3,984
Views
191
CrossRef citations to date
0
Altmetric
Review

Development of 2A peptide-based strategies in the design of multicistronic vectors

&
Pages 627-638 | Published online: 24 Nov 2005

Keep up to date with the latest research on this topic with citation updates for this article.

Read on this site (2)

Jing Chen & Jianping Xie. (2012) Progress on RNAi-based molecular medicines. International Journal of Nanomedicine 7, pages 3971-3980.
Read now
Magnus Blø, David R Micklem & James B Lorens. (2007) Drug target discovery using retroviruses. Expert Opinion on Drug Discovery 2:10, pages 1285-1300.
Read now

Articles from other publishers (189)

Yi-Qun Gao, Jin-Quan Huang, Guilhem Reyt, Tao Song, Ashley LoveDavid TiemessenPei-Ying Xue, Wen-Kai Wu, Michael W. George, Xiao-Ya Chen, Dai-Yin Chao, Gabriel Castrillo & David E. Salt. (2023) A dirigent protein complex directs lignin polymerization and assembly of the root diffusion barrier. Science 382:6669, pages 464-471.
Crossref
Yuko Watanabe, Eri Katsumura, Tatsuki Domon, Yuta Ishikawa, Rina Oguri, Minami Takashima, Qi Meng, Masato Kinoshita, Hisashi Hashimoto & Kiyotaka Hitomi. (2023) Establishment of transgenic epithelium-specific Cre-recombinase driving medaka ( Oryzias latipes ) by homology repair mediated knock-in . Bioscience, Biotechnology, and Biochemistry 87:11, pages 1285-1294.
Crossref
Juanjuan Long, Andrea Mariossi, Chen Cao, Zhongying Mo, Joel W. Thompson, Michael S. Levine & Laurence A. Lemaire. (2023) Cereblon influences the timing of muscle differentiation in Ciona tadpoles . Proceedings of the National Academy of Sciences 120:43.
Crossref
Beverly Naigles, Avaneesh V. Narla, Jan Soroczynski, Lev S. Tsimring & Nan Hao. (2023) Quantifying dynamic pro-inflammatory gene expression and heterogeneity in single macrophage cells. Journal of Biological Chemistry 299:10, pages 105230.
Crossref
Audrey Page, Marie Delles, Didier Nègre, Caroline Costa, Floriane Fusil & François-Loïc Cosset. (2023) Engineering B cells with customized therapeutic responses using a synthetic circuit. Molecular Therapy - Nucleic Acids 33, pages 1-14.
Crossref
Habib Bouguenina, Stephanos Nicolaou, Yann-Vaï Le Bihan, Elizabeth A. Bowling, Cheyenne Calderon, John J. Caldwell, Brinley Harrington, Angela Hayes, P. Craig McAndrew, Costas Mitsopoulos, Fernando Jr. Sialana, Andrea Scarpino, Mark Stubbs, Arjun Thapaliya, Siddhartha Tyagi, Hannah Z. Wang, Francesca Wood, Rosemary Burke, Florence Raynaud, Jyoti Choudhary, Rob L.M. van Montfort, Amine Sadok, Thomas F. Westbrook, Ian Collins & Rajesh Chopra. (2023) iTAG an optimized IMiD-induced degron for targeted protein degradation in human and murine cells. iScience 26:7, pages 107059.
Crossref
Emmanuelle Totain, Loïc Lindner, Nicolas Martin, Yolande Misseri, Alexandra Iché, Marie-Christine Birling, Tania Sorg, Yann Herault, Alain Bousquet-Melou, Pascale Bouillé, Christine Duthoit, Guillaume Pavlovic & Severine Boullier. (2023) Development of HPV16 mouse and dog models for more accurate prediction of human vaccine efficacy. Laboratory Animal Research 39:1.
Crossref
Paul J. Russell, Jacob A. Slivka, Elaina P. Boyle, Arthur H.M. Burghes & Michael G. Kearse. (2023) Translation reinitiation after uORFs does not fully protect mRNAs from nonsense-mediated decay. RNA 29:6, pages 735-744.
Crossref
Brooke Bollman, Naveen Nunna, Kapil Bahl, Chiaowen Joyce Hsiao, Hamilton Bennett, Scott Butler, Bryant Foreman, Katherine E. Burgomaster, Maya Aleshnick, Wing-Pui Kong, Brian E. Fisher, Tracy J. Ruckwardt, Kaitlyn M. Morabito, Barney S. Graham, Kimberly A. Dowd, Theodore C. Pierson & Andrea Carfi. (2023) An optimized messenger RNA vaccine candidate protects non-human primates from Zika virus infection. npj Vaccines 8:1.
Crossref
Minyang Wang, Yimin Shang, Xiaomeng Liu & Sanfeng Chen. (2023) Assembly of nitrogenase biosynthetic pathway in Saccharomyces cerevisiae by using polyprotein strategy. Frontiers in Microbiology 14.
Crossref
Yayi Gao & Rémy Bosselut. 2023. T-Cell Development. T-Cell Development 199 209 .
Marc Dalod & Stefanie Scheu. (2022) Dendritic cell functions in vivo: A user's guide to current and next‐ generation mutant mouse models. European Journal of Immunology 52:11, pages 1712-1749.
Crossref
Enyue Fang, Xiaohui Liu, Miao Li, Jingjing Liu, Zelun Zhang, Xinyu Liu, Xingxing Li, Wenjuan Li, Qinhua Peng, Yongxin Yu & Yuhua Li. (2022) Construction of a Dengue NanoLuc Reporter Virus for In Vivo Live Imaging in Mice. Viruses 14:6, pages 1253.
Crossref
Hanieh Moradian, Manfred Gossen & Andreas Lendlein. (2022) Co-delivery of genes can be confounded by bicistronic vector design. MRS Communications 12:2, pages 145-153.
Crossref
Anna Georgina Kopasz, Dávid Zsolt Pusztai, Réka Karkas, Liza Hudoba, Khaldoon Sadiq Ahmed Abdullah, Gergely Imre, Gabriella Pankotai-Bodó, Ede Migh, Andrea Nagy, András Kriston, Péter Germán, Andrea Bakné Drubi, Anna Molnár, Ildikó Fekete, Virág Éva Dani, Imre Ocsovszki, László Géza Puskás, Péter Horváth, Farkas Sükösd & Lajos Mátés. (2022) A versatile transposon-based technology to generate loss- and gain-of-function phenotypes in the mouse liver. BMC Biology 20:1.
Crossref
Nuwan C. Hettige, Huashan Peng, Hanrong Wu, Xin Zhang, Volodymyr Yerko, Ying Zhang, Malvin Jefri, Vincent Soubannier, Gilles Maussion, Shaima Alsuwaidi, Anjie Ni, Cecilia Rocha, Jeyashree Krishnan, Vincent McCarty, Lilit Antonyan, Andreas Schuppert, Gustavo Turecki, Edward A. Fon, Thomas M. Durcan & Carl Ernst. (2022) FOXG1 dose tunes cell proliferation dynamics in human forebrain progenitor cells. Stem Cell Reports 17:3, pages 475-488.
Crossref
Yanyan Wang, Xiaowei Li, Xin Chen, Jens Nielsen, Dina Petranovic & Verena Siewers. (2021) Expression of antibody fragments in Saccharomyces cerevisiae strains evolved for enhanced protein secretion. Microbial Cell Factories 20:1.
Crossref
Xuekun Wang & Mario Andrea Marchisio. (2021) Synthetic polycistronic sequences in eukaryotes. Synthetic and Systems Biotechnology 6:4, pages 254-261.
Crossref
Ningning Tang, Ling Li, Fei Xie, Ying Lu, Zifan Zuo, Hao Shan, Quan Zhang & Lianwen Zhang. (2021) A living cell-based fluorescent reporter for high-throughput screening of anti-tumor drugs. Journal of Pharmaceutical Analysis 11:6, pages 808-814.
Crossref
Amalia Riga, Janine Cravo, Ruben Schmidt, Helena R. Pires, Victoria G. Castiglioni, Sander van den Heuvel & Mike Boxem. (2021) Caenorhabditis elegans LET-413 Scribble is essential in the epidermis for growth, viability, and directional outgrowth of epithelial seam cells. PLOS Genetics 17:10, pages e1009856.
Crossref
Lawrence P. Andrews, Kate M. Vignali, Andrea L. Szymczak-Workman, Amanda R. Burton, Erin A. Brunazzi, Shin Foong Ngiow, Akihito Harusato, Arlene H. Sharpe, E. John Wherry, Ichiro Taniuchi, Creg J. Workman & Dario A.A. Vignali. (2021) A Cre-driven allele-conditioning line to interrogate CD4+ conventional T cells. Immunity 54:10, pages 2209-2217.e6.
Crossref
Hannes Schihada, Rawan Shekhani & Gunnar Schulte. (2021) Quantitative assessment of constitutive G protein–coupled receptor activity with BRET-based G protein biosensors. Science Signaling 14:699.
Crossref
Tetsuichi Wazawa, Ryohei Noma, Shusaku Uto, Kazunori Sugiura, Takashi Washio & Takeharu Nagai. (2021) A photoswitchable fluorescent protein for hours-time-lapse and sub-second-resolved super-resolution imaging. Microscopy 70:4, pages 340-352.
Crossref
Daniel J. Perry, Leeana D. Peters, Priya Saikumar Lakshmi, Lin Zhang, Zhao Han, Clive H. Wasserfall, Clayton E. Mathews, Mark A. Atkinson & Todd M. Brusko. (2021) Overexpression of the PTPN22 Autoimmune Risk Variant LYP-620W Fails to Restrain Human CD4+ T Cell Activation . The Journal of Immunology 207:3, pages 849-859.
Crossref
Youming Li, Da-Quan Jiang & Chen Jia. (2021) Steady-state joint distribution for first-order stochastic reaction kinetics. Physical Review E 104:2.
Crossref
Martín Habif, Agustín A. Corbat, Mauro Silberberg & Hernán E. Grecco. (2021) CASPAM: A Triple-Modality Biosensor for Multiplexed Imaging of Caspase Network Activity. ACS Sensors 6:7, pages 2642-2653.
Crossref
Wenni You, Mengjing Li, Yilin Qi, Yanbing Wang, Yiwu Chen, Ying Liu, Li Li, Hongsheng Ouyang & Daxin Pang. (2021) CRISPR/Cas9-Mediated Specific Integration of Fat-1 and IGF-1 at the pRosa26 Locus. Genes 12:7, pages 1027.
Crossref
Chenxu Guo, Francis K. Fordjour, Shang Jui Tsai, James C. Morrell & Stephen J. Gould. (2021) Choice of selectable marker affects recombinant protein expression in cells and exosomes. Journal of Biological Chemistry 297:1, pages 100838.
Crossref
Robert J. Mallis, Jonathan S. Duke-Cohan, Dibyendu Kumar DasAoi AkitsuAdrienne M. LuomaDebasis Banik, Hannah M. Stephens, Paul W. Tetteh, Caitlin D. CastroSophie KrahnkeRebecca E. Hussey, Brian LawneyKristine N. BrazinPedro A. Reche, Wonmuk Hwang, Erin J. Adams, Matthew J. Lang & Ellis L. Reinherz. (2021) Molecular design of the γδT cell receptor ectodomain encodes biologically fit ligand recognition in the absence of mechanosensing. Proceedings of the National Academy of Sciences 118:26.
Crossref
Yang Zhou, Meiqi Ding, Xiaodong Duan, Kai R. Konrad, Georg Nagel & Shiqiang Gao. (2021) Extending the Anion Channelrhodopsin-Based Toolbox for Plant Optogenetics. Membranes 11:4, pages 287.
Crossref
Riona Hatazawa, Saori Fukuda, Kanako Kumamoto, Fumio Matsushita, Shizuko Nagao, Takayuki Murata, Koki Taniguchi, Taei Matsui & Satoshi Komoto. (2021) Strategy for generation of replication–competent recombinant rotaviruses expressing multiple foreign genes. Journal of General Virology 102:4.
Crossref
Jeremy J. Work & Onn Brandman. (2021) Adaptability of the ubiquitin-proteasome system to proteolytic and folding stressors. Journal of Cell Biology 220:3.
Crossref
RANJANI RAJASEKARAN, J JOHN KIRUBAHARAN, P SHILPA, M VIDHYA & S RAJALAKSHMI. (2021) Viral 2A-peptides mediate continuous transcription and self-cleavage of multiple heterologous genes in fowlpox virus vector. The Indian Journal of Animal Sciences 90:9, pages 1234-1239.
Crossref
Ulfert Rand, Tobias Kubsch, Bahram Kasmapour & Luka Cicin-Sain. (2021) A Novel Triple-Fluorescent HCMV Strain Reveals Gene Expression Dynamics and Anti-Herpesviral Drug Mechanisms. Frontiers in Cellular and Infection Microbiology 10.
Crossref
Scott K. Lyons, Kevin P. Francis & Christopher H. Contag. 2021. Molecular Imaging. Molecular Imaging 113 141 .
Yunlong Jia, Françoise Bleicher, Jonathan Reboulet & Samir Merabet. 2021. Multiplexed Imaging. Multiplexed Imaging 173 190 .
Naeramit Sontayananon, Charles Redwood, Benjamin Davies & Katja Gehmlich. (2020) Fluorescent PSC-Derived Cardiomyocyte Reporter Lines: Generation Approaches and Their Applications in Cardiovascular Medicine. Biology 9:11, pages 402.
Crossref
Kai A. Kropp, Alberto Domingo López-Muñoz, Birgit Ritter, Rocío Martín, Alberto Rastrojo, Sangar Srivaratharajan, Katinka Döhner, Akshay Dhingra, Julia S. Czechowicz, Claus-Henning Nagel, Beate Sodeik, Antonio Alcami & Abel Viejo-Borbolla. (2020) Herpes Simplex Virus 2 Counteracts Neurite Outgrowth Repulsion during Infection in a Nerve Growth Factor-Dependent Manner. Journal of Virology 94:20.
Crossref
Jasper Che-Yung Chien, Elie Tabet, Kelsey Pinkham, Cintia Carla da Hora, Jason Cheng-Yu Chang, Steven Lin, Christian E Badr & Charles Pin-Kuang Lai. (2020) A multiplexed bioluminescent reporter for sensitive and non-invasive tracking of DNA double strand break repair dynamics in vitro and in vivo. Nucleic Acids Research 48:17, pages e100-e100.
Crossref
Aashima Khosla, Cecilia Rodriguez‐Furlan, Suraj Kapoor, Jaimie M. Van Norman & David C. Nelson. (2020) A series of dual‐reporter vectors for ratiometric analysis of protein abundance in plants. Plant Direct 4:6.
Crossref
Audrey Page, Floriane Fusil & François-Loïc Cosset. (2020) Towards Physiologically and Tightly Regulated Vectored Antibody Therapies. Cancers 12:4, pages 962.
Crossref
Xi‐bin Lu, Yu‐han Guo & Wei Huang. (2020) Characterization of the cHS4 insulator in mouse embryonic stem cells. FEBS Open Bio 10:4, pages 644-656.
Crossref
Timo Schomann, Laura Mezzanotte, John C. M. J. de Groot, Clemens W. G. M. Löwik, Johan H. M. Frijns & Margriet A. Huisman. (2019) Imaging Bioluminescent Exogenous Stem Cells in the Intact Guinea Pig Cochlea. The Anatomical Record 303:3, pages 427-440.
Crossref
Nicola Mitwasi, Anja Feldmann, Claudia Arndt, Stefanie Koristka, Nicole Berndt, Justyna Jureczek, Liliana R. Loureiro, Ralf Bergmann, Domokos Máthé, Nikolett Hegedüs, Tibor Kovács, Congcong Zhang, Pranav Oberoi, Elke Jäger, Barbara Seliger, Claudia Rössig, Achim Temme, Jiri Eitler, Torsten Tonn, Marc Schmitz, Jessica C. Hassel, Dirk Jäger, Winfried S. Wels & Michael Bachmann. (2020) “UniCAR”-modified off-the-shelf NK-92 cells for targeting of GD2-expressing tumour cells. Scientific Reports 10:1.
Crossref
Hidetaka Kioka, Hisakazu Kato, Takeshi Fujita, Yoshihiro Asano, Yasunori Shintani, Satoru Yamazaki, Osamu Tsukamoto, Hiromi Imamura, Mikihiko Kogo, Masafumi Kitakaze, Yasushi Sakata & Seiji Takashima. (2020) In vivo real‐time ATP imaging in zebrafish hearts reveals G0s2 induces ischemic tolerance. The FASEB Journal 34:2, pages 2041-2054.
Crossref
Cole S. Sitron, Joseph H. Park, Jenna M. Giafaglione & Onn Brandman. (2020) Aggregation of CAT tails blocks their degradation and causes proteotoxicity in S. cerevisiae. PLOS ONE 15:1, pages e0227841.
Crossref
Andrea Sorrentino, Emrah Eroglu & Thomas Michel. 2020. Oxidative Stress. Oxidative Stress 97 112 .
Paola Sette, Nduka Amankulor, Aofei Li, Marco Marzulli, Daniela Leronni, Mingdi Zhang, William F. Goins, Balveen Kaur, Chelsea Bolyard, Timothy P. Cripe, Jianhua Yu, E. Antonio Chiocca, Joseph C. Glorioso & Paola Grandi. (2019) GBM-Targeted oHSV Armed with Matrix Metalloproteinase 9 Enhances Anti-tumor Activity and Animal Survival. Molecular Therapy - Oncolytics 15, pages 214-222.
Crossref
Kyuri Lee, Soo Young Kim, Yunmi Seo, Hyokyung Kwon, Young Jik Kwon & Hyukjin Lee. (2019) Multicistronic IVT mRNA for simultaneous expression of multiple fluorescent proteins. Journal of Industrial and Engineering Chemistry 80, pages 770-777.
Crossref
João Pedro Fonseca, Alain R. Bonny, G. Renuka Kumar, Andrew H. Ng, Jason Town, Qiu Chang Wu, Elham Aslankoohi, Susan Y. Chen, Galen Dods, Patrick Harrigan, Lindsey C. Osimiri, Amy L. Kistler & Hana El-Samad. (2019) A Toolkit for Rapid Modular Construction of Biological Circuits in Mammalian Cells. ACS Synthetic Biology 8:11, pages 2593-2606.
Crossref
Alisa A. Shaimardanova, Kristina V. Kitaeva, Ilmira I. Abdrakhmanova, Vladislav M. Chernov, Catrin S. Rutland, Albert A. Rizvanov, Daria S. Chulpanova & Valeriya V. Solovyeva. (2019) Production and Application of Multicistronic Constructs for Various Human Disease Therapies. Pharmaceutics 11:11, pages 580.
Crossref
Arash Farhadi, Gabrielle H. Ho, Daniel P. Sawyer, Raymond W. Bourdeau & Mikhail G. Shapiro. (2019) Ultrasound imaging of gene expression in mammalian cells. Science 365:6460, pages 1469-1475.
Crossref
Julie Goudy, Trevor Henley, Hernán G. Méndez & Michael Bressan. (2019) Simplified platform for mosaic in vivo analysis of cellular maturation in the developing heart. Scientific Reports 9:1.
Crossref
Kumi Morikawa, Kazuomi Nakamura, Yoshiko Suyama, Kenshiro Yamamoto, Kohei Fukuoka, Shunjiro Yagi, Yasuaki Shirayoshi, Tetsuya Ohbayashi & Ichiro Hisatome. (2019) Novel dual-reporter transgenic rodents enable cell tracking in animal models of stem cell transplantation. Biochemistry and Biophysics Reports 18, pages 100645.
Crossref
Mio K. Shibuta, Megumi Matsuoka & Sachihiro Matsunaga. (2019) 2A Peptides Contribute to the Co-Expression of Proteins for Imaging and Genome Editing. CYTOLOGIA 84:2, pages 107-111.
Crossref
Cole S. Sitron & Onn Brandman. (2019) CAT tails drive degradation of stalled polypeptides on and off the ribosome. Nature Structural & Molecular Biology 26:6, pages 450-459.
Crossref
Li He, Richard Binari, Jiuhong Huang, Julia Falo-Sanjuan & Norbert Perrimon. (2019) In vivo study of gene expression with an enhanced dual-color fluorescent transcriptional timer. eLife 8.
Crossref
Manfred Schartl, Susanne Schories, Yuko Wakamatsu, Yusuke Nagao, Hisashi Hashimoto, Chloé Bertin, Brigitte Mourot, Cornelia Schmidt, Dagmar Wilhelm, Lazaro Centanin, Yann Guiguen & Amaury Herpin. (2018) Sox5 is involved in germ-cell regulation and sex determination in medaka following co-option of nested transposable elements. BMC Biology 16:1.
Crossref
Konrad Fischer, Alexander Kind & Angelika Schnieke. (2018) Assembling multiple xenoprotective transgenes in pigs. Xenotransplantation 25:6.
Crossref
Byron H. Hartman, Robert Bӧscke, Daniel C. Ellwanger, Sawa Keymeulen, Mirko Scheibinger & Stefan Heller. (2018) Fbxo2 mouse and embryonic stem cell reporter lines delineate in vitro-generated inner ear sensory epithelia cells and enable otic lineage selection and Cre-recombination. Developmental Biology 443:1, pages 64-77.
Crossref
Theodore Williams, Harsha S. Krovi, Laurie G. Landry, Frances Crawford, Niyun Jin, Anita Hohenstein, Megan E. DeNicola, Aaron W. Michels, Howard W. Davidson, Sally C. Kent, Laurent Gapin, John W. Kappler & Maki Nakayama. (2018) Development of T cell lines sensitive to antigen stimulation. Journal of Immunological Methods 462, pages 65-73.
Crossref
Sudarat Hadpech, Wannarat Jinathep, Somphot Saoin, Weeraya Thongkum, Koollawat Chupradit, Umpa Yasamut, Sutpirat Moonmuang & Chatchai Tayapiwatana. (2018) Impairment of a membrane-targeting protein translated from a downstream gene of a “self-cleaving” T2A peptide conjunction. Protein Expression and Purification 150, pages 17-25.
Crossref
Fang Han, Yoshitaka Miyagawa, Gianluca Verlengia, Selene Ingusci, Marie Soukupova, Michele Simonato, Joseph C. Glorioso & Justus B. Cohen. (2018) Cellular Antisilencing Elements Support Transgene Expression from Herpes Simplex Virus Vectors in the Absence of Immediate Early Gene Expression. Journal of Virology 92:17.
Crossref
Maha Tijani, Altar M. Munis, Christopher Perry, Khaled Sanber, Marta Ferraresso, Tarit Mukhopadhyay, Michael Themis, Ilaria Nisoli, Giada Mattiuzzo, Mary K. Collins & Yasuhiro Takeuchi. (2018) Lentivector Producer Cell Lines with Stably Expressed Vesiculovirus Envelopes. Molecular Therapy - Methods & Clinical Development 10, pages 303-312.
Crossref
M. Nazhif Zaini, Saroor A. Patel, Saiful E. Syafruddin, Paulo Rodrigues & Sakari Vanharanta. (2018) Endogenous HIF2A reporter systems for high-throughput functional screening. Scientific Reports 8:1.
Crossref
Jo-Hao Ho, Edward L. StahlCullen L. SchmidSarah M. Scarry, Jeffrey Aubé & Laura M. Bohn. (2018) G protein signaling–biased agonism at the κ-opioid receptor is maintained in striatal neurons. Science Signaling 11:542.
Crossref
Fengjuan Liu, Jinji Liu, Zaimei Yuan, Yubo Qing, Honghui Li, Kaixiang Xu, Wanyun Zhu, Heng Zhao, Baoyu Jia, Weirong Pan, Jianxiong Guo, Xuezeng Zhang, Wenmin Cheng, Wei Wang, Hong-Ye Zhao & Hong-Jiang Wei. (2018) Generation of GTKO Diannan Miniature Pig Expressing Human Complementary Regulator Proteins hCD55 and hCD59 via T2A Peptide-Based Bicistronic Vectors and SCNT. Molecular Biotechnology 60:8, pages 550-562.
Crossref
Maysam Mansouri & Philipp Berger. (2018) Multigene delivery in mammalian cells: Recent advances and applications. Biotechnology Advances 36:4, pages 871-879.
Crossref
Joan Comenge, Jack Sharkey, Oihane Fragueiro, Bettina Wilm, Mathias Brust, Patricia Murray, Raphael Levy & Antonius Plagge. (2018) Multimodal cell tracking from systemic administration to tumour growth by combining gold nanorods and reporter genes. eLife 7.
Crossref
Bin Gu, Eszter Posfai & Janet Rossant. (2018) Efficient generation of targeted large insertions by microinjection into two-cell-stage mouse embryos. Nature Biotechnology 36:7, pages 632-637.
Crossref
Navin Gupta, Koichiro Susa, Yoko Yoda, Joseph V. Bonventre, M. Todd Valerius & Ryuji Morizane. (2018) CRISPR/Cas9‐based Targeted Genome Editing for the Development of Monogenic Diseases Models with Human Pluripotent Stem Cells. Current Protocols in Stem Cell Biology 45:1.
Crossref
Yi Chen, Tingyu Song, Yong-Le Xiao, Xiaoping Wan, Luyi Yang, Jinghai Li, Guangzhi Zeng, Pengfei Fang, Ze-Zhou Wang & Rong Gao. (2018) Enhancement of immune response of piglets to PCV-2 vaccine by porcine IL-2 and fusion IL-4/6 gene entrapped in chitosan nanoparticles. Research in Veterinary Science 117, pages 224-232.
Crossref
Qi Xiong, Jianlin Chen, Fei-Lin Li, Shiji Zhao, Xiaoping Wan, Xiao Yang, Jianglin Li, Danyu Luo, Zezhou Wang, Xuebin Lv & Rong Gao. (2017) Co-expression of mFat-1 and pig IGF-1 genes by recombinant plasmids in modified chitosan nanoparticles and its synergistic effect on mouse immunity. Scientific Reports 7:1.
Crossref
Matthew L. Bettini, Po-Chein Chou, Clifford S. Guy, Thomas Lee, Kate M. Vignali & Dario A. A. Vignali. (2017) Cutting Edge: CD3 ITAM Diversity Is Required for Optimal TCR Signaling and Thymocyte Development. The Journal of Immunology 199:5, pages 1555-1560.
Crossref
Shinta Saito, Aya Kurosawa & Noritaka Adachi. (2017) Mechanistic basis for increased human gene targeting by promoterless vectors-roles of homology arms and Rad54 paralogs. The FEBS Journal 284:17, pages 2748-2763.
Crossref
Yaron E. Antebi, James M. Linton, Heidi Klumpe, Bogdan Bintu, Mengsha Gong, Christina Su, Reed McCardell & Michael B. Elowitz. (2017) Combinatorial Signal Perception in the BMP Pathway. Cell 170:6, pages 1184-1196.e24.
Crossref
Ziqing Liu, Olivia Chen, J. Blake Joseph Wall, Michael Zheng, Yang Zhou, Li Wang, Haley Ruth Vaseghi, Li Qian & Jiandong Liu. (2017) Systematic comparison of 2A peptides for cloning multi-genes in a polycistronic vector. Scientific Reports 7:1.
Crossref
Erin K. Borchardt, Rita M. Meganck, Heather A. Vincent, Christopher B. Ball, Silvia B.V. Ramos, Nathaniel J. Moorman, William F. Marzluff & Aravind Asokan. (2017) Inducing circular RNA formation using the CRISPR endoribonuclease Csy4. RNA 23:5, pages 619-627.
Crossref
Jing Xu, Wenhua Zhu, Yuanxu Guo, Congshan Jiang, Nazim Hussain, Liesu Meng & Shemin Lu. (2016) Construction of Conveniently Screening pLKO.1-TRC Vector Tagged with TurboGFP. Applied Biochemistry and Biotechnology 181:2, pages 699-709.
Crossref
C. Cavelti‐Weder, A. Zumsteg, W. Li & Q. Zhou. (2017) Reprogramming of Pancreatic Acinar Cells to Functional Beta Cells by In Vivo Transduction of a Polycistronic Construct Containing Pdx1, Ngn3, MafA in Mice. Current Protocols in Stem Cell Biology 40:1.
Crossref
Kirsten Cant?-Barrett, Rui D. Mendes, Willem K. Smits, Yvette M. van Helsdingen-van Wijk, Rob Pieters & Jules P. P. Meijerink. (2016) Lentiviral gene transfer into human and murine hematopoietic stem cells: size matters. BMC Research Notes 9:1.
Crossref
Fei Xu, Xiuhua Kang, Liang Chen, Chuanhui Chen, Gen Hu, Wei Bai & Wei Zhang. (2016) Generation of IL10 and TGFB1 coexpressed mice displaying resistance to ovalbumin-induced asthma. Transgenic Research 25:6, pages 829-837.
Crossref
Tim Kao, Tanya Labonne, Jonathan C. Niclis, Ritu Chaurasia, Zerina Lokmic, Elizabeth Qian, Freya F. Bruveris, Sara E. Howden, Ali Motazedian, Jacqueline V. Schiesser, Magdaline Costa, Koula Sourris, Elizabeth Ng, David Anderson, Antonietta Giudice, Peter Farlie, Michael Cheung, Shireen R. Lamande, Anthony J. Penington, Clare L. Parish, Lachlan H. Thomson, Arash Rafii, David A. Elliott, Andrew G. Elefanty & Edouard G. Stanley. (2016) GAPTrap: A Simple Expression System for Pluripotent Stem Cells and Their Derivatives. Stem Cell Reports 7:3, pages 518-526.
Crossref
K Roellecke, E L Virts, R Einholz, K Z Edson, B Altvater, C Rossig, D von Laer, K Scheckenbach, M Wagenmann, D Reinhardt, C M Kramm, A E Rettie, C Wiek & H Hanenberg. (2016) Optimized human CYP4B1 in combination with the alkylator prodrug 4-ipomeanol serves as a novel suicide gene system for adoptive T-cell therapies. Gene Therapy 23:7, pages 615-626.
Crossref
Young-Hee Jeong, Yeong Ji Kim, Eun Young Kim, Se Eun Kim, Jiwoo Kim, Min Jee Park, Hong-Gu Lee, Se Pill Park & Man-Jong Kang. (2015) Knock-in fibroblasts and transgenic blastocysts for expression of human FGF2 in the bovine β-casein gene locus using CRISPR/Cas9 nuclease-mediated homologous recombination. Zygote 24:3, pages 442-456.
Crossref
Judith Fliegmann, Alain Jauneau, Carole Pichereaux, Charles Rosenberg, Virginie Gasciolli, Antonius C.J. Timmers, Odile Burlet-Schiltz, Julie Cullimore & Jean-Jacques Bono. (2016) LYR3, a high-affinity LCO-binding protein of Medicago truncatula , interacts with LYK3, a key symbiotic receptor . FEBS Letters 590:10, pages 1477-1487.
Crossref
James M. Wagner & Hal S. Alper. (2016) Synthetic biology and molecular genetics in non-conventional yeasts: Current tools and future advances. Fungal Genetics and Biology 89, pages 126-136.
Crossref
Shinta Saito & Noritaka Adachi. (2016) Advances in the Development of Gene-Targeting Vectors to Increase the Efficiency of Genetic Modification. Biological & Pharmaceutical Bulletin Biological and Pharmaceutical Bulletin 39:1, pages 25-32.
Crossref
Jaemi S. Chu, Daniel O. Villarreal & David B. Weiner. 2016. Vaccine Design. Vaccine Design 355 361 .
Shinta Saito, Kiyoe Ura, Miho Kodama & Noritaka Adachi. (2015) Construction and applications of exon-trapping gene-targeting vectors with a novel strategy for negative selection. BMC Research Notes 8:1.
Crossref
Sebastian Brennig, Nico Lachmann, Theresa Buchegger, Miriam Hetzel, Axel Schambach & Thomas Moritz. (2015) Chemoprotection of murine hematopoietic cells by combined gene transfer of cytidine deaminase (CDD) and multidrug resistance 1 gene (MDR1). Journal of Experimental & Clinical Cancer Research 34:1.
Crossref
Jo E. Lewis, John M. Brameld, Phil Hill, Perry Barrett, Francis J.P. Ebling & Preeti H. Jethwa. (2015) The use of a viral 2A sequence for the simultaneous over-expression of both the vgf gene and enhanced green fluorescent protein (eGFP) in vitro and in vivo. Journal of Neuroscience Methods 256, pages 22-29.
Crossref
Hongsuk Park, Sungpil Cho, Margit M. Janat-Amsbury & You Han Bae. (2015) Enhanced thermogenic program by non-viral delivery of combinatory browning genes to treat diet-induced obesity in mice. Biomaterials 73, pages 32-41.
Crossref
Mingru Yin, Weihua Jiang, Zhenfu Fang, Pengcheng Kong, Fengying Xing, Yao Li, Xuejin Chen & Shangang Li. (2015) Generation of hypoxanthine phosphoribosyltransferase gene knockout rabbits by homologous recombination and gene trapping through somatic cell nuclear transfer. Scientific Reports 5:1.
Crossref
Yasuyoshi Kimura, Masaaki Oda, Tsunetoshi Nakatani, Yoichi Sekita, Asun Monfort, Anton Wutz, Hideki Mochizuki & Toru Nakano. (2015) CRISPR/Cas9-mediated reporter knock-in in mouse haploid embryonic stem cells. Scientific Reports 5:1.
Crossref
Hongsuk Park, Sungpil Cho, Yong Hwan Han, Margit M. Janat-Amsbury, Sihem Boudina & You Han Bae. (2015) Combinatorial gene construct and non-viral delivery for anti-obesity in diet-induced obese mice. Journal of Controlled Release 207, pages 154-162.
Crossref
Brennan S. Dirk, Rajesh Abraham Jacob, Aaron L. Johnson, Emily N. Pawlak, P. Craig Cavanagh, Logan Van Nynatten, S. M. Mansour Haeryfar & Jimmy D. Dikeakos. (2015) Viral Bimolecular Fluorescence Complementation: A Novel Tool to Study Intracellular Vesicular Trafficking Pathways. PLOS ONE 10:4, pages e0125619.
Crossref
Christopher B. Chambers, William P. Halford, Joshua Geltz, Olga Villamizar, Jeffrey Gross, Alison Embalabala, Edward Gershburg & Andrew Wilber. (2015) A System for Creating Stable Cell Lines that Express a Gene of Interest from a Bidirectional and Regulatable Herpes Simplex Virus Type 1 Promoter. PLOS ONE 10:3, pages e0122253.
Crossref
Sayed Hoseini & Martin G Sauer. (2015) Molecular cloning using polymerase chain reaction, an educational guide for cellular engineering. Journal of Biological Engineering 9:1, pages 2.
Crossref
Hua Lu, Tianlun Jiang, Ruqing Li, Shichun Wang, Qiang Zhang & Shuming Zhao. (2015) Bone marrow stromal cells transduced with a thrombopoietin, interleukin-6, and interleukin-11 syncretic gene induce cord mononuclear cells to generate platelets in vitro. Transfusion 55:1, pages 176-186.
Crossref
Lucia Mazzacurati, Marco Marzulli, Bonnie Reinhart, Yoshitaka Miyagawa, Hiroaki Uchida, William F Goins, Aofei Li, Balveen Kaur, Michael Caligiuri, Timothy Cripe, Nino Chiocca, Nduka Amankulor, Justus B Cohen, Joseph C Glorioso & Paola Grandi. (2015) Use of miRNA Response Sequences to Block Off-target Replication and Increase the Safety of an Unattenuated, Glioblastoma-targeted Oncolytic HSV. Molecular Therapy 23:1, pages 99-107.
Crossref
Yide Huang, Ruonan Zhen, Meiqin Jiang, Jie Yang, Yun Yang, Zhen Huang & Yao Lin. (2015) Development of all-in-one multicistronic Tet-On lentiviral vectors for inducible co-expression of two transgenes. Biotechnology and Applied Biochemistry 62:1, pages 48-54.
Crossref
Danielle A. Fanslow, Stacey E. Wirt, Jenny C. Barker, Jon P. Connelly, Matthew H. Porteus & Christina Tenenhaus Dann. (2014) Genome Editing in Mouse Spermatogonial Stem/Progenitor Cells Using Engineered Nucleases. PLoS ONE 9:11, pages e112652.
Crossref
Wen Hao Neo, Jun Feng Lim, Raelene Grumont, Steve Gerondakis & I-hsin Su. (2014) c-Rel Regulates Ezh2 Expression in Activated Lymphocytes and Malignant Lymphoid Cells. Journal of Biological Chemistry 289:46, pages 31693-31707.
Crossref
Karolina Malecek, Arsen Grigoryan, Shi Zhong, Wei Jun Gu, Laura A. Johnson, Steven A. Rosenberg, Timothy Cardozo & Michelle Krogsgaard. (2014) Specific Increase in Potency via Structure-Based Design of a TCR. The Journal of Immunology 193:5, pages 2587-2599.
Crossref
Young-Hoon Kim, Suresh Ramakrishna, Hyongbum Kim & Jin-Soo Kim. (2014) Enrichment of cells with TALEN-induced mutations using surrogate reporters. Methods 69:1, pages 108-117.
Crossref
Anna Pertek, Florian Meier, Martin Irmler, Johannes Beckers, Stavroula Skylaki, Max Endele, Wolfgang Wurst, Nilima Prakash & Ralf Kühn. (2014) Simple Derivation of Transgene-Free iPS Cells by a Dual Recombinase Approach. Molecular Biotechnology 56:8, pages 697-713.
Crossref
Matthew L. Bettini, Clifford Guy, Pradyot Dash, Kate M. Vignali, David E. Hamm, Jessica Dobbins, Etienne Gagnon, Paul G. Thomas, Kai W. Wucherpfennig & Dario A. A. Vignali. (2014) Membrane Association of the CD3ε Signaling Domain Is Required for Optimal T Cell Development and Function. The Journal of Immunology 193:1, pages 258-267.
Crossref
Katarzyna Duda, Lindsey A. Lonowski, Michael Kofoed-Nielsen, Adriana Ibarra, Catherine M. Delay, Qiaohua Kang, Zhang Yang, Shondra M. Pruett-Miller, Eric P. Bennett, Hans H. Wandall, Gregory D. Davis, Steen H. Hansen & Morten Frödin. (2014) High-efficiency genome editing via 2A-coupled co-expression of fluorescent proteins and zinc finger nucleases or CRISPR/Cas9 nickase pairs. Nucleic Acids Research 42:10, pages e84-e84.
Crossref
L. Jung, P. Tropel, Y. Moal, M. Teletin, E. Jeandidier, R. Gayon, C. Himmelspach, F. Bello, C. Andre, A. Tosch, A. Mansouri, C. Bruant-Rodier, P. Bouille & S. Viville. (2014) ONSL and OSKM cocktails act synergistically in reprogramming human somatic cells into induced pluripotent stem cells. Molecular Human Reproduction 20:6, pages 538-549.
Crossref
Sol Ji Park, Bumrae Cho, Ok Jae Koo, Hwajung Kim, Jung Taek Kang, Sunghoon Hurh, Su Jin Kim, Hye Jung Yeom, Joonho Moon, Eun Mi Lee, Ji Yei Choi, Ju Ho Hong, Goo Jang, Joing-Ik Hwang, Jaeseok Yang, Byeong Chun Lee & Curie Ahn. (2014) Production and characterization of soluble human TNFRI-Fc and human HO-1(HMOX1) transgenic pigs by using the F2A peptide. Transgenic Research 23:3, pages 407-419.
Crossref
Spyridon Stavrou, Daniel Crawford, Kristin Blouch, Edward P. Browne, Rahul M. Kohli & Susan R. Ross. (2014) Different Modes of Retrovirus Restriction by Human APOBEC3A and APOBEC3G In Vivo. PLoS Pathogens 10:5, pages e1004145.
Crossref
Rachel A. Levin, Csilla N. Felsen, Jin Yang, John Y. Lin, Michael A. Whitney, Quyen T. Nguyen & Roger Y. Tsien. (2014) An Optimized Triple Modality Reporter for Quantitative In Vivo Tumor Imaging and Therapy Evaluation. PLoS ONE 9:5, pages e97415.
Crossref
Tingting Xu, Steven Ripp, Gary S. Sayler & Dan M. Close. (2014) Expression of a Humanized Viral 2A-Mediated lux Operon Efficiently Generates Autonomous Bioluminescence in Human Cells. PLoS ONE 9:5, pages e96347.
Crossref
Suveena Sharma & Jinfang Zhu. (2014) Immunologic Applications of Conditional Gene Modification Technology in the Mouse. Current Protocols in Immunology 105:1.
Crossref
Blaise Ndjamen, Alexander H. Farley, Terri Lee, Scott E. Fraser & Pamela J. Bjorkman. (2014) The Herpes Virus Fc Receptor gE-gI Mediates Antibody Bipolar Bridging to Clear Viral Antigens from the Cell Surface. PLoS Pathogens 10:3, pages e1003961.
Crossref
Arnaud Ahier & Sophie Jarriault. (2014) Simultaneous Expression of Multiple Proteins Under a Single Promoter in Caenorhabditis elegans via a Versatile 2A-Based Toolkit . Genetics 196:3, pages 605-613.
Crossref
Jennifer S. Chambers, Tomoyuki Tanaka, Tim Brend, Hanif Ali, Nicola J. Geisler, Leah Khazin, Juan C. Cigudosa, T. Neil Dear, Kenneth MacLennan & Terence H. Rabbitts. (2014) Sequential Gene Targeting to Make Chimeric Tumor Models with De Novo Chromosomal Abnormalities . Cancer Research 74:5, pages 1588-1597.
Crossref
N D Fagoe, R Eggers, J Verhaagen & M R J Mason. (2013) A compact dual promoter adeno-associated viral vector for efficient delivery of two genes to dorsal root ganglion neurons. Gene Therapy 21:3, pages 242-252.
Crossref
Philipp Mergenthaler, Kristin Wendland & Andreas Meisel. (2014) A versatile tool for the analysis of neuronal survival. Methods 66:2, pages 325-329.
Crossref
F Alaee, O Sugiyama, M S Virk, H Tang, H Drissi, A C Lichtler & J R Lieberman. (2013) Suicide gene approach using a dual-expression lentiviral vector to enhance the safety of ex vivo gene therapy for bone repair. Gene Therapy 21:2, pages 139-147.
Crossref
Sylvia Merkert, Stephanie Wunderlich, Christien Bednarski, Jennifer Beier, Alexandra Haase, Anne-Kathrin Dreyer, Kristin Schwanke, Johann Meyer, Gudrun Göhring, Toni Cathomen & Ulrich Martin. (2014) Efficient Designer Nuclease-Based Homologous Recombination Enables Direct PCR Screening for Footprintless Targeted Human Pluripotent Stem Cells. Stem Cell Reports 2:1, pages 107-118.
Crossref
Christos S. Karamitros & Manfred Konrad. (2014) Bacterial co-expression of the ? and ? protomers of human l-asparaginase-3: Achieving essential N-terminal exposure of a catalytically critical threonine located in the ?-subunit. Protein Expression and Purification 93, pages 1-10.
Crossref
Tingting Xu, Nicole Perry, Archana Chuahan, Gary Sayler & Steven Ripp. 2014. Microbial Biodegradation and Bioremediation. Microbial Biodegradation and Bioremediation 115 136 .
Elisa Kieback, Ellen Hilgenberg & Simon Fillatreau. 2014. T-Helper Cells. T-Helper Cells 117 126 .
Marioara F. Ciuculescu, Christian Brendel, Chad E. Harris & David A. Williams. 2014. Hematopoietic Stem Cell Protocols. Hematopoietic Stem Cell Protocols 287 309 .
June-Key Chung, Hyewon Youn, Joo Hyun Kang & Keon Wook Kang. 2014. Molecular Imaging of Small Animals. Molecular Imaging of Small Animals 685 713 .
Claudia Cavelti-Weder, Weida Li, Gordon C. Weir & Qiao Zhou. 2014. Stem Cell Transcriptional Networks. Stem Cell Transcriptional Networks 247 262 .
Hyewon YounJune-Key Chung. (2013) Reporter Gene Imaging. American Journal of Roentgenology 201:2, pages W206-W214.
Crossref
Alejandro FerrerAdam G. SchrumDiana Gil. (2013) A PCR-Based Method to Genotype Mice Knocked Out for All Four CD3 Subunits, the Standard Recipient Strain for Retrogenic TCR/CD3 Bone Marrow Reconstitution Technology. BioResearch Open Access 2:3, pages 222-226.
Crossref
Karolina Malecek, Shi Zhong, Katelyn McGary, Connie Yu, Kevin Huang, Laura A. Johnson, Steven A. Rosenberg & Michelle Krogsgaard. (2013) Engineering improved T cell receptors using an alanine-scan guided T cell display selection system. Journal of Immunological Methods 392:1-2, pages 1-11.
Crossref
Jun Yan, Hailin Yang, Guohua Yue & Wenda Gao. (2013) A mini-IRES sequence for stringent selection of high producers. Journal of Biosciences 38:2, pages 245-249.
Crossref
Branden S. Moriarity, Eric P. Rahrmann, Vincent W. Keng, Luke S. Manlove, Dominic A. Beckmann, Natalie K. Wolf, Touba Khurshid, Jason B. Bell & David A. Largaespada. (2013) Modular assembly of transposon integratable multigene vectors using RecWay assembly. Nucleic Acids Research 41:8, pages e92-e92.
Crossref
J-Y Shin, Y-S Chung, B Kang, H-L Jiang, D-Y Yu, K Han, C Chae, J-H Moon, G Jang & M-H Cho. (2013) Co-delivery of LETM1 and CTMP synergistically inhibits tumor growth in H-ras12V liver cancer model mice. Cancer Gene Therapy 20:3, pages 186-194.
Crossref
Yu Imuta, Hiroshi Kiyonari, Chuan‐Wei Jang, Richard R. Behringer & Hiroshi Sasaki. (2013) Generation of knock‐in mice that express nuclear enhanced green fluorescent protein and tamoxifen‐inducible Cre recombinase in the notochord from Foxa2 and T loci . genesis 51:3, pages 210-218.
Crossref
Giovane G. Tortelote, J. Manuel Hernández-Hernández, Alexandre J.C. Quaresma, Jeffrey A. Nickerson, Anthony N. Imbalzano & Jaime A. Rivera-Pérez. (2013) Wnt3 function in the epiblast is required for the maintenance but not the initiation of gastrulation in mice. Developmental Biology 374:1, pages 164-173.
Crossref
Claudia Merkl, Anja Saalfrank, Nathalie Riesen, Ralf Kühn, Anna Pertek, Stefan Eser, Markus Sebastian Hardt, Alexander Kind, Dieter Saur, Wolfgang Wurst, Antonio Iglesias & Angelika Schnieke. (2013) Efficient Generation of Rat Induced Pluripotent Stem Cells Using a Non-Viral Inducible Vector. PLoS ONE 8:1, pages e55170.
Crossref
Ekaterina Minskaia & Martin D. Ryan. (2013) Protein Coexpression Using FMDV 2A: Effect of ?Linker? Residues. BioMed Research International 2013, pages 1-12.
Crossref
Iacovos P Michael, Claudio Monetti, Anthony C Chiu, Puzheng Zhang, Takeshi Baba, Koichiro Nishino, Siamak Agha-Mohammadi, Knut Woltjen, Hoon-Ki Sung & Andras Nagy. (2012) Highly efficient site-specific transgenesis in cancer cell lines. Molecular Cancer 11:1.
Crossref
Stephanie WunderlichAlexandra HaaseSylvia MerkertJennifer BeierKristin SchwankeAxel SchambachSilke GlageGudrun GöhringEliza C. CurnowUlrich Martin. (2012) Induction of Pluripotent Stem Cells from a Cynomolgus Monkey Using a Polycistronic Simian Immunodeficiency Virus–Based Vector, Differentiation Toward Functional Cardiomyocytes, and Generation of Stably Expressing Reporter Lines. Cellular Reprogramming 14:6, pages 471-484.
Crossref
Kenneth K. Yu, Kiefer Aguilar, Jonathan Tsai, Rachel Galimidi, Priyanthi Gnanapragasam, Lili Yang & David Baltimore. (2012) Use of Mutated Self-Cleaving 2A Peptides as a Molecular Rheostat to Direct Simultaneous Formation of Membrane and Secreted Anti-HIV Immunoglobulins. PLoS ONE 7:11, pages e50438.
Crossref
P Maier, D Heckmann, I Spier, S Laufs, M Zucknick, H Allgayer, S Fruehauf, W J Zeller & F Wenz. (2012) F2A sequence linking MGMTP140K and MDR1 in a bicistronic lentiviral vector enables efficient chemoprotection of haematopoietic stem cells. Cancer Gene Therapy 19:11, pages 802-810.
Crossref
Michael T Certo, Kamila S Gwiazda, Ryan Kuhar, Blythe Sather, Gabrielle Curinga, Tyler Mandt, Michelle Brault, Abigail R Lambert, Sarah K Baxter, Kyle Jacoby, Byoung Y Ryu, Hans-Peter Kiem, Agnes Gouble, Frederic Paques, David J Rawlings & Andrew M Scharenberg. (2012) Coupling endonucleases with DNA end–processing enzymes to drive gene disruption. Nature Methods 9:10, pages 973-975.
Crossref
Stephen A. Murray, Janan T. Eppig, Damian Smedley, Elizabeth M. Simpson & Nadia Rosenthal. (2012) Beyond knockouts: cre resources for conditional mutagenesis. Mammalian Genome 23:9-10, pages 587-599.
Crossref
Anders Kielland, Laura M. A. Camassa, Gaute Døhlen, Ludvig A. Munthe, Rune Blomhoff, Mahmood Amiry-Moghaddam & Harald Carlsen. (2012) NF-κB Activity in Perinatal Brain During Infectious and Hypoxic-Ischemic Insults Revealed by a Reporter Mouse. Brain Pathology 22:4, pages 499-510.
Crossref
Matthew L. Bettini, Maria Bettini & Dario A. A. Vignali. (2012) T‐cell receptor retrogenic mice: a rapid, flexible alternative to T‐cell receptor transgenic mice. Immunology 136:3, pages 265-272.
Crossref
Anusara Daenthanasanmak, Gustavo Salguero, Sylvia Borchers, Constanca Figueiredo, Roland Jacobs, Bala Sai Sundarasetty, Andreas Schneider, Axel Schambach, Britta Eiz-Vesper, Rainer Blasczyk, Eva M. Weissinger, Arnold Ganser & Renata Stripecke. (2012) Integrase-defective lentiviral vectors encoding cytokines induce differentiation of human dendritic cells and stimulate multivalent immune responses in vitro and in vivo. Vaccine 30:34, pages 5118-5131.
Crossref
M. Bettini, A. H. Castellaw, G. P. Lennon, A. R. Burton & D. A. A. Vignali. (2012) Prevention of Autoimmune Diabetes by Ectopic Pancreatic  -Cell Expression of Interleukin-35. Diabetes 61:6, pages 1519-1526.
Crossref
Andrea L. Szymczak-Workman, Kate M. Vignali & Dario A.A. Vignali. (2012) Design and Construction of 2A Peptide-Linked Multicistronic Vectors. Cold Spring Harbor Protocols 2012:2, pages pdb.ip067876.
Crossref
Mudita PinchaBala Sai SundarasettyGustavo SalgueroRalf GutzmerHenk GarritsenLaura MackeAndreas SchneiderDaniela LenzConstanca FigueiredoRainer BlasczykEliana RuggieroManfred SchmidtChristof von KalleChristina PuffUte ModlichHeiko von der LeyenDaniel C. WickeArnold GanserRenata Stripecke. (2012) Identity, Potency, In Vivo Viability, and Scaling Up Production of Lentiviral Vector-Induced Dendritic Cells for Melanoma Immunotherapy . Human Gene Therapy Methods 23:1, pages 38-55.
Crossref
Efrat Merhavi-Shoham, Astar Haga-Friedman & Cyrille J. Cohen. (2012) Genetically modulating T-cell function to target cancer. Seminars in Cancer Biology 22:1, pages 14-22.
Crossref
Hidehiko K. Inagaki, Shlomo Ben-Tabou de-Leon, Allan M. Wong, Smitha Jagadish, Hiroshi Ishimoto, Gilad Barnea, Toshihiro Kitamoto, Richard Axel & David J. Anderson. (2012) Visualizing Neuromodulation In Vivo: TANGO-Mapping of Dopamine Signaling Reveals Appetite Control of Sugar Sensing. Cell 148:3, pages 583-595.
Crossref
Tristen S. Park, Steven A. Rosenberg & Richard A. Morgan. (2011) Treating cancer with genetically engineered T cells. Trends in Biotechnology 29:11, pages 550-557.
Crossref
Gustavo SalgueroBala Sai SundarasettySylvia BorchersDirk WedekindBritta Eiz-VesperSarvari VelagaAdan C. JirmoGeorg BehrensGregor WarneckeAnn-Kathrin KnöfelRainer BlasczykEva Mischak-WeissingerArnold GanserRenata Stripecke. (2011) Preconditioning Therapy with Lentiviral Vector-Programmed Dendritic Cells Accelerates the Homeostatic Expansion of Antigen-Reactive Human T Cells in NOD.Rag1 −/− .IL-2rγc −/− mice . Human Gene Therapy 22:10, pages 1209-1224.
Crossref
Pimprapar Wongsrikeao, Dyana Saenz, Tommy Rinkoski, Takeshige Otoi & Eric Poeschla. (2011) Antiviral restriction factor transgenesis in the domestic cat. Nature Methods 8:10, pages 853-859.
Crossref
Eugene V. Barsov, Matthew T. Trivett, Jacob T. Minang, Haosi Sun, Claes Ohlen & David E. Ott. (2011) Transduction of SIV-Specific TCR Genes into Rhesus Macaque CD8+ T Cells Conveys the Ability to Suppress SIV Replication. PLoS ONE 6:8, pages e23703.
Crossref
M Pincha, G Salguero, D Wedekind, B S Sundarasetty, A Lin, N Kasahara, M H Brugman, A C Jirmo, U Modlich, R Gutzmer, G Büsche, A Ganser & R Stripecke. (2011) Lentiviral vectors for induction of self-differentiation and conditional ablation of dendritic cells. Gene Therapy 18:8, pages 750-764.
Crossref
Guangming Wu, Na Liu, Ina Rittelmeyer, Amar Deep Sharma, Malte Sgodda, Holm Zaehres, Martina Bleidi?el, Boris Greber, Luca Gentile, Dong Wook Han, Cornelia Rudolph, Doris Steinemann, Axel Schambach, Michael Ott, Hans R. Sch?ler & Tobias Cantz. (2011) Generation of Healthy Mice from Gene-Corrected Disease-Specific Induced Pluripotent Stem Cells. PLoS Biology 9:7, pages e1001099.
Crossref
Caroline Vandeputte, Nele Evens, Jaan Toelen, Christophe M. Deroose, Barbara Bosier, Abdelilah Ibrahimi, Anke Van der Perren, Rik Gijsbers, Peter Janssen, Didier M. Lambert, Alfons Verbruggen, Zeger Debyser, Guy Bormans, Veerle Baekelandt & Koen Van Laere. (2011) A PET Brain Reporter Gene System Based on Type 2 Cannabinoid Receptors. Journal of Nuclear Medicine 52:7, pages 1102-1109.
Crossref
Harold A. Chapman, Xiaopeng Li, Jonathan P. Alexander, Alexis Brumwell, Walter Lorizio, Kevin Tan, Arnoud Sonnenberg, Ying Wei & Thiennu H. Vu. (2011) Integrin α6β4 identifies an adult distal lung epithelial population with regenerative potential in mice. Journal of Clinical Investigation 121:7, pages 2855-2862.
Crossref
Go Shioi, Hiroshi Kiyonari, Takaya Abe, Kazuki Nakao, Toshihiko Fujimori, Chuan-Wei Jang, Cheng-Chiu Huang, Haruhiko Akiyama, Richard R. Behringer & Shinichi Aizawa. (2011) A mouse reporter line to conditionally mark nuclei and cell membranes for in vivo live-imaging. genesis 49:7, pages 570-578.
Crossref
Anja Marquardt, Stephan Halle, Christof K. Seckert, Niels A. W. Lemmermann, Tibor Z. Veres, Armin Braun, Ulrich A. Maus, Reinhold Förster, Matthias J. Reddehase, Martin Messerle & Andreas Busche. (2011) Single cell detection of latent cytomegalovirus reactivation in host tissue. Journal of General Virology 92:6, pages 1279-1291.
Crossref
Jin Hee Kim, Sang-Rok Lee, Li-Hua Li, Hye-Jeong Park, Jeong-Hoh Park, Kwang Youl Lee, Myeong-Kyu Kim, Boo Ahn Shin & Seok-Yong Choi. (2011) High Cleavage Efficiency of a 2A Peptide Derived from Porcine Teschovirus-1 in Human Cell Lines, Zebrafish and Mice. PLoS ONE 6:4, pages e18556.
Crossref
Eva Warlich, Johannes Kuehle, Tobias Cantz, Martijn H Brugman, Tobias Maetzig, Melanie Galla, Adam A Filipczyk, Stephan Halle, Hannes Klump, Hans R Sch?ler, Christopher Baum, Timm Schroeder & Axel Schambach. (2011) Lentiviral Vector Design and Imaging Approaches to Visualize the Early Stages of Cellular Reprogramming. Molecular Therapy 19:4, pages 782-789.
Crossref
Helmut Hofemeister, Giovanni Ciotta, Jun Fu, Philipp Martin Seibert, Alexander Schulz, Marcello Maresca, Mihail Sarov, Konstantinos Anastassiadis & A. Francis Stewart. (2011) Recombineering, transfection, Western, IP and ChIP methods for protein tagging via gene targeting or BAC transgenesis. Methods 53:4, pages 437-452.
Crossref
Patrick Maier, Christof von Kalle & Stephanie Laufs. (2010) Retroviral vectors for gene therapy. Future Microbiology 5:10, pages 1507-1523.
Crossref
Edwin P. Alyea, Daniel J. DeAngelo, Jeffrey Moldrem, John M. Pagel, Donna Przepiorka, Michel Sadelin, James W. Young, Sergio Giralt, Michael Bishop & Stan Riddell. (2010) NCI First International Workshop on the Biology, Prevention and Treatment of Relapse after Allogeneic Hematopoietic Cell Transplantation: Report from the Committee on Prevention of Relapse Following Allogeneic Cell Transplantation for Hematologic Malignancies. Biology of Blood and Marrow Transplantation 16:8, pages 1037-1069.
Crossref
Todd M. Brusko, Richard C. Koya, Shirley Zhu, Michael R. Lee, Amy L. Putnam, Stephanie A. McClymont, Michael I. Nishimura, Shuhong Han, Lung-Ji Chang, Mark A. Atkinson, Antoni Ribas & Jeffrey A. Bluestone. (2010) Human Antigen-Specific Regulatory T Cells Generated by T Cell Receptor Gene Transfer. PLoS ONE 5:7, pages e11726.
Crossref
Michael Dalgaard Mikkelsen, Carl Erik Olsen & Barbara Ann Halkier. (2010) Production of the Cancer-Preventive Glucoraphanin in Tobacco. Molecular Plant 3:4, pages 751-759.
Crossref
Amanda R. Burton, Zachary Baquet, George S. Eisenbarth, Roland Tisch, Richard Smeyne, Creg J. Workman & Dario A. A. Vignali. (2010) Central Nervous System Destruction Mediated by Glutamic Acid Decarboxylase-Specific CD4+ T Cells. The Journal of Immunology 184:9, pages 4863-4870.
Crossref
John C. Markley & Michel Sadelain. (2010) IL-7 and IL-21 are superior to IL-2 and IL-15 in promoting human T cell–mediated rejection of systemic lymphoma in immunodeficient mice. Blood 115:17, pages 3508-3519.
Crossref
Jun Yan, Hongjun Wang, Qian Xu, Nidhi Jain, Vasilis Toxavidis, John Tigges, Hailin Yang, Guohua Yue & Wenda Gao. (2010) Signal sequence is still required in genes downstream of ?autocleaving? 2A peptide for secretary or membrane-anchored expression. Analytical Biochemistry 399:1, pages 144-146.
Crossref
Zhilong Yang & Bernard Moss. (2009) Interaction of the Vaccinia Virus RNA Polymerase-Associated 94-Kilodalton Protein with the Early Transcription Factor. Journal of Virology 83:23, pages 12018-12026.
Crossref
Michel Sadelain. (2009) T-Cell Engineering for Cancer Immunotherapy. The Cancer Journal 15:6, pages 451-455.
Crossref
Kanako Ukita, Shino Hirahara, Naoko Oshima, Yu Imuta, Aki Yoshimoto, Chuan-Wei Jang, Masayuki Oginuma, Yumiko Saga, Richard R. Behringer, Hisato Kondoh & Hiroshi Sasaki. (2009) Wnt signaling maintains the notochord fate for progenitor cells and supports the posterior extension of the notochord. Mechanisms of Development 126:10, pages 791-803.
Crossref
M. David Stewart, Chuan‐Wei Jang, Nicholas W. Hong, Anthony P. Austin & Richard R. Behringer. (2009) Dual fluorescent protein reporters for studying cell behaviors in vivo. genesis 47:10, pages 708-717.
Crossref
Steven Froelich, Leslie Ziegler, Katie Stroup & Pin Wang. (2009) Targeted gene delivery to CD117-expressing cells in vivo with lentiviral vectors co-displaying stem cell factor and a fusogenic molecule. Biotechnology and Bioengineering 104:1, pages 206-215.
Crossref
Abdelilah IbrahimiGreetje Vande VeldeVeerle ReumersJaan ToelenIrina ThiryCaroline VandeputteSofie VetsChristophe DerooseGuy BormansVeerle BaekelandtZeger DebyserRik Gijsbers. (2009) Highly Efficient Multicistronic Lentiviral Vectors with Peptide 2A Sequences. Human Gene Therapy 20:8, pages 845-860.
Crossref
Sonja Nowotschin, Guy S. Eakin & Anna‐Katerina Hadjantonakis. (2009) Dual transgene strategy for live visualization of chromatin and plasma membrane dynamics in murine embryonic stem cells and embryonic tissues. genesis 47:5, pages 330-336.
Crossref
Marco J. HeroldJens van den Brandt, Jost Seibler & Holger M. Reichardt. (2008) Inducible and reversible gene silencing by stable integration of an shRNA-encoding lentivirus in transgenic rats. Proceedings of the National Academy of Sciences 105:47, pages 18507-18512.
Crossref
S Yang, C J Cohen, P D Peng, Y Zhao, L Cassard, Z Yu, Z Zheng, S Jones, N P Restifo, S A Rosenberg & R A Morgan. (2008) Development of optimal bicistronic lentiviral vectors facilitates high-level TCR gene expression and robust tumor cell recognition. Gene Therapy 15:21, pages 1411-1423.
Crossref
Nancy Smyth TempletonAxel Schambach, Tobias Maetzig & Christopher Baum. 2008. Gene and Cell Therapy. Gene and Cell Therapy.
Haiguang Yang, Leslie Ziegler, Kye‐Il Joo, Taehoon Cho, Yuning Lei & Pin Wang. (2008) Gamma‐retroviral vectors enveloped with an antibody and an engineered fusogenic protein achieved antigen‐specific targeting. Biotechnology and Bioengineering 101:2, pages 357-368.
Crossref
Jeff Holst, Haopeng Wang, Kelly Durick Eder, Creg J Workman, Kelli L Boyd, Zachary Baquet, Harvir Singh, Karen Forbes, Andrzej Chruscinski, Richard Smeyne, Nicolai S C van Oers, Paul J Utz & Dario A A Vignali. (2008) Scalable signaling mediated by T cell antigen receptor–CD3 ITAMs ensures effective negative selection and prevents autoimmunity. Nature Immunology 9:6, pages 658-666.
Crossref
A. R. Burton, E. Vincent, P. Y. Arnold, G. P. Lennon, M. Smeltzer, C.-S. Li, K. Haskins, J. Hutton, R. M. Tisch, E. E. Sercarz, P. Santamaria, C. J. Workman & D. A.A. Vignali. (2008) On the Pathogenicity of Autoantigen-Specific T-Cell Receptors. Diabetes 57:5, pages 1321-1330.
Crossref
Lauren W. Collison, Creg J. Workman, Timothy T. Kuo, Kelli Boyd, Yao Wang, Kate M. Vignali, Richard Cross, David Sehy, Richard S. Blumberg & Dario A. A. Vignali. (2007) The inhibitory cytokine IL-35 contributes to regulatory T-cell function. Nature 450:7169, pages 566-569.
Crossref
Minghong Jiang, Wenfang Shi, Qi Zhang, Xinhua Wang, Minggao Guo, Zhenfu Cui, Changqin Su, Qing Yang, Yuemin Li, Jonathan Sham, Xinyuan Liu, Mengchao Wu & Qijun Qian. (2006) Gene Therapy Using Adenovirus-Mediated Full-length Anti-HER-2 Antibody for HER-2 Overexpression Cancers. Clinical Cancer Research 12:20, pages 6179-6185.
Crossref
Jeff Holst, Andrea L Szymczak-Workman, Kate M Vignali, Amanda R Burton, Creg J Workman & Dario A A Vignali. (2006) Generation of T-cell receptor retrogenic mice. Nature Protocols 1:1, pages 406-417.
Crossref
Pablo de Felipe, Garry A. Luke, Lorraine E. Hughes, David Gani, Claire Halpin & Martin D. Ryan. (2006) E unum pluribus: multiple proteins from a self-processing polyprotein. Trends in Biotechnology 24:2, pages 68-75.
Crossref

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.