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Cytotherapy Volume 11, 2009 - Issue 5
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CD34 Enumeration

Comparison of two single-platform ISHAGE-based CD34 enumeration protocols on BD FACSCalibur and FACSCanto flow cytometers

D. Robert Sutherland Clinical Flow Cytometry Laboratory, University Health Network, Toronto, CanadaCorrespondence[email protected]
,
Rakesh Nayyar Clinical Flow Cytometry Laboratory, University Health Network, Toronto, Canada
,
Erica Acton Clinical Flow Cytometry Laboratory, University Health Network, Toronto, Canada
,
Angeline Giftakis Cellular Therapy Laboratory, CancerCare Manitoba, Canada
,
Sue Dean BD Biosciences, San Jose, California, USA
&
Victoria L. Mosiman BD Biosciences, San Jose, California, USA
Pages 595-605 | Published online: 16 Sep 2009

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Tomislav M Jelic, Oscar C Estalilla, Jeffrey A Vos, Gary Harvey, Caitlin J Stricker, Ayodele O Adelanwa, Ahmed A Khalid & Milton J Plata. (2023) Flow Cytometric Enumeration of Peripheral Blood CD34+ Cells Predicts Bone Marrow Pathology in Patients with Less Than 1% Blasts by Manual Count. Journal of Blood Medicine 14, pages 519-535.
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Gerhard Fritsch, Volker Witt, Hans Peter Spengler, Josefa Pichler, Daniela Scharner, Elke Zipperer, Dijana Trbojevic, Julia Stemberger, René Geyeregger, Muy-Kheng Maria Tea & Dieter Printz. (2012) Robust Multi-Parameter Single-Platform Quantification of Myeloid and B-Lymphoid CD34 Progenitor Cells in All Clinical CD34 Cell Sources and in Thawed PBSC. Pediatric Hematology and Oncology 29:7, pages 595-610.
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Samira Mortazavi, Farid Azmoudeh Ardalan, Seyyed Reza Safaee Nodehi, Fahimeh Firouzjaie Karder & Nafisehsadat Miraliakbari. (2012) True volumetric method for flow cytometric enumeration of CD34 + stem cells and its agreement with a standard bead-based single-platform protocol. Cytotherapy 14:5, pages 621-629.
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Ruud Hulspas. (2012) New ways to count CD34. Cytotherapy 14:5, pages 639-640.
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Richard Cuthbert, Sally A Boxall, Hiang Boon Tan, Peter V. Giannoudis, Dennis McGonagle & Elena Jones. (2012) Single-platform quality control assay to quantify multipotential stromal cells in bone marrow aspirates prior to bulk manufacture or direct therapeutic use. Cytotherapy 14:4, pages 431-440.
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Giovanna Cameron, Adisak Tantiworawit, Mike Halpenny, Brenda Letcher, Sue Berrigan, Karen Hindmarsh, Angeline Giftakis, Johanne Fortier, Pamela O'Hoski & Donna Hogge. (2011) Cryopreserved mobilized autologous blood progenitors stored for more than 2 years successfully support blood count recovery after high-dose chemotherapy. Cytotherapy 13:7, pages 856-863.
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Katrin Dauber, Daniela Becker, Marcus Odendahl, Erhard Seifried, Halvard Bonig & Torsten Tonn. (2011) Enumeration of viable CD34+ cells by flow cytometry in blood, bone marrow and cord blood: results of a study of the novel BD™ stem cell enumeration kit. Cytotherapy 13:4, pages 449-458.
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Adrian P. Gee, Sara Richman, April Durett, David McKenna, Jay Traverse, Timothy Henry, Diann Fisk, Carl Pepine, Jeannette Bloom, James Willerson, Karen Prater, David Zhao, Jane Reese Koç, Steven Ellis, Doris Taylor, Christopher Cogle, Lemuel Moyé, Robert Simari & Sonia Skarlatos. (2010) Multicenter cell processing for cardiovascular regenerative medicine applications: the Cardiovascular Cell Therapy Research Network (CCTRN) experience. Cytotherapy 12:5, pages 684-691.
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