279
Views
69
CrossRef citations to date
0
Altmetric
Original

The potential pitfalls of using 1,1-diphenyl-2-picrylhydrazyl to characterize antioxidants in mixed water solvents

, , &
Pages 379-390 | Received 27 Mar 2006, Published online: 07 Jul 2009

Keep up to date with the latest research on this topic with citation updates for this article.

Read on this site (3)

Andrej Staško, Vlasta Brezová, Michal Zalibera, Stanislav Biskupič & Karol Ondriaš. (2009) Electron transfer: A primary step in the reactions of sodium hydrosulphide, an H2S/HS− donor. Free Radical Research 43:6, pages 581-593.
Read now

Articles from other publishers (66)

Yiyun Zhu, Kang Wang, Xinyi Jia, Caili Fu, Haining Yu & Yipeng Wang. (2023) Antioxidant peptides, the guardian of life from oxidative stress. Medicinal Research Reviews 44:1, pages 275-364.
Crossref
Song Cheng, Yutao Liu, Xinyi Liu, Changqing Zhu, Jingyang Luo, Jiashun Cao & Fuqiang Liu. (2023) Co3O4 nanosheets modified by Ni@C nanoparticles as effective flow-through electrode for electro-activation of PMS: Ni@C as electron shuttle for accelerated Co3+/Co2+ cycle in bulk Co3O4. Chemical Engineering Journal 472, pages 145084.
Crossref
Mauro Gisbert, Daniel Franco, Jorge Sineiro & Ramón Moreira. (2023) Antioxidant and Antidiabetic Properties of Phlorotannins from Ascophyllum nodosum Seaweed Extracts. Molecules 28:13, pages 4937.
Crossref
YOGESH CHANDRA NAINWAL. (2023) PHYTOCHEMICAL SCREENING AND EVALUATION OF FREE RADICAL SCAVENGING ACTIVITY OF EXTRACT PREPARED FROM LEAVES OF DIGITALIS PURPUREA. Asian Journal of Pharmaceutical and Clinical Research, pages 20-23.
Crossref
Rabinder Singh, Mohammed Rehmanji, Asha A. Nesamma & Pannaga P. Jutur. 2023. Marine Antioxidants. Marine Antioxidants 161 184 .
Marios C. Christodoulou, Jose C. Orellana Palacios, Golnaz Hesami, Shima Jafarzadeh, José M. Lorenzo, Rubén Domínguez, Andres Moreno & Milad Hadidi. (2022) Spectrophotometric Methods for Measurement of Antioxidant Activity in Food and Pharmaceuticals. Antioxidants 11:11, pages 2213.
Crossref
Linghong Shi, Wanrong Zhao, Zihong Yang, Vigasini Subbiah & Hafiz Ansar Rasul Suleria. (2022) Extraction and characterization of phenolic compounds and their potential antioxidant activities. Environmental Science and Pollution Research 29:54, pages 81112-81129.
Crossref
Izabela Sadowska-Bartosz & Grzegorz Bartosz. (2022) Evaluation of The Antioxidant Capacity of Food Products: Methods, Applications and Limitations. Processes 10:10, pages 2031.
Crossref
Elena Gerasimova, Elena Gazizullina, Sofya Kolbaczkaya & Alla Ivanova. (2022) The Novel Potentiometric Approach to Antioxidant Capacity Assay Based on the Reaction with Stable Radical 2,2′-diphenyl-1-picrylhydrazyl. Antioxidants 11:10, pages 1974.
Crossref
Renan Danielski, Simone Mazzutti, Sandra Regina Salvador Ferreira, Luciano Vitali & Jane Mara Block. (2022) A non‐conventional approach for obtaining phenolic antioxidants from red guava ( Psidium guajava L.) by‐products . Journal of Food Processing and Preservation 46:6.
Crossref
Lara Campos, Luana Seixas, Susana Dias, António M. Peres, Ana C. A. Veloso & Marta Henriques. (2022) Effect of Extraction Method on the Bioactive Composition, Antimicrobial Activity and Phytotoxicity of Pomegranate By-Products. Foods 11:7, pages 992.
Crossref
Paweł Przybylski, Adrian Konopko, Piotr Łętowski, Katarzyna Jodko-Piórecka & Grzegorz Litwinienko. (2022) Concentration-dependent HAT/ET mechanism of the reaction of phenols with 2,2-diphenyl-1-picrylhydrazyl (dpph˙) in methanol. RSC Advances 12:13, pages 8131-8136.
Crossref
Talyta Mayara Silva Torres, Jhonyson Arruda Carvalho Guedes, Edy Sousa de Brito, Simone Mazzutti & Sandra Regina Salvador Ferreira. (2022) High-pressure biorefining of ora-pro-nobis (Pereskia aculeata). The Journal of Supercritical Fluids 181, pages 105514.
Crossref
Talyta Mayara Silva Torres, Gerardo Álvarez-Rivera, Simone Mazzutti, Jose David Sánchez-Martínez, Alejandro Cifuentes, Elena Ibáñez & Sandra Regina Salvador Ferreira. (2022) Neuroprotective potential of extracts from leaves of ora-pro-nobis (Pereskia aculeata) recovered by clean compressed fluids. The Journal of Supercritical Fluids 179, pages 105390.
Crossref
Mindaugas Liaudanskas, Vaidotas Žvikas & Vilma Petrikaitė. (2021) The Potential of Dietary Antioxidants from a Series of Plant Extracts as Anticancer Agents against Melanoma, Glioblastoma, and Breast Cancer. Antioxidants 10:7, pages 1115.
Crossref
Kristina Perminaite, Mindaugas Marksa, Monika Stančiauskaitė, Tadas Juknius, Aidas Grigonis & Kristina Ramanauskiene. (2021) Formulation of Ocular In Situ Gels with Lithuanian Royal Jelly and Their Biopharmaceutical Evaluation In Vitro. Molecules 26:12, pages 3552.
Crossref
Irina Georgiana Munteanu & Constantin Apetrei. (2021) Analytical Methods Used in Determining Antioxidant Activity: A Review. International Journal of Molecular Sciences 22:7, pages 3380.
Crossref
Renan da Silva Lima, Itaciara Larroza Nunes & Jane Mara Block. (2019) Ultrasound-Assisted Extraction for the Recovery of Carotenoids from Guava’s Pulp and Waste Powders. Plant Foods for Human Nutrition 75:1, pages 63-69.
Crossref
İlhami Gulcin. (2020) Antioxidants and antioxidant methods: an updated overview. Archives of Toxicology 94:3, pages 651-715.
Crossref
Daniel Goujot, Marie-Elisabeth Cuvelier, Paola Soto & Francis Courtois. (2019) A stoichio-kinetic model for a -ferulic acid reaction . Talanta 196, pages 284-292.
Crossref
E. Halevas, C.M. Nday, D. Eleftheriadou, G. Jackson, V. Psycharis, C.P. Raptopoulou, D.G. Reid, K. Ypsilantis, G. Litsardakis & A. Salifoglou. (2019) Synthesis and encapsulation of V(IV,V) compounds in silica nanoparticles targeting development of antioxidant and antiradical nanomaterials. Journal of Inorganic Biochemistry 194, pages 180-199.
Crossref
Martin MichalíkJán RimarčíkVladimír LukešErik Klein. (2019) Thermodynamics of primary antioxidant action of flavonols in polar solvents. Acta Chimica Slovaca 12:1, pages 108-118.
Crossref
Renan da Silva Lima, Sandra Regina Salvador Ferreira, Luciano Vitali & Jane Mara Block. (2019) May the superfruit red guava and its processing waste be a potential ingredient in functional foods?. Food Research International 115, pages 451-459.
Crossref
B. M. Gnanamangai, S. Saranya, P. Ponmurugan, S. Kavitha, Sudhagar Pitchaimuthu & P. Divya. 2019. Sustainable Agriculture Reviews 34. Sustainable Agriculture Reviews 34 19 40 .
Elena González-Burgos, Mindaugas Liaudanskas, Jonas Viškelis, Vaidotas Žvikas, Valdimaras Janulis & M. Pilar Gómez-Serranillos. (2018) Antioxidant activity, neuroprotective properties and bioactive constituents analysis of varying polarity extracts from Eucalyptus globulus leaves. Journal of Food and Drug Analysis 26:4, pages 1293-1302.
Crossref
Nikolaos Nenadis & Maria Z. Tsimidou. 2018. Measurement of Antioxidant Activity & Capacity. Measurement of Antioxidant Activity & Capacity 141 164 .
Katarzyna Jodko-Piórecka, Jakub Cedrowski & Grzegorz Litwinienko. 2018. Measurement of Antioxidant Activity & Capacity. Measurement of Antioxidant Activity & Capacity 225 272 .
Ahmad Bayat & Alireza Fattahi. (2017) A quantum chemical study on the OH radical quenching by natural antioxidant fisetin. Journal of Physical Organic Chemistry 30:11, pages e3692.
Crossref
Hong Yang, Xuejia Xue, Huan Li, Su Chin Tay-Chan, Seng Poon Ong & Edmund Feng Tian. (2017) A new parameter to simultaneously assess antioxidant activity for multiple phenolic compounds present in food products. Food Chemistry 229, pages 215-222.
Crossref
Chia Miang Khor, Wai Kiong Ng, Kok Ping Chan & Yuancai Dong. (2017) Preparation and characterization of quercetin/dietary fiber nanoformulations. Carbohydrate Polymers 161, pages 109-117.
Crossref
Zuzana Machatová, Zuzana Barbieriková, Peter Poliak, Viera Jančovičová, Vladimír Lukeš & Vlasta Brezová. (2016) Study of natural anthraquinone colorants by EPR and UV/vis spectroscopy. Dyes and Pigments 132, pages 79-93.
Crossref
Erik Klein, Ján Rimarčík, Erika Senajová, Adam Vagánek & Jozef Lengyel. (2016) Deprotonation of flavonoids severely alters the thermodynamics of the hydrogen atom transfer. Computational and Theoretical Chemistry 1085, pages 7-17.
Crossref
Jiyuan Liu, Jing Gan, Yongjian Yu, Shenghu Zhu, Lijun Yin & Yongqiang Cheng. (2016) Effect of laboratory-scale decoction on the antioxidative activity of Zhenjiang Aromatic Vinegar: The contribution of melanoidins. Journal of Functional Foods 21, pages 75-86.
Crossref
Ryoko YAMAUCHI, Saya ISHII, Yuri KUSABA, Hiroshi KOBAYASHI, Tomoko SHIMAMURA, Hiroyuki UKEDA, Hiroshi AKIYAMA & Hiroya ISHIKAWA. (2016) Solvent Effect on the DPPH Radical Scavenging Assay for Evaluating Natural Antioxidants as Food Additives酸化防止剤力価評価を目的としたDPPHラジカル消去能測定におよぼす反応溶媒の影響. Food Preservation Science 42:5, pages 189-196.
Crossref
Zoran MARKOVIC, Jelena DOROVIC, Jasmina M. DIMITRIC MARKOVIC, Radomir BIOCANIN & Dragan AMIC. (2016) Comparative density functional study of antioxidative activity of the hydroxybenzoic acids and their anions. TURKISH JOURNAL OF CHEMISTRY 40, pages 499-509.
Crossref
Sergey K. Filippov, Olga Yu. Sergeeva, Petr S. Vlasov, Margarita S. Zavyalova, Galina B. Belostotskaya, Vasil M. Garamus, Raisa S. Khrustaleva, Petr Stepanek & Nina S. Domnina. (2015) Modified hydroxyethyl starch protects cells from oxidative damage. Carbohydrate Polymers 134, pages 314-323.
Crossref
Lin Zheng, Lianzhu Lin, Guowan Su, Qiangzhong Zhao & Mouming Zhao. (2015) Pitfalls of using 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay to assess the radical scavenging activity of peptides: Its susceptibility to interference and low reactivity towards peptides. Food Research International 76, pages 359-365.
Crossref
Katarzyna Jodko-Piórecka & Grzegorz Litwinienko. (2015) Antioxidant activity of dopamine and L-DOPA in lipid micelles and their cooperation with an analogue of α-tocopherol. Free Radical Biology and Medicine 83, pages 1-11.
Crossref
Ali Benayahoum, Habiba Amira-Guebailia & Omar Houache. (2015) On the role of ethylene bridge elongation in the antioxidant activity of polyhydroxylated stilbenes: A theoretical approach. Comptes Rendus Chimie 18:2, pages 149-159.
Crossref
Kuheli Das, Uttam Panda, Amitabha Datta, Suman Roy, Sudipa Mondal, Chiara Massera, Tulin Askun, Pinar Celikboyun, Eugenio Garribba, Chittaranjan Sinha, Kushi Anand, Takashiro Akitsu & Kana Kobayashi. (2015) An enolato-bridged dinuclear Cu( ii ) complex with a coumarin-assisted precursor: a spectral, magnetic and biological study . New Journal of Chemistry 39:9, pages 7309-7321.
Crossref
Owen Kenny, Nigel P. Brunton & Thomas J. Smyth. 2015. Natural Products From Marine Algae. Natural Products From Marine Algae 375 402 .
Adam Vagánek, Ján Rimarčík, Kamila Dropková, Jozef Lengyel & Erik Klein. (2014) Reaction enthalpies of OH bonds splitting-off in flavonoids: The role of non-polar and polar solvent. Computational and Theoretical Chemistry 1050, pages 31-38.
Crossref
Ali Benayahoum, Habiba Amira-Guebailia & Omar Houache. (2014) Homolytic and heterolytic O–H bond cleavage in trans-resveratrol and some phenantrene analogs: A theoretical study. Computational and Theoretical Chemistry 1037, pages 1-9.
Crossref
L. M. Magalhães, I. I. Ramos, S. Reis & M. A. Segundo. (2014) Antioxidant profile of commercial oenological tannins determined by multiple chemical assays. Australian Journal of Grape and Wine Research 20:1, pages 72-79.
Crossref
Fatima Abderrahim, Silvia M. Arribas, M. Carmen Gonzalez & Luis Condezo-Hoyos. (2013) Rapid high-throughput assay to assess scavenging capacity index using DPPH. Food Chemistry 141:2, pages 788-794.
Crossref
Jozef Lengyel, Ján Rimarčík, Adam Vagánek & Erik Klein. (2013) On the radical scavenging activity of isoflavones: thermodynamics of O–H bond cleavage. Physical Chemistry Chemical Physics 15:26, pages 10895.
Crossref
Krystyna Pyrzynska & Anna Pękal. (2013) Application of free radical diphenylpicrylhydrazyl (DPPH) to estimate the antioxidant capacity of food samples. Analytical Methods 5:17, pages 4288.
Crossref
Laura Bertalanič, Tatjana Košmerl, Nataša Poklar Ulrih & Blaž Cigić. (2012) Influence of Solvent Composition on Antioxidant Potential of Model Polyphenols and Red Wines Determined with 2,2-Diphenyl-1-picrylhydrazyl. Journal of Agricultural and Food Chemistry 60:50, pages 12282-12288.
Crossref
Zuzana RÉBLOVÁ, Jakub FIŠNAR, Dana TICHOVSKÁ, Marek DOLEŽAL & Kristýna JOUDALOVÁ. (2012) Ability of phenolic acids to protect naturally present alfa-tocopherol during the heating of plant oils. Czech Journal of Food Sciences 30:4, pages 351-357.
Crossref
Daniele Sanna, Giovanna Delogu, Maurizio Mulas, Mario Schirra & Angela Fadda. (2011) Determination of Free Radical Scavenging Activity of Plant Extracts Through DPPH Assay: An EPR and UV–Vis Study. Food Analytical Methods 5:4, pages 759-766.
Crossref
Adam Vagánek, Ján Rimarčík, Vladimír Lukeš & Erik Klein. (2012) On the energetics of homolytic and heterolytic OH bond cleavage in flavonoids. Computational and Theoretical Chemistry 991, pages 192-200.
Crossref
M. Čaplovičová, P. Billik, Ľ. Čaplovič, V. Brezová, T. Turáni, G. Plesch & P. Fejdi. (2012) On the true morphology of highly photoactive anatase TiO2 nanocrystals. Applied Catalysis B: Environmental 117-118, pages 224-235.
Crossref
S. Rodríguez-Rojo, A. Visentin, D. Maestri & M.J. Cocero. (2012) Assisted extraction of rosemary antioxidants with green solvents. Journal of Food Engineering 109:1, pages 98-103.
Crossref
İlhami Gülçin. (2011) Antioxidant activity of food constituents: an overview. Archives of Toxicology 86:3, pages 345-391.
Crossref
Denys J. CharlesDenys J. Charles. 2013. Antioxidant Properties of Spices, Herbs and Other Sources. Antioxidant Properties of Spices, Herbs and Other Sources 9 38 .
Cristina Sgherri, Calogero Pinzino, Flavia Navari-Izzo & Riccardo Izzo. (2011) Contribution of major lipophilic antioxidants to the antioxidant activity of basil extracts: an EPR study. Journal of the Science of Food and Agriculture 91:6, pages 1128-1134.
Crossref
Raquel BridiCarolina AliagaAlexis AspéeElsa AbuinEduardo Lissi. (2011) Distribution and reactivity of gallates toward galvinoxyl radicals in SDS micellar solutions — Effect of the alkyl chain length. Canadian Journal of Chemistry 89:2, pages 181-185.
Crossref
Zuzana Hricovíniová, Michal Zalibera & Miloš Hricovíni. (2010) Microwave-assisted regioselective synthesis and isomerization of 6-O-vanillyl- and 6-O-iso-vanillyl hexoses and studies of their activities as radical scavengers by EPR spectroscopy. Tetrahedron: Asymmetry 21:6, pages 756-761.
Crossref
N. Nenadis & M.Z. Tsimidou. 2010. Oxidation in Foods and Beverages and Antioxidant Applications. Oxidation in Foods and Beverages and Antioxidant Applications 332 367 .
A. M. Campos, E. Ponce & E. A. Lissi. (2009) Effects of alkyl chain lengths of gallates upon their distribution and reactivity towards diphenylpicryl hydracil radicals in Triton X-100 micellar solutions. Journal of Physical Organic Chemistry 22:12, pages 1208-1211.
Crossref
Vlasta Brezová, Anna Šlebodová & Andrej Staško. (2009) Coffee as a source of antioxidants: An EPR study. Food Chemistry 114:3, pages 859-868.
Crossref
Malgorzata Musialik, Rafal Kuzmicz, Tomasz S. Pawłowski & Grzegorz Litwinienko. (2009) Acidity of Hydroxyl Groups: An Overlooked Influence on Antiradical Properties of Flavonoids. The Journal of Organic Chemistry 74:7, pages 2699-2709.
Crossref
Michal Zalibera, Andrej Staško, Anna Šlebodová, Viera Jančovičová, Tatiana Čermáková & Vlasta Brezová. (2008) Antioxidant and radical-scavenging activities of Slovak honeys – An electron paramagnetic resonance study. Food Chemistry 110:2, pages 512-521.
Crossref
Naseem Saud Ahmad, Muhammad Farman, Muzammil Hasan Najmi, Kouser Bashir Mian & Aurangzeb Hasan. (2008) Pharmacological basis for use of Pistacia integerrima leaves in hyperuricemia and gout. Journal of Ethnopharmacology 117:3, pages 478-482.
Crossref
Luís M. Magalhães, Marcela A. Segundo, Salette Reis & José L.F.C. Lima. (2008) Methodological aspects about in vitro evaluation of antioxidant properties. Analytica Chimica Acta 613:1, pages 1-19.
Crossref
Erik Klein, Vladimír Lukeš & Michal Ilčin. (2007) DFT/B3LYP study of tocopherols and chromans antioxidant action energetics. Chemical Physics 336:1, pages 51-57.
Crossref

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.