. (2013) Analysis of hematogones in bone marrow from acute myeloid leukaemia cases posttherapy. European Journal of Clinical Investigation, pages n/a-n/a.
Crossref
Mike Leach, Mark Drummond & Allyson Doig. 2013. Practical Flow Cytometry in Haematology Diagnosis. Practical Flow Cytometry in Haematology Diagnosis 31 42 .
Marieta Anton-Harisi, Varvara Douna, Margarita Baka, Marina Servitzoglou, Helen V. Kosmidis, Helen Georgouli & Theodora Anastasiou. (2012) Acute Megakaryoblastic Leukemia With Increased Hematogones in Children. Journal of Pediatric Hematology/Oncology 34:8, pages e337-e340.
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Frederick G. Behm. 2013. Childhood Leukemias. Childhood Leukemias 72 112 .
Theodore Honebrink, Vanessa Dayton, Michael J. Burke, Karen Larsen, Qing Cao, Claudio Brunstein, Daniel Weisdorf, Jeffery S. Miller, John E. Wagner & Michael R. Verneris. (2012) Impact of Bone Marrow Hematogones on Umbilical Cord Blood Transplantation Outcomes in Patients with Acute Myeloid Leukemia. Biology of Blood and Marrow Transplantation 18:6, pages 930-936.
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Daisy Alapat, Jean Coviello-Malle, Rebecca Owens, Pingping Qu, Bart Barlogie, John D. Shaughnessy & Robert B. Lorsbach. (2012) Diagnostic Usefulness and Prognostic Impact of CD200 Expression in Lymphoid Malignancies and Plasma Cell Myeloma. American Journal of Clinical Pathology 137:1, pages 93-100.
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Sophia L. Yohe & Robert W. McKenna. 2012. Bone Marrow Lymphoid Infiltrates. Bone Marrow Lymphoid Infiltrates 145 169 .
Dimitra Anagnostou. 2012. Bone Marrow Lymphoid Infiltrates. Bone Marrow Lymphoid Infiltrates 113 144 .
Claudio Ortolani. 2011. Flow Cytometry of Hematological Malignancies. Flow Cytometry of Hematological Malignancies 1 157 .
M C Béné, T Nebe, P Bettelheim, B Buldini, H Bumbea, W Kern, F Lacombe, P Lemez, I Marinov, E Matutes, M Maynadié, U Oelschlagel, A Orfao, R Schabath, M Solenthaler, G Tschurtschenthaler, A M Vladareanu, G Zini, G C Faure & A Porwit. (2011) Immunophenotyping of acute leukemia and lymphoproliferative disorders: a consensus proposal of the European LeukemiaNet Work Package 10. Leukemia 25:4, pages 567-574.
Crossref
Deborah W. Sevilla, Nader Kim El-Mallawany, Foxwell N. Emmons, Suzy Alexander, Govind Bhagat & Bachir Alobeid. (2012) Spectrum of Childhood Epstein-Barr Virus–Associated T-Cell Proliferations and Bone Marrow Findings. Pediatric and Developmental Pathology 14:1, pages 28-37.
Crossref
Kyung Nam KohMeerim ParkBo Eun KimHo Joon ImChan-Jeoung ParkSeongsoo JangHyun Sook ChiJong Jin Seo. (2010) Prognostic significance of minimal residual disease detected by a simplified flow cytometric assay during remission induction chemotherapy in children with acute lymphoblastic leukemia. Korean Journal of Pediatrics 53:11, pages 957.
Crossref
Adam C. Seegmiller, Steven H. Kroft, Nitin J. Karandikar & Robert W. McKenna. (2009) Characterization of Immunophenotypic Aberrancies in 200 Cases of B Acute Lymphoblastic Leukemia. American Journal of Clinical Pathology 132:6, pages 940-949.
Crossref
Franklin S. Fuda, Nitin J. Karandikar & Weina Chen. (2009) Significant CD5 Expression on Normal Stage 3 Hematogones and Mature B Lymphocytes in Bone Marrow. American Journal of Clinical Pathology 132:5, pages 733-737.
Crossref
Nagwa M. Hassanein, Felisa Alcancia, Kathryn R. Perkinson, Patrick J. Buckley & Anand S. Lagoo. (2009) Distinct Expression Patterns of CD123 and CD34 on Normal Bone Marrow B-Cell Precursors (“Hematogones”) and B Lymphoblastic Leukemia Blasts. American Journal of Clinical Pathology 132:4, pages 573-580.
Crossref
Maria Proytcheva. 2008. Handbook of Human Immunology, Second Edition. Handbook of Human Immunology, Second Edition 221 256 .
Bienvenu Massamba Lebwaze, Agnès Le Tourneau, Bernard Rio, Jean-Yves Perrot, Laurence Heuberger, Jean-Marie Mpolesha Kabongo, Raphaël Mbowa Kalengayi, Thierry Molina, Jacques Diebold & Josée Audouin. (2008) Aspect histopathologique d’une hyperplasie médullaire osseuse des hématogones (précurseurs lymphoïdes B médullaires) au cours d’une myélofibrose idiopathique traitée. Annales de Pathologie 28:1, pages 27-31.
Crossref
Soyoung ShinJimin KahngMyungshin KimJihyang LimYounggoo KimKyungja Han. (2008) Distribution of Antigenic Aberration in the Bone Marrow of Acute Leukemia in Complete Remission. The Korean Journal of Laboratory Medicine 28:1, pages 1.
Crossref
Faramarz Naeim. 2008. Hematopathology. Hematopathology 1 26 .
Fiona E. Craig. (2007) Flow Cytometric Evaluation of B-cell Lymphoid Neoplasms. Clinics in Laboratory Medicine 27:3, pages 487-512.
Crossref
Cherie H. Dunphy, Dennis P. O'Malley, Sherrie L. Perkins & Chung-Che Chang. (2007) Analysis of Immunohistochemical Markers in Bone Marrow Sections to Evaluate for Myelodysplastic Syndromes and Acute Myeloid Leukemias. Applied Immunohistochemistry & Molecular Morphology 15:2, pages 154-159.
Crossref
Jimin Kahng, So-Young Shin & Kyungja Han. (2007) Proportions of Cells Expressing CD38-/CD34+, CD38+/CD34+, CD19+/CD34+, or CD13,33+/CD34+ in the Regenerating Bone Marrows During Complete Remission of Acute Leukemia or After Bone Marrow Transplantation. The Korean Journal of Laboratory Medicine 27:6, pages 406.
Crossref
Fred G. Behm. 2010. Childhood Leukemias. Childhood Leukemias 150 209 .
Jeong-Hun KimWoo-In LeeSu-Yon ParkHwi-Joong YoonSo-Young KangJin-Tae Suh. (2006) A Case of Acute Lymphoblastic Leukemia with Maturation. The Korean Journal of Hematology 41:1, pages 46.
Crossref
Abigail S. Kelliher, David W. Parent, David C. Anderson, Michelle E. Dorn, Jessica L. Hahn, Sara Eapen & Frederic I. Preffer. (2005) Novel use of the BD FACS™ SPA to automate custom monoclonal antibody panel preparations for immunophenotyping. Cytometry Part B: Clinical Cytometry 66B:1, pages 40-45.
Crossref
Bo-Yee Ngan, Jo-Anne Vergilio & Megan S. Lim. 2016. Essentials of Surgical Pediatric Pathology. Essentials of Surgical Pediatric Pathology 228 274 .

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