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Translational value of liquid chromatography coupled with tandem mass spectrometry-based quantitative proteomics for in vitroin vivo extrapolation of drug metabolism and transport and considerations in selecting appropriate techniques

, BSc MSc (PhD Candidate) , , MPharm PhD (Research Associate) , , PharmD PhD (Professor of Systems Pharmacology) & , MA PhD (Reader in Pharmacy)

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Yasmine Elmorsi, Hajar Al Feteisi, Zubida M. Al-Majdoub, Jill Barber, Amin Rostami-Hodjegan & Brahim Achour. (2020) Proteomic characterisation of drug metabolising enzymes and drug transporters in pig liver. Xenobiotica 50:10, pages 1208-1219.
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Martyn Howard, Jill Barber, Naved Alizai & Amin Rostami-Hodjegan. (2018) Dose adjustment in orphan disease populations: the quest to fulfill the requirements of physiologically based pharmacokinetics. Expert Opinion on Drug Metabolism & Toxicology 14:12, pages 1315-1330.
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Articles from other publishers (22)

Areti-Maria Vasilogianni, Sarah Alrubia, Eman El-Khateeb, Zubida M. Al-Majdoub, Narciso Couto, Brahim Achour, Amin Rostami-Hodjegan & Jill Barber. (2024) Complementarity of two proteomic data analysis tools in the identification of drug-metabolising enzymes and transporters in human liver. Molecular Omics.
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Xuan Guo, Lei Zhang, Zihan Lei, Zhe Hou, Hui Li, Xiaodong Li, Jing Dong, Ling Song, Dingding Chen & Dongyang Liu. (2023) A simple LC-MS/MS method for the simultaneous quantification of drug metabolic enzymes. Journal of Chromatography B 1214, pages 123536.
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Deepak Ahire, Laken Kruger, Sheena Sharma, Vijaya Saradhi Mettu, Abdul Basit & Bhagwat Prasad. (2022) Quantitative Proteomics in Translational Absorption, Distribution, Metabolism, and Excretion and Precision Medicine. Pharmacological Reviews 74:3, pages 771-798.
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Yurong Lai, Xiaoyan Chu, Li Di, Wei Gao, Yingying Guo, Xingrong Liu, Chuang Lu, Jialin Mao, Hong Shen, Huaping Tang, Cindy Q. Xia, Lei Zhang & Xinxin Ding. (2022) Recent advances in the translation of drug metabolism and pharmacokinetics science for drug discovery and development. Acta Pharmaceutica Sinica B 12:6, pages 2751-2777.
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Deepak Suresh Ahire, Abdul Basit, Matthew Karasu & Bhagwat Prasad. (2021) Ultrasensitive Quantification of Drug-metabolizing Enzymes and Transporters in Small Sample Volume by Microflow LC-MS/MS. Journal of Pharmaceutical Sciences 110:7, pages 2833-2840.
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Jiapeng Li & Hao-Jie Zhu. (2020) Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)-Based Proteomics of Drug-Metabolizing Enzymes and Transporters. Molecules 25:11, pages 2718.
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Eman El-Khateeb, Areti-Maria Vasilogianni, Sarah Alrubia, Zubida M. Al-Majdoub, Narciso Couto, Martyn Howard, Jill Barber, Amin Rostami-Hodjegan & Brahim Achour. (2019) Quantitative mass spectrometry-based proteomics in the era of model-informed drug development: Applications in translational pharmacology and recommendations for best practice. Pharmacology & Therapeutics 203, pages 107397.
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Nicola Melillo, Adam S. Darwich, Paolo Magni & Amin Rostami-Hodjegan. (2019) Accounting for inter-correlation between enzyme abundance: a simulation study to assess implications on global sensitivity analysis within physiologically-based pharmacokinetics. Journal of Pharmacokinetics and Pharmacodynamics 46:2, pages 137-154.
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Hajar Al Feteisi, Zubida M. Al-Majdoub, Brahim Achour, Narciso Couto, Amin Rostami-Hodjegan & Jill Barber. (2018) Identification and quantification of blood-brain barrier transporters in isolated rat brain microvessels. Journal of Neurochemistry 146:6, pages 670-685.
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Brahim Achour, Alyssa Dantonio, Mark Niosi, Jonathan J. Novak, Zubida M. Al-Majdoub, Theunis C. Goosen, Amin Rostami-Hodjegan & Jill Barber. (2018) Data Generated by Quantitative Liquid Chromatography-Mass Spectrometry Proteomics Are Only the Start and Not the Endpoint: Optimization of Quantitative Concatemer-Based Measurement of Hepatic Uridine-5′-Diphosphate–Glucuronosyltransferase Enzymes with Reference to Catalytic Activity. Drug Metabolism and Disposition 46:6, pages 805-812.
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Qingcheng Mao, Yurong Lai & Joanne Wang. (2018) Drug Transporters in Xenobiotic Disposition and Pharmacokinetic Prediction. Drug Metabolism and Disposition 46:5, pages 561-566.
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Kosuke Doki, Adam S. Darwich, Brahim Achour, Aleksi Tornio, Janne T. Backman & Amin Rostami‐Hodjegan. (2018) Implications of intercorrelation between hepatic CYP3A4‐CYP2C8 enzymes for the evaluation of drug–drug interactions: a case study with repaglinide. British Journal of Clinical Pharmacology 84:5, pages 972-986.
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Julia Dittrich, Melanie Adam, Hilke Maas, Max Hecht, Madlen Reinicke, L. Renee Ruhaak, Christa Cobbaert, Christoph Engel, Kerstin Wirkner, Markus Löffler, Joachim Thiery & Uta Ceglarek. (2018) Targeted On-line SPE-LC-MS/MS Assay for the Quantitation of 12 Apolipoproteins from Human Blood. PROTEOMICS 18:3-4, pages 1700279.
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Amin Rostami-Hodjegan. (2018) Reverse Translation in PBPK and QSP: Going Backwards in Order to Go Forward With Confidence. Clinical Pharmacology & Therapeutics 103:2, pages 224-232.
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Brahim Achour, Alyssa Dantonio, Mark Niosi, Jonathan J. Novak, John K. Fallon, Jill Barber, Philip C. Smith, Amin Rostami-Hodjegan & Theunis C. Goosen. (2017) Quantitative Characterization of Major Hepatic UDP-Glucuronosyltransferase Enzymes in Human Liver Microsomes: Comparison of Two Proteomic Methods and Correlation with Catalytic Activity. Drug Metabolism and Disposition 45:10, pages 1102-1112.
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M Vrana, D Whittington, V Nautiyal & B Prasad. (2017) Database of Optimized Proteomic Quantitative Methods for Human Drug Disposition-Related Proteins for Applications in Physiologically Based Pharmacokinetic Modeling. CPT: Pharmacometrics & Systems Pharmacology 6:4, pages 267-276.
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Bhagwat Prasad, Marc Vrana, Aanchal Mehrotra, Katherine Johnson & Deepak Kumar Bhatt. (2017) The Promises of Quantitative Proteomics in Precision Medicine. Journal of Pharmaceutical Sciences 106:3, pages 738-744.
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Daniel Scotcher, Christopher Jones, Maria Posada, Aleksandra Galetin & Amin Rostami-Hodjegan. (2016) Key to Opening Kidney for In Vitro-In Vivo Extrapolation Entrance in Health and Disease: Part II: Mechanistic Models and In Vitro-In Vivo Extrapolation. The AAPS Journal 18:5, pages 1082-1094.
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Daniel Scotcher, Christopher Jones, Maria Posada, Amin Rostami-Hodjegan & Aleksandra Galetin. (2016) Key to Opening Kidney for In Vitro–In Vivo Extrapolation Entrance in Health and Disease: Part I: In Vitro Systems and Physiological Data. The AAPS Journal 18:5, pages 1067-1081.
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Geoffrey Tucker, Binodh DeSilva, Jennifer Dressman, Michiho Ito, Takuya Kumamoto, Don Mager, Hanns-Christian Mahler, Anke H. Maitland-van der Zee, Giovanni M. Pauletti, Hitoshi Sasaki, Vinod Shah, Daniel Tang & Michael Ward. (2016) Current Challenges and Potential Opportunities for the Pharmaceutical Sciences to Make Global Impact: An FIP Perspective. Journal of Pharmaceutical Sciences 105:9, pages 2489-2497.
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Y. Elmorsi, J. Barber & A. Rostami-Hodjegan. (2015) Ontogeny of Hepatic Drug Transporters and Relevance to Drugs Used in Pediatrics. Drug Metabolism and Disposition 44:7, pages 992-998.
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Brahim Achour, Zubida M. Al-Majdoub, Hajar Al Feteisi, Yasmine Elmorsi, Amin Rostami-Hodjegan & Jill Barber. (2015) Ten years of QconCATs: Application of multiplexed quantification to small medically relevant proteomes. International Journal of Mass Spectrometry 391, pages 93-104.
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