1,025
Views
50
CrossRef citations to date
0
Altmetric
Reviews

Fluorescence anisotropy (polarization): from drug screening to precision medicine

, & , PhD (Assistant Professor, Director Optical Spectroscopy Core Facility)

Keep up to date with the latest research on this topic with citation updates for this article.

Read on this site (1)

Asko Uri & Olivier Etebe Nonga. (2020) What is the current value of fluorescence polarization assays in small molecule screening?. Expert Opinion on Drug Discovery 15:2, pages 131-133.
Read now

Articles from other publishers (49)

Andrea Delledonne, Elisa Guazzelli, Silvia Pescina, Annalisa Bianchera, Giancarlo Galli, Elisa Martinelli & Cristina Sissa. (2023) Amphiphilic Fluorinated Unimer Micelles as Nanocarriers of Fluorescent Probes for Bioimaging. ACS Applied Nano Materials 6:17, pages 15551-15562.
Crossref
Peter R. Jermain, Dina H. Kandil, Alona Muzikansky, Ashraf Khan & Anna N. Yaroslavsky. (2023) Translational Potential of Fluorescence Polarization for Breast Cancer Cytopathology. Cancers 15:5, pages 1501.
Crossref
Jingrong Li, Boshi Liu, Li Liu, Nan Zhang, Yumeng Liao, Chunyu Zhao, Manzhu Cao, Yuxuan Zhong, Danni Chai, Xiaoyu Chen, Di Zhang, Haixia Wang, Yongzhi He & Zheng Li. (2023) Fluorescence-based aptasensors for small molecular food contaminants: From energy transfer to optical polarization. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 285, pages 121872.
Crossref
Sara Bobone, Claudia Storti, Paolo Calligari & Lorenzo Stella. 2023. SH2 Domains. SH2 Domains 93 112 .
Silva Khodjoyan, Deborha Morissette, Fortune Hontonnou, Luis Checa Ruano, Charles-Adrien Richard, Olivier Sperandio, Jean-François Eléouët, Marie Galloux, Philippe Durand, Stéphanie Deville-Foillard & Christina Sizun. (2022) Investigation of the Fuzzy Complex between RSV Nucleoprotein and Phosphoprotein to Optimize an Inhibition Assay by Fluorescence Polarization. International Journal of Molecular Sciences 24:1, pages 569.
Crossref
Zhou Chen, Sandra Zakrzewska, Holly S. Hajare, Aurora Alvarez-Buylla, Fayal Abderemane-Ali, Maximiliana Bogan, Dave Ramirez, Lauren A. O’Connell, J. Du Bois & Daniel L. MinorJr.Jr.. (2022) Definition of a saxitoxin (STX) binding code enables discovery and characterization of the anuran saxiphilin family. Proceedings of the National Academy of Sciences 119:44.
Crossref
Sonja Srdanović, Madita Wolter, Chi H. Trinh, Christian Ottmann, Stuart L. Warriner & Andrew J. Wilson. (2022) Understanding the interaction of 14‐3‐3 proteins with h DMX and h DM2: a structural and biophysical study . The FEBS Journal 289:17, pages 5341-5358.
Crossref
Sharon N. Greenwood, Regina G. Belz & Brian P. Weiser. (2022) A Conserved Mechanism for Hormesis in Molecular Systems. Dose-Response 20:3, pages 155932582211093.
Crossref
Riku Katsuki, Tsubasa Numayama, Yudai Tabuchi, Jaiyam Sharma, Naohito Satake, Adarsh Sandhu & Masumi Taki. (2022) Solvatochromic peptidic binder obtained via extended phage display acts as a fluororeporter for fragment-based drug discovery (FBDD). Analytical and Bioanalytical Chemistry 414:17, pages 4803-4807.
Crossref
Lei Wang, Chenxi Du, Hui Liu, Weimin Qiu, Xin Lu, Yanyu Hu, Yueqing Li, Tianyu Sun, Yao Chen & Haopeng Sun. (2022) A Practical and High‐Affinity Fluorescent Probe for Butyrylcholinesterase: A Good Strategy for Binding Affinity Characterization . Chinese Journal of Chemistry 40:11, pages 1285-1292.
Crossref
Christoph Müller, Jakob Gleixner, Maris-Johanna Tahk, Sergei Kopanchuk, Tõnis Laasfeld, Michael Weinhart, Dieter Schollmeyer, Martin U. Betschart, Steffen Lüdeke, Pierre Koch, Ago Rinken & Max Keller. (2022) Structure-Based Design of High-Affinity Fluorescent Probes for the Neuropeptide Y Y 1 Receptor . Journal of Medicinal Chemistry 65:6, pages 4832-4853.
Crossref
Yapiao Li, Hao Yu & Qiang Zhao. (2022) Aptamer fluorescence anisotropy assays for detection of aflatoxin B1 and adenosine triphosphate using antibody to amplify signal change. RSC Advances 12:12, pages 7464-7468.
Crossref
Xue Xiao & Shujun Zhen. (2022) Recent advances in fluorescence anisotropy/polarization signal amplification. RSC Advances 12:11, pages 6364-6376.
Crossref
Dairo A. Rendon & Juan L. Palacio. (2022) A modern, simple, and economical accessory for continuous L-format measurement of steady-state fluorescence anisotropy. Analytical Biochemistry 639, pages 114473.
Crossref
Gourab Prasad Pattnaik & Hirak Chakraborty. 2022. Optical Spectroscopic and Microscopic Techniques. Optical Spectroscopic and Microscopic Techniques 55 75 .
Sasha Padilla-Coley, Elley E. Rudebeck, Bradley D. Smith & Frederick M. Pfeffer. (2021) Intracellular fluorescence competition assay for inhibitor engagement of histone deacetylase. Bioorganic & Medicinal Chemistry Letters 47, pages 128207.
Crossref
Sudakshina Ganguly, N. Arul Murugan, Debasis Ghosh, Nagarjun Narayanaswamy, Thimmaiah Govindaraju & Gautam Basu. (2021) DNA Minor Groove-Induced cis – trans Isomerization of a Near-Infrared Fluorescent Probe . Biochemistry 60:26, pages 2084-2097.
Crossref
Maho Takioku, Yuta Takamura, Michiko Fujihara, Masaki Watanabe, Shoya Yamada, Mayu Kawasaki, Sohei Ito, Shogo Nakano & Hiroki Kakuta. (2021) Creation of Fluorescent RXR Antagonists Based on CBTF-EE and Application to a Fluorescence Polarization Binding Assay. ACS Medicinal Chemistry Letters 12:6, pages 1024-1029.
Crossref
Anna Wojtczak, Renata Kasprzyk, Marcin Warmiński, Krystian Ubych, Dorota Kubacka, Pawel J. Sikorski, Jacek Jemielity & Joanna Kowalska. (2021) Evaluation of carboxyfluorescein-labeled 7-methylguanine nucleotides as probes for studying cap-binding proteins by fluorescence anisotropy. Scientific Reports 11:1.
Crossref
Luiza de Carvalho Bertozo, Martyna Maszota-Zieleniak, Maytê Bolean, Pietro Ciancaglini, Sergey A. Samsonov & Valdecir F. Ximenes. (2021) Binding of fluorescent dansyl amino acids in albumin: When access to the protein cavity is more important than the strength of binding. Dyes and Pigments 188, pages 109195.
Crossref
Silvia Zorrilla, Begoña Monterroso, Miguel-Ángel Robles-Ramos, William Margolin & Germán Rivas. (2021) FtsZ Interactions and Biomolecular Condensates as Potential Targets for New Antibiotics. Antibiotics 10:3, pages 254.
Crossref
Jessica Colombo, Adrien Antkowiak, Konstantin Kogan, Tommi Kotila, Jenna Elliott, Audrey Guillotin, Pekka Lappalainen & Alphée Michelot. (2021) A functional family of fluorescent nucleotide analogues to investigate actin dynamics and energetics. Nature Communications 12:1.
Crossref
Clemens Schulte, Vladimir Khayenko, Noah Frieder Nordblom, Franziska Tippel, Violetta Peck, Amit Jean Gupta & Hans Michael Maric. (2021) High-throughput determination of protein affinities using unmodified peptide libraries in nanomolar scale. iScience 24:1, pages 101898.
Crossref
Jakub Stefaniak, Sébastien R. G. Galan & Kilian V. M. Huber. 2020. The Discovery and Utility of Chemical Probes in Target Discovery. The Discovery and Utility of Chemical Probes in Target Discovery 247 275 .
Vincent Blay, Bhairavi Tolani, Sunita P. Ho & Michelle R. Arkin. (2020) High-Throughput Screening: today’s biochemical and cell-based approaches. Drug Discovery Today 25:10, pages 1807-1821.
Crossref
Yunlong Bai, Tong Shu, Lei Su & Xueji Zhang. (2020) Functional nucleic acid-based fluorescence polarization/anisotropy biosensors for detection of biomarkers. Analytical and Bioanalytical Chemistry 412:25, pages 6655-6665.
Crossref
Isabelle Bertin-Jung, Anne Robert, Nick Ramalanjaona, Sandrine Gulberti, Catherine Bui, Jean-Baptiste Vincourt, Mohamed Ouzzine, Jean-Claude Jacquinet, Chrystel Lopin-Bon & Sylvie Fournel-Gigleux. (2020) A versatile strategy to synthesize N -methyl-anthranilic acid-labelled glycoprobes for fluorescence-based screening assays . Chemical Communications 56:73, pages 10746-10749.
Crossref
Qiang Zhao, Jeffrey Tao, Wei Feng, Jagdeesh S. Uppal, Hanyong Peng & X. Chris Le. (2020) Aptamer binding assays and molecular interaction studies using fluorescence anisotropy - A review. Analytica Chimica Acta 1125, pages 267-278.
Crossref
Angela Zapata, Mai-Loan Nguyen, Caleb Ling, Jacqueline Rogers, Sangeetha Domiano, Clive Hayzelden & Korin E. Wheeler. (2020) The role of human serum and solution chemistry in fibrinogen peptide–nanoparticle interactions. Nanoscale Advances 2:6, pages 2429-2440.
Crossref
Joel E. Adablah, Yao Wang, Matthew Donohue & Michael G. Roper. (2020) Profiling Glucose-Stimulated and M3 Receptor-Activated Insulin Secretion Dynamics from Islets of Langerhans Using an Extended-Lifetime Fluorescence Dye. Analytical Chemistry 92:12, pages 8464-8471.
Crossref
Bernardo Pereira Moreira, Tom Armstrong, Izabella Cristina Andrade Batista, Naiara Clemente Tavares, Camilla Valente Pires, Marina de Moraes Mourão, Franco H. Falcone & Lodewijk V. Dekker. (2020) Use of BODIPY-Labeled ATP Analogues in the Development and Validation of a Fluorescence Polarization-Based Assay for Screening of Kinase Inhibitors. ACS Omega 5:16, pages 9064-9070.
Crossref
Georgina Townshend, Gary S. Thompson, Lisa J. White, Jennifer R. Hiscock & Jose L. Ortega-Roldan. (2020) The elucidation of phospholipid bilayer–small molecule interactions using a combination of phospholipid nanodiscs and solution state NMR techniques. Chemical Communications 56:28, pages 4015-4018.
Crossref
Jungmi Lee, Baskaran Purushothaman & Joon Myong Song. (2019) Inkjet Bioprinting on Parchment Paper for Hit Identification from Small Molecule Libraries. ACS Omega 5:1, pages 588-596.
Crossref
Alexander P. DemchenkoAlexander P. Demchenko. 2020. Introduction to Fluorescence Sensing. Introduction to Fluorescence Sensing 31 54 .
V. B. Krylov, M. I. Petruk, M. P. Karimova, L. I. Mukhametova, A. L. Matveev, N. V. Tikunova, S. A. Eremin & N. E. Nifantiev. (2020) Potential of fluorescence polarization immunoassay for the detection of Aspergillus fumigatus galactomannan. Russian Chemical Bulletin 68:12, pages 2365-2369.
Crossref
Seong‐Hyun Park, Insu Shin, Sang‐Hyun Park, Nam Doo Kim & Injae Shin. (2019) An Inhibitor of the Interaction of Survivin with Smac in Mitochondria Promotes Apoptosis. Chemistry – An Asian Journal 14:22, pages 4035-4041.
Crossref
Claudio Vinegoni, Paolo Fumene Feruglio, Ignacy Gryczynski, Ralph Mazitschek & Ralph Weissleder. (2019) Fluorescence anisotropy imaging in drug discovery. Advanced Drug Delivery Reviews 151-152, pages 262-288.
Crossref
Seongho Park, Minju Kim, Jean-Claude Vial & Kwangseuk Kyhm. (2019) Stimulation Emission Depleted Photoacoustic. Optics Express 27:20, pages 27841.
Crossref
Aaron J. Wolfe, Kyle J. Parella & Liviu Movileanu. (2019) High‐Throughput Screening of Protein‐Detergent Complexes Using Fluorescence Polarization Spectroscopy. Current Protocols in Protein Science 97:1.
Crossref
Huiyan Zhang, Shupeng Yang, Karl De Ruyck, Natalia V. Beloglazova, Sergei A. Eremin, Sarah De Saeger, Suxia Zhang, Jianzhong Shen & Zhanhui Wang. (2019) Fluorescence polarization assays for chemical contaminants in food and environmental analyses. TrAC Trends in Analytical Chemistry 114, pages 293-313.
Crossref
Qiang Zhao, Jeffrey Tao, Jagdeesh S. Uppal, Hanyong Peng, Hailin Wang & X. Chris Le. (2019) Nucleic acid aptamers improving fluorescence anisotropy and fluorescence polarization assays for small molecules. TrAC Trends in Analytical Chemistry 110, pages 401-409.
Crossref
Aaron J. Wolfe, Jack F. Gugel, Min Chen & Liviu Movileanu. (2018) Kinetics of Membrane Protein–Detergent Interactions Depend on Protein Electrostatics. The Journal of Physical Chemistry B 122:41, pages 9471-9481.
Crossref
Nina I. Zolotarjova & Richard Wynn. (2018) Binding Assays for Bromodomain Proteins: Their Utility in Drug Discovery in Oncology and Inflammatory Disease. Current Protocols in Pharmacology 80:1, pages 3.16.1-3.16.14.
Crossref
Sheraz Gul. (2017) Epigenetic assays for chemical biology and drug discovery. Clinical Epigenetics 9:1.
Crossref
Aaron J. Wolfe, Wei Si, Zhengqi Zhang, Adam R. Blanden, Yi-Ching Hsueh, Jack F. Gugel, Bach Pham, Min Chen, Stewart N. Loh, Sharon Rozovsky, Aleksei Aksimentiev & Liviu Movileanu. (2017) Quantification of Membrane Protein-Detergent Complex Interactions. The Journal of Physical Chemistry B 121:44, pages 10228-10241.
Crossref
Crystal S. F. Cheung, Kyle W. Anderson, Pooja M. Patel, Keale L. Cade, Karen W. Phinney & Illarion V. Turko. (2017) A new approach to quantification of mAb aggregates using peptide affinity probes. Scientific Reports 7:1.
Crossref
Molly M. Lee & Blake R. Peterson. (2016) Quantification of Small Molecule–Protein Interactions using FRET between Tryptophan and the Pacific Blue Fluorophore. ACS Omega 1:6, pages 1266-1276.
Crossref
Matthew D Hall, Adam Yasgar, Tyler Peryea, John C Braisted, Ajit Jadhav, Anton Simeonov & Nathan P Coussens. (2016) Fluorescence polarization assays in high-throughput screening and drug discovery: a review. Methods and Applications in Fluorescence 4:2, pages 022001.
Crossref
Jae-Won Choi, Byung-Gwan Jo, Andrew J. deMello, Jaebum Choo & Hak Yong Kim. (2016) Streptavidin-triggered signal amplified fluorescence polarization for analysis of DNA–protein interactions. The Analyst 141:24, pages 6499-6502.
Crossref

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.