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The development of label-free cellular assays for drug discovery

Pages 1285-1298 | Published online: 01 Dec 2011

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Ye Fang. (2016) Compound annotation with real time cellular activity profiles to improve drug discovery. Expert Opinion on Drug Discovery 11:3, pages 269-280.
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Ye Fang, Jarrod French, Hong Zhao & Stephen Benkovic. (2013) G-protein-coupled receptor regulation of de novo purine biosynthesis: a novel druggable mechanism. Biotechnology and Genetic Engineering Reviews 29:1, pages 31-48.
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Ye Fang. (2012) Ligand–receptor interaction platforms and their applications for drug discovery. Expert Opinion on Drug Discovery 7:10, pages 969-988.
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Linjie Chen, Lili Jin & Naiming Zhou. (2012) An update of novel screening methods for GPCR in drug discovery. Expert Opinion on Drug Discovery 7:9, pages 791-806.
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Articles from other publishers (46)

Seyedyousef Arman, Richard D. Tilley & J. Justin Gooding. (2024) A review of electrochemical impedance as a tool for examining cell biology and subcellular mechanisms: merits, limits, and future prospects. The Analyst.
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Seyedyousef Arman, Vinicius R. Gonçales, Ying Yang, Richard D. Tilley, Katharina Gaus & J. Justin Gooding. (2023) Toward development of dual optical and electrical cell‐based biosensor: An investigation on electrode geometry and transparent conductive material function. Electroanalysis 35:11.
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María Jesús Pioz, Rocío L. Espinosa, María Fe Laguna, Beatriz Santamaria, Ana María M. Murillo, Álvaro Lavín Hueros, Sergio Quintero, Luca Tramarin, Luis G Valle, Pedro Herreros, Alberto Bellido, Rafael Casquel & Miguel Holgado. (2022) A review of Optical Point-of-Care devices to Estimate the Technology Transfer of These Cutting-Edge Technologies. Biosensors 12:12, pages 1091.
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Anna Vlachodimou, Henk de Vries, Milena Pasoli, Miranda Goudswaard, Soon-Ai Kim, Yong-Chul Kim, Mirko Scortichini, Melissa Marshall, Joel Linden, Laura H. Heitman, Kenneth A. Jacobson & Adriaan P. IJzerman. (2022) Kinetic profiling and functional characterization of 8-phenylxanthine derivatives as A2B adenosine receptor antagonists. Biochemical Pharmacology 200, pages 115027.
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Ulla Seibel-Ehlert, Nicole Plank, Asuka Inoue, Guenther Bernhardt & Andrea Strasser. (2021) Label-Free Investigations on the G Protein Dependent Signaling Pathways of Histamine Receptors. International Journal of Molecular Sciences 22:18, pages 9739.
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Shih-En Chou, Kuang-Li Lee, Pei-Kuen Wei & Ji-Yen Cheng. (2021) Screening anti-metastasis drugs by cell adhesion-induced color change in a biochip. Lab on a Chip 21:15, pages 2955-2970.
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Kuang-Li Lee, Hsien-San Hou, Ji-Yen Cheng & Pei-Kuen Wei. (2020) High-Throughput and Dynamic Study of Drug and Cell Interactions Using Contrast Images in Aluminum-Based Nanoslit Arrays. Analytical Chemistry 92:14, pages 9674-9681.
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Rong Wang, Yanfang Liu, Han Zhou, Yue Chen, Jixia Wang, Xiuli Zhang, Ruitao Yu & Xinmiao Liang. (2020) Integration of micro-fractionation, high-performance liquid chromatography-ultraviolet detector-charged aerosol detector-mass spectrometry analysis and cellular dynamic mass redistribution assay to accelerate alkaloid drug discovery. Journal of Chromatography A 1616, pages 460779.
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Anna Vlachodimou, Konstantina Konstantinopoulou, Adriaan P. IJzerman & Laura H. Heitman. (2020) Affinity, binding kinetics and functional characterization of draflazine analogues for human equilibrative nucleoside transporter 1 (SLC29A1). Biochemical Pharmacology 172, pages 113747.
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Han Zhou, Xingrong Peng, Tao Hou, Nan Zhao, Minghua Qiu, Xiuli Zhang & Xinmiao Liang. (2020) Identification of novel phytocannabinoids from Ganoderma by label-free dynamic mass redistribution assay. Journal of Ethnopharmacology 246, pages 112218.
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Anna Vlachodimou, Adriaan P. IJzerman & Laura H. Heitman. (2019) Label-free detection of transporter activity via GPCR signalling in living cells: A case for SLC29A1, the equilibrative nucleoside transporter 1. Scientific Reports 9:1.
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Weijia Zhou, Jixia Wang, Yaopeng Zhao, Long Yu, Ye Fang, Hongli Jin, Han Zhou, Pengyu Zhang, Yanfang Liu, Xiuli Zhang & Xinmiao Liang. (2018) Discovery of β2- adrenoceptor agonists in Curcuma zedoaria Rosc using label-free cell phenotypic assay combined with two-dimensional liquid chromatography. Journal of Chromatography A 1577, pages 59-65.
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Y. Fang. 2019. Label-Free Monitoring of Cells in vitro. Label-Free Monitoring of Cells in vitro 189 217 .
Y. Fang. 2017. Comprehensive Medicinal Chemistry III. Comprehensive Medicinal Chemistry III 416 433 .
I. Nederpelt, R.D. Vergroesen, A.P. IJzerman & L.H. Heitman. (2016) Persistent GnRH receptor activation in pituitary αT3-1 cells analyzed with a label-free technology. Biosensors and Bioelectronics 79, pages 721-727.
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Anders B. Klein, Mia Nittegaard-Nielsen, Julie T. Christensen, Anas Al-Khawaja & Petrine Wellendorph. (2016) Demonstration of the dynamic mass redistribution label-free technology as a useful cell-based pharmacological assay for endogenously expressed GABA A receptors . MedChemComm 7:3, pages 426-432.
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Mark Anderson, Emily O'Brien, Emily Grayek, James Hermansen & Heather Hunt. (2015) The Detection of Helicobacter hepaticus Using Whispering-Gallery Mode Microcavity Optical Sensors. Biosensors 5:3, pages 562-576.
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Fanyongjing Wang, Mark Anderson, Matthew Bernards & Heather Hunt. (2015) PEG Functionalization of Whispering Gallery Mode Optical Microresonator Biosensors to Minimize Non-Specific Adsorption during Targeted, Label-Free Sensing. Sensors 15:8, pages 18040-18060.
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Ye Fang. (2015) Total Internal Reflection Fluorescence Quantification of Receptor Pharmacology. Biosensors 5:2, pages 223-240.
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Jixia Wang, Tao Hou, Lai Wei, Liying Shi, Jian He, Nan Zhou, Guangwei Sun, Xiuli Zhang & Xinmiao Liang. (2015) Discovery of new targets of phenolic acids in danshen using a label-free cell phenotypic assay. RSC Advances 5:33, pages 25768-25776.
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Victor Yashunsky & Benjamin Aroeti. 2015. Label-Free Biosensor Methods in Drug Discovery. Label-Free Biosensor Methods in Drug Discovery 353 371 .
Ye Fang. 2015. Label-Free Biosensor Methods in Drug Discovery. Label-Free Biosensor Methods in Drug Discovery 17 33 .
Xinmiao Liang, Jixia Wang, Xiuli Zhang & Ye Fang. 2015. Label-Free Biosensor Methods in Drug Discovery. Label-Free Biosensor Methods in Drug Discovery 233 252 .
Haiyan Sun, Ying Wei, Huayun Deng, Qiaojie Xiong, Min Li, Joydeep Lahiri & Ye Fang. (2014) Label-free cell phenotypic profiling decodes the composition and signaling of an endogenous ATP-sensitive potassium channel. Scientific Reports 4:1.
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Ann M. Ferrie, Qi Wu, Oberon D. Deichmann & Ye Fang. (2014) High frequency resonant waveguide grating imager for assessing drug-induced cardiotoxicity. Applied Physics Letters 104:18.
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Ye Fang. (2014) Label-free drug discovery. Frontiers in Pharmacology 5.
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Norbert Orgovan, Boglarka Kovacs, Eniko Farkas, Bálint Szabó, Natalya Zaytseva, Ye Fang & Robert Horvath. (2014) Bulk and surface sensitivity of a resonant waveguide grating imager. Applied Physics Letters 104:8, pages 083506.
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Lenea Nørskov-Lauritsen, Alex Thomsen & Hans Bräuner-Osborne. (2014) G Protein-Coupled Receptor Signaling Analysis Using Homogenous Time-Resolved Förster Resonance Energy Transfer (HTRF®) Technology. International Journal of Molecular Sciences 15:2, pages 2554-2572.
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Ann M. Ferrie, Haiyan Sun, Natalya Zaytseva & Ye Fang. (2014) Divergent Label-free Cell Phenotypic Pharmacology of Ligands at the Overexpressed β2-Adrenergic Receptors. Scientific Reports 4:1.
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Bahareh Honarparvar, Thavendran Govender, Glenn E. M. Maguire, Mahmoud E. S. Soliman & Hendrik G. Kruger. (2013) Integrated Approach to Structure-Based Enzymatic Drug Design: Molecular Modeling, Spectroscopy, and Experimental Bioactivity. Chemical Reviews 114:1, pages 493-537.
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Xiuli Zhang, Huayun Deng, Yuansheng Xiao, Xingya Xue, Ann M. Ferrie, Elizabeth Tran, Xinmiao Liang & Ye Fang. (2014) Label-free cell phenotypic profiling identifies pharmacologically active compounds in two traditional Chinese medicinal plants. RSC Adv. 4:50, pages 26368-26377.
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Megan Morse, Haiyan Sun, Elizabeth Tran, Robert Levenson & Ye Fang. (2013) Label-free integrative pharmacology on-target of opioid ligands at the opioid receptor family. BMC Pharmacology and Toxicology 14:1.
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Dilsat Ozkan-AriksoysalYe Fang. 2013. Biosensors and their Applications in Healthcare. Biosensors and their Applications in Healthcare 54 67 .
Huayun Deng, Haiyan Sun & Ye Fang. (2013) Label-free cell phenotypic assessment of the biased agonism and efficacy of agonists at the endogenous muscarinic M3 receptors. Journal of Pharmacological and Toxicological Methods 68:3, pages 323-333.
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Natalya Zaytseva, Jeffery G. Lynn, Qi Wu, Deepti J. Mudaliar, Haiyan Sun, Patty Q. Kuang & Ye Fang. (2013) Resonant waveguide grating biosensor-enabled label-free and fluorescence detection of cell adhesion. Sensors and Actuators B: Chemical 188, pages 1064-1072.
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Huayun Deng & Ye Fang. (2013) The Three Catecholics Benserazide, Catechol and Pyrogallol are GPR35 Agonists. Pharmaceuticals 6:4, pages 500-509.
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Ye Fang. (2013) Troubleshooting and deconvoluting label-free cell phenotypic assays in drug discovery. Journal of Pharmacological and Toxicological Methods 67:2, pages 69-81.
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Huayun Deng, Chaoming Wang & Ye Fang. (2013) Label-free cell phenotypic assessment of the molecular mechanism of action of epidermal growth factor receptor inhibitors. RSC Advances 3:26, pages 10370.
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Ann M. Ferrie, Chaoming Wang, Huayun Deng & Ye Fang. (2013) A label-free optical biosensor with microfluidics identifies an intracellular signalling wave mediated through the β2-adrenergic receptor. Integrative Biology 5:10, pages 1253.
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Huayun Deng, Chaoming Wang, Ming Su & Ye Fang. (2012) Probing Biochemical Mechanisms of Action of Muscarinic M3 Receptor Antagonists with Label-Free Whole Cell Assays. Analytical Chemistry 84:19, pages 8232-8239.
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Huayun Deng & Ye Fang. (2012) Synthesis and Agonistic Activity at the GPR35 of 5,6-Dihydroxyindole-2-carboxylic Acid Analogues. ACS Medicinal Chemistry Letters 3:7, pages 550-554.
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Huayun Deng, Jieyu Hu, Haibei Hu, Mingqian He & Ye Fang. (2012) Thieno[3,2-b]thiophene-2-carboxylic acid derivatives as GPR35 agonists. Bioorganic & Medicinal Chemistry Letters 22:12, pages 4148-4152.
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Ann M. Ferrie, Oberon D. Deichmann, Qi Wu & Ye Fang. (2012) High resolution resonant waveguide grating imager for cell cluster analysis under physiological condition. Applied Physics Letters 100:22.
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Huayun Deng, Haibei Hu & Ye Fang. (2012) Multiple tyrosine metabolites are GPR35 agonists. Scientific Reports 2:1.
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Huayun Deng, Haibei Hu, Shizhang Ling, Ann M. Ferrie & Ye Fang. (2012) Discovery of Natural Phenols as G Protein-Coupled Receptor-35 (GPR35) Agonists. ACS Medicinal Chemistry Letters 3:2, pages 165-169.
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Huayun Deng & Ye Fang. (2012) Discovery of nitrophenols as GPR35 agonists. MedChemComm 3:10, pages 1270.
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