Advanced search
Publication Cover
Cell Communication & Adhesion Volume 15, 2008 - Issue 1-2
Journal homepage
1,532
Views
64
CrossRef citations to date
0
Altmetric
Original

Currently Used Methods for Identification and Characterization of Hemichannels

Kurt A. Schalper Departamento de Ciencias Fisiológicas, Núcleo de Immunología e inmuroterapia, Pontificia Universidad Católica de Chile, Santiago, Chile
,
Nicolás Palacios-Prado Departamento de Ciencias Fisiológicas, Núcleo de Immunología e inmuroterapia, Pontificia Universidad Católica de Chile, Santiago, Chile
,
Juan A. Orellana Departamento de Ciencias Fisiológicas, Núcleo de Immunología e inmuroterapia, Pontificia Universidad Católica de Chile, Santiago, Chile
&
Juan C. Sáez Departamento de Ciencias Fisiológicas, Núcleo de Immunología e inmuroterapia, Pontificia Universidad Católica de Chile, Santiago, Chile
Pages 207-218 | Received 07 Jan 2008, Accepted 25 Jan 2008, Published online: 11 Jul 2009

Citations (64)

Keep up to date with the latest research on this topic with citation updates for this article.

Subscribe to citation updates

Read on this site (1)

Kenji F Shoji, Pablo J Sáez, Paloma A Harcha, Hector L Aguila & Juan C Sáez. (2014) Pannexin1 channels act downstream of P2X7 receptors in ATP-induced murine T-cell death. Channels 8:2, pages 142-156.
Read now

Articles from other publishers (63)

Juan Prieto-Villalobos, Claudia M. Lucero, Maximiliano Rovegno, Gonzalo I. Gómez, Mauricio A. Retamal & Juan A. Orellana. (2023) SARS-CoV-2 spike protein S1 activates Cx43 hemichannels and disturbs intracellular Ca2+ dynamics. Biological Research 56:1.
Crossref
Luis A. Cea, Walter Vásquez, Romina Hernández-Salinas, Alejandra Z. Vielma, Mario Castillo-Ruiz, Victoria Velarde, Magdiel Salgado & Juan C. Sáez. (2023) Skeletal Muscle Atrophy Induced by Diabetes Is Mediated by Non-Selective Channels and Prevented by Boldine. Biomolecules 13:4, pages 708.
Crossref
Ivanka Jiménez-Dinamarca, Rachel Reyes-Lizana, Yordan Lemunao-Inostroza, Kevin Cárdenas, Raimundo Castro-Lazo, Francisca Peña, Claudia M. Lucero, Juan Prieto-Villalobos, Mauricio Antonio Retamal, Juan Andrés Orellana & Jimmy Stehberg. (2022) GABAergic Regulation of Astroglial Gliotransmission through Cx43 Hemichannels. International Journal of Molecular Sciences 23:21, pages 13625.
Crossref
Julia Fadjukov, Sophia Wienbar, Satu Hakanen, Vesa Aho, Maija Vihinen-Ranta, Teemu O. Ihalainen, Gregory W. Schwartz & Soile Nymark. (2022) Gap junctions and connexin hemichannels both contribute to the electrical properties of retinal pigment epithelium. Journal of General Physiology 154:4.
Crossref
Katarina Andelova, Tamara Egan Benova, Barbara Szeiffova Bacova, Matus Sykora, Natalia Jorgelina Prado, Emiliano Raul Diez, Peter Hlivak & Narcis Tribulova. (2020) Cardiac Connexin-43 Hemichannels and Pannexin1 Channels: Provocative Antiarrhythmic Targets. International Journal of Molecular Sciences 22:1, pages 260.
Crossref
Juan C. Sáez, Aníbal A. Vargas, Diego E. Hernández, Fernando C. Ortiz, Christian Giaume & Juan A. Orellana. (2020) Permeation of Molecules through Astroglial Connexin 43 Hemichannels Is Modulated by Cytokines with Parameters Depending on the Permeant Species. International Journal of Molecular Sciences 21:11, pages 3970.
Crossref
Aneta Dydowiczová, Ondřej Brózman, Pavel Babica & Iva Sovadinová. (2020) Improved multiparametric scrape loading-dye transfer assay for a simultaneous high-throughput analysis of gap junctional intercellular communication, cell density and viability. Scientific Reports 10:1.
Crossref
Jianfeng Zhao, Fan Zhao, Miaoyong Ye, Ke Ma, Wenjie Huang, Le Qian, Xiaojun Huang, Huiyin Fu & Bodong Lv. (2020) Salidroside Attenuates Hypoxia-Induced Expression of Connexin 43 in Corpus Cavernosum Smooth Muscle Cells. Urologia Internationalis 104:7-8, pages 594-603.
Crossref
Paloma A. Harcha, Ximena López, Pablo J. Sáez, Paola Fernández, Iván Barría, Agustín D. Martínez & Juan C. Sáez. (2019) Pannexin-1 Channels Are Essential for Mast Cell Degranulation Triggered During Type I Hypersensitivity Reactions. Frontiers in Immunology 10.
Crossref
Yutong Yang, Xinxin Yan, Jingyi Xue, Yuanyuan Zheng, Min Chen, Zhipeng Sun, Tiantian Liu, Cong Wang, Hongjie You & Dali Luo. (2019) Connexin43 dephosphorylation at serine 282 is associated with connexin43-mediated cardiomyocyte apoptosis. Cell Death & Differentiation 26:7, pages 1332-1345.
Crossref
Raghda Ramadan, Els Vromans, Dornatien Chuo Anang, Elke Decrock, Mohamed Mysara, Pieter Monsieurs, Sarah Baatout, Luc Leybaert & An Aerts. (2019) Single and fractionated ionizing radiation induce alterations in endothelial connexin expression and channel function. Scientific Reports 9:1.
Crossref
Gonzalo I. Gómez, Romina V. Falcon, Carola J. Maturana, Valeria C. Labra, Nicole Salgado, Consuelo A. Rojas, Juan E. Oyarzun, Waldo Cerpa, Rodrigo A. Quintanilla & Juan A. Orellana. (2018) Heavy Alcohol Exposure Activates Astroglial Hemichannels and Pannexons in the Hippocampus of Adolescent Rats: Effects on Neuroinflammation and Astrocyte Arborization. Frontiers in Cellular Neuroscience 12.
Crossref
J. Cheng, G. Jiang, R. Tarzemany, H. Larjava & L. Häkkinen. (2018) Regulation of connexin 43 expression in human gingival fibroblasts. Experimental Cell Research 371:1, pages 238-249.
Crossref
Rana Tarzemany, Guoqiao Jiang, Jean X. Jiang, Corrie Gallant-Behm, Colin Wiebe, David A. Hart, Hannu Larjava & Lari Häkkinen. (2018) Connexin 43 regulates the expression of wound healing-related genes in human gingival and skin fibroblasts. Experimental Cell Research 367:2, pages 150-161.
Crossref
Gonzalo Gómez, Paola Fernández, Victoria Velarde & Juan Sáez. (2018) Angiotensin II-Induced Mesangial Cell Damage Is Preceded by Cell Membrane Permeabilization Due to Upregulation of Non-Selective Channels. International Journal of Molecular Sciences 19:4, pages 957.
Crossref
Rana Tarzemany, Guoqiao Jiang, Jean X. Jiang, Hannu Larjava & Lari Häkkinen. (2017) Connexin 43 Hemichannels Regulate the Expression of Wound Healing-Associated Genes in Human Gingival Fibroblasts. Scientific Reports 7:1.
Crossref
Brian Skriver Nielsen, Daniel Bloch Hansen, Bruce R. Ransom, Morten Schak Nielsen & Nanna MacAulay. (2017) Connexin Hemichannels in Astrocytes: An Assessment of Controversies Regarding Their Functional Characteristics. Neurochemical Research 42:9, pages 2537-2550.
Crossref
Anibal A. Vargas, Bruno A. Cisterna, Fujiko Saavedra-Leiva, Carolina Urrutia, Luis A. Cea, Alex H. Vielma, Sebastian E. Gutierrez-Maldonado, Alberto J. M. Martin, Claudia Pareja-Barrueto, Yerko Escalona, Oliver Schmachtenberg, Carlos F. Lagos, Tomas Perez-Acle & Juan C. Sáez. (2017) On Biophysical Properties and Sensitivity to Gap Junction Blockers of Connexin 39 Hemichannels Expressed in HeLa Cells. Frontiers in Physiology 8.
Crossref
Carlos Puebla, Bruno A. Cisterna, Daniela P. Salas, Fernando Delgado-López, Paul D. Lampe & Juan C. Sáez. (2016) Linoleic acid permeabilizes gastric epithelial cells by increasing connexin 43 levels in the cell membrane via a GPR40- and Akt-dependent mechanism. Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids 1861:5, pages 439-448.
Crossref
Christian C. Naus, Qurratulain Aftab & Wun Chey Sin. (2016) Common mechanisms linking connexin43 to neural progenitor cell migration and glioma invasion. Seminars in Cell & Developmental Biology 50, pages 59-66.
Crossref
Beatriz C. Avendaño, Trinidad D. Montero, Carolina E. Chávez, Rommy von Bernhardi & Juan A. Orellana. (2015) Prenatal exposure to inflammatory conditions increases Cx43 and Panx1 unopposed channel opening and activation of astrocytes in the offspring effect on neuronal survival. Glia 63:11, pages 2058-2072.
Crossref
Rocío Talaverón, Paola Fernández, Rosalba Escamilla, Angel M. Pastor, Esperanza R. Matarredona & Juan C. Sáez. (2015) Neural progenitor cells isolated from the subventricular zone present hemichannel activity and form functional gap junctions with glial cells. Frontiers in Cellular Neuroscience 9.
Crossref
Rainer Schulz, Philipp Maximilian Görge, Anikó Görbe, Péter Ferdinandy, Paul D. Lampe & Luc Leybaert. (2015) Connexin 43 is an emerging therapeutic target in ischemia/reperfusion injury, cardioprotection and neuroprotection. Pharmacology & Therapeutics 153, pages 90-106.
Crossref
Juan C. Sáez, Bruno A. Cisterna, Anibal Vargas & Christopher P. Cardozo. (2015) Regulation of pannexin and connexin channels and their functional role in skeletal muscles. Cellular and Molecular Life Sciences 72:15, pages 2929-2935.
Crossref
Mauricio A. Retamal, Edison P. Reyes, Isaac E. García, Bernardo Pinto, Agustín D. Martínez & Carlos González. (2015) Diseases associated with leaky hemichannels. Frontiers in Cellular Neuroscience 9.
Crossref
Daniela Salas, Carlos Puebla, Paul D. Lampe, Sergio Lavandero & Juan C. S?ez. (2015) Role of Akt and Ca2+ on cell permeabilization via connexin43 hemichannels induced by metabolic inhibition. Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease 1852:7, pages 1268-1277.
Crossref
Manuel A. Riquelme, Luis A. Cea, Jos?? L. Vega, Carlos Puebla, An??bal A. Vargas, Kenji F. Shoji, Mario Subiabre & Juan C. S??ez. (2015) Pannexin channels mediate the acquisition of myogenic commitment in C2C12 reserve cells promoted by P2 receptor activation. Frontiers in Cell and Developmental Biology 3.
Crossref
Juan A. Orellana, Rodrigo Moraga-Amaro, Ra??l D??az-Galarce, Sebasti??n Rojas, Carola J. Maturana, Jimmy Stehberg & Juan C. S??ez. (2015) Restraint stress increases hemichannel activity in hippocampal glial cells and neurons. Frontiers in Cellular Neuroscience 9.
Crossref
Juan A. Orellana, Dolores Busso, Gigliola Ramírez, Marlys Campos, Attilio Rigotti, Jaime Eugenín & Rommy von Bernhardi. (2014) Prenatal nicotine exposure enhances Cx43 and Panx1 unopposed channel activity in brain cells of adult offspring mice fed a high-fat/cholesterol diet. Frontiers in Cellular Neuroscience 8.
Crossref
Giselle Cheung, Oana Chever & Nathalie Rouach. (2014) Connexons and pannexons: newcomers in neurophysiology. Frontiers in Cellular Neuroscience 8.
Crossref
Kurt A. Schalper, Daniel Carvajal-Hausdorf & Mauricio P. Oyarzo. (2014) Possible role of hemichannels in cancer. Frontiers in Physiology 5.
Crossref
Pablo J. Sáez, Kenji F. Shoji, Adam Aguirre & Juan C. Sáez. (2014) Regulation of Hemichannels and Gap Junction Channels by Cytokines in Antigen-Presenting Cells. Mediators of Inflammation 2014, pages 1-23.
Crossref
Manuel A. Riquelme, Luis A. Cea, José L. Vega, Mauricio P. Boric, Hannah Monyer, Michael V.L. Bennett, Marina Frank, Klaus Willecke & Juan C. Sáez. (2013) The ATP required for potentiation of skeletal muscle contraction is released via pannexin hemichannels. Neuropharmacology 75, pages 594-603.
Crossref
Manuel A. Riquelme, Rekha Kar, Sumin Gu & Jean X. Jiang. (2013) Antibodies targeting extracellular domain of connexins for studies of hemichannels. Neuropharmacology 75, pages 525-532.
Crossref
Juan A. Orellana, Trinidad D. Montero & Rommy von Bernhardi. (2013) Astrocytes inhibit nitric oxide-dependent Ca 2+ dynamics in activated microglia: Involvement of ATP released via pannexin 1 channels . Glia 61:12, pages 2023-2037.
Crossref
Roberto Berra-Romani, José Everardo Avelino-Cruz, Abdul Raqeeb, Alessandro Della Corte, Mariapia Cinelli, Stefania Montagnani, Germano Guerra, Francesco Moccia & Franco Tanzi. (2013) Ca2+-dependent nitric oxide release in the injured endothelium of excised rat aorta: a promising mechanism applying in vascular prosthetic devices in aging patients. BMC Surgery 13:S2.
Crossref
Pablo J. Sáez, Juan A. Orellana, Natalia Vega-Riveros, Vania A. Figueroa, Diego E. Hernández, Juan F. Castro, Andrés D. Klein, Jean X. Jiang, Silvana Zanlungo & Juan C. Sáez. (2013) Disruption in Connexin-Based Communication Is Associated with Intracellular Ca2+ Signal Alterations in Astrocytes from Niemann-Pick Type C Mice. PLoS ONE 8:8, pages e71361.
Crossref
Ernesto Oviedo-Orta, Brenda Kwak & William EvansPablo Sáez, Kenji Shoji & Juan Sáez. 2013. Connexin Cell Communication Channels. Connexin Cell Communication Channels 61 88 .
Vania Figueroa, Pablo J. S?ez, Jos? D. Salas, Daniela Salas, Oscar Jara, Agust?n D. Mart?nez, Juan C. S?ez & Mauricio A. Retamal. (2013) Linoleic acid induces opening of connexin26 hemichannels through a PI3K/Akt/Ca2+-dependent pathway. Biochimica et Biophysica Acta (BBA) - Biomembranes 1828:3, pages 1169-1179.
Crossref
Nan Wang, Marijke De Bock, Elke Decrock, Mélissa Bol, Ashish Gadicherla, Mathieu Vinken, Vera Rogiers, Feliksas F. Bukauskas, Geert Bultynck & Luc Leybaert. (2013) Paracrine signaling through plasma membrane hemichannels. Biochimica et Biophysica Acta (BBA) - Biomembranes 1828:1, pages 35-50.
Crossref
Rodrigo A. Quintanilla, Juan A. Orellana & Rommy von Bernhardi. (2012) Understanding Risk Factors for Alzheimer's Disease: Interplay of Neuroinflammation, Connexin-based Communication and Oxidative Stress. Archives of Medical Research 43:8, pages 632-644.
Crossref
Mathieu Vinken, Elke Decrock, Luc Leybaert, Geert Bultynck, Bernard Himpens, Tamara Vanhaecke & Vera Rogiers. (2012) Non-channel functions of connexins in cell growth and cell death. Biochimica et Biophysica Acta (BBA) - Biomembranes 1818:8, pages 2002-2008.
Crossref
Eliana Scemes. (2012) Nature of plasmalemmal functional “hemichannels”. Biochimica et Biophysica Acta (BBA) - Biomembranes 1818:8, pages 1880-1883.
Crossref
Luis A. Cea, Manuel A. Riquelme, Bruno A. Cisterna, Carlos Puebla, José L. Vega, Maximiliano Rovegno & Juan C. Sáez. (2012) Connexin- and Pannexin-Based Channels in Normal Skeletal Muscles and Their Possible Role in Muscle Atrophy. The Journal of Membrane Biology 245:8, pages 423-436.
Crossref
Stefanie GödeckeClaudia Roderigo, Christine R. Rose, Bernhard H. Rauch, Axel GödeckeJürgen Schrader. (2012) Thrombin-induced ATP release from human umbilical vein endothelial cells. American Journal of Physiology-Cell Physiology 302:6, pages C915-C923.
Crossref
Roberto Berra-Romani, Abdul Raqeeb, Julián Torres-Jácome, Alejandro Guzman-Silva, Germano Guerra, Franco Tanzi & Francesco Moccia. (2012) The Mechanism of Injury-Induced Intracellular Calcium Concentration Oscillations in the Endothelium of Excised Rat Aorta. Journal of Vascular Research 49:1, pages 65-76.
Crossref
Juan A. Orellana, Pablo J. Sáez, Christian Cortés-campos, Roberto J. Elizondo, Kenji F. Shoji, Susana Contreras-Duarte, Vania Figueroa, Victoria Velarde, Jean X. Jiang, Francisco Nualart, Juan C. Sáez & María A. García. (2012) Glucose increases intracellular free Ca2+ in tanycytes via ATP released through connexin 43 hemichannels. Glia 60:1, pages 53-68.
Crossref
Liesbeth Ceelen, Freddy Haesebrouck, Tamara Vanhaecke, Vera Rogiers & Mathieu Vinken. (2011) Modulation of connexin signaling by bacterial pathogens and their toxins. Cellular and Molecular Life Sciences 68:18, pages 3047-3064.
Crossref
Juan A. Orellana, Emilio Díaz, Kurt A. Schalper, Aníbal A. Vargas, Michael V.L. Bennett & Juan C. Sáez. (2011) Cation permeation through connexin 43 hemichannels is cooperative, competitive and saturable with parameters depending on the permeant species. Biochemical and Biophysical Research Communications 409:4, pages 603-609.
Crossref
Simone Pollok, Ann-Catherine Pfeiffer, Ralf Lobmann, Catherine S. Wright, Ingrid Moll, Patricia E. M. Martin & Johanna M. Brandner. (2011) Connexin 43 mimetic peptide Gap27 reveals potential differences in the role of Cx43 in wound repair between diabetic and non-diabetic cells. Journal of Cellular and Molecular Medicine 15:4, pages 861-873.
Crossref
Kaijun Luo & Matthew W. Turnbull. (2011) Characterization of Nonjunctional Hemichannels in Caterpillar Cells. Journal of Insect Science 11:6, pages 1-17.
Crossref
Mathieu Vinken, Elke Decrock, Elke De Vuyst, Raf Ponsaerts, Catheleyne D'hondt, Geert Bultynck, Liesbeth Ceelen, Tamara Vanhaecke, Luc Leybaert & Vera Rogiers. (2011) Connexins: sensors and regulators of cell cycling. Biochimica et Biophysica Acta (BBA) - Reviews on Cancer 1815:1, pages 13-25.
Crossref
Kurt A. SchalperHelmuth A. SánchezSung C. LeeGuillermo A. AltenbergMichael H. Nathanson & Juan C. Sáez. (2010) Connexin 43 hemichannels mediate the Ca 2+ influx induced by extracellular alkalinization . American Journal of Physiology-Cell Physiology 299:6, pages C1504-C1515.
Crossref
Jinny J. Yoon, Colin R. Green, Simon J. O’Carroll & Louise F.B. Nicholson. (2010) Dose-dependent protective effect of connexin43 mimetic peptide against neurodegeneration in an ex vivo model of epileptiform lesion. Epilepsy Research 92:2-3, pages 153-162.
Crossref
Mathieu Vinken, Elke Decrock, Elke De Vuyst, Marijke De Bock, Roosmarijn E. Vandenbroucke, Bruno G. De Geest, Joseph Demeester, Niek N. Sanders, Tamara Vanhaecke, Luc Leybaert & Vera Rogiers. (2009) Connexin32 hemichannels contribute to the apoptotic-to-necrotic transition during Fas-mediated hepatocyte cell death. Cellular and Molecular Life Sciences 67:6, pages 907-918.
Crossref
Mauricio A. Retamal, ShengYong Yin, Guillermo A. Altenberg & Luis Reuss. (2010) Voltage-dependent facilitation of Cx46 hemichannels. American Journal of Physiology-Cell Physiology 298:1, pages C132-C139.
Crossref
B.D. Kalmatsky, S. Bhagan, Q. Tang, T.A. Bargiello & T.L. Dowd. (2009) Structural studies of the N-terminus of Connexin 32 using 1H NMR spectroscopy. Archives of Biochemistry and Biophysics 490:1, pages 9-16.
Crossref
Russell D. Salter & Simon C. Watkins. (2009) Dendritic cell altered states: what role for calcium?. Immunological Reviews 231:1, pages 278-288.
Crossref
Catheleyne D'hondt, Raf Ponsaerts, Humbert De Smedt, Geert Bultynck & Bernard Himpens. (2009) Pannexins, distant relatives of the connexin family with specific cellular functions?. BioEssays 31:9, pages 953-974.
Crossref
Mauricio A. Retamal, ShengYong Yin, Guillermo A. Altenberg & Luis Reuss. (2009) Modulation of Cx46 hemichannels by nitric oxide. American Journal of Physiology-Cell Physiology 296:6, pages C1356-C1363.
Crossref
Eliana Scemes, David C. Spray & Paolo Meda. (2008) Connexins, pannexins, innexins: novel roles of “hemi-channels”. Pflügers Archiv - European Journal of Physiology 457:6, pages 1207-1226.
Crossref
George R. Dubyak. (2009) Both sides now: multiple interactions of ATP with pannexin-1 hemichannels . Focus on “A permeant regulating its permeation pore: inhibition of pannexin 1 channels by ATP” . American Journal of Physiology-Cell Physiology 296:2, pages C235-C241.
Crossref
Roman A. Romanov, Olga A. Rogachevskaja, Alexander A. Khokhlov & Stanislav S. Kolesnikov. (2008) Voltage Dependence of ATP Secretion in Mammalian Taste Cells. The Journal of General Physiology 132:6, pages 731-744.
Crossref

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Order Reprints Request Corporate Permissions

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

Request Academic Permissions

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.

Related research

People also read lists articles that other readers of this article have read.

Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.

Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.