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Original Research

Detection of circulating microparticles by flow cytometry: influence of centrifugation, filtration of buffer, and freezing

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Pages 1125-1133 | Published online: 06 Dec 2010

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Morten Hjuler Nielsen, Henning Beck-Nielsen, Morten Nørgaard Andersen & Aase Handberg. (2014) A flow cytometric method for characterization of circulating cell-derived microparticles in plasma. Journal of Extracellular Vesicles 3:1.
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Vid Šuštar, Apolonija Bedina-Zavec, Roman Štukelj, Mojca Frank, Goran Bobojević, Rado Janša, Eva Ogorevc, Peter Kruljc, Keriya Mam, Boštjan Šimunič, Mateja Manček-Keber, Roman Jerala, Blaž Rozman, Peter Veranič, Henry Hägerstrand & Veronika Kralj-Iglič. (2011) Nanoparticles isolated from blood: a reflection of vesiculability of blood cells during the isolation process. International Journal of Nanomedicine 6, pages 2737-2748.
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T. V. Glazanova, E. R. Shilova, A. V. Chechetkin & L. N. Bubnova. (2021) Impact of transfusion of blood components on the recipient immune system. Medical Immunology (Russia) 23:6, pages 1307-1318.
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Michael C. Larson, Neil Hogg & Cheryl A. Hillery. (2021) Centrifugation Removes a Population of Large Vesicles, or “Macroparticles,” Intermediate in Size to RBCs and Microvesicles. International Journal of Molecular Sciences 22:3, pages 1243.
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Nima Abbasian, Karl E. Herbert, Izabella Pawluczyk, James O. Burton & Alan Bevington. (2018) Vesicles bearing gifts: the functional importance of micro-RNA transfer in extracellular vesicles in chronic kidney disease. American Journal of Physiology-Renal Physiology 315:5, pages F1430-F1443.
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Virtudes Vila-Liante, Verónica Sánchez-López, Vicenta Martínez-Sales, Luis A. Ramón-Nuñez, Elena Arellano-Orden, Alejandra Cano-Ruiz, Francisco J. Rodríguez-Martorell, Lin Gao & Remedios Otero-Candelera. (2016) Impact of sample processing on the measurement of circulating microparticles: storage and centrifugation parameters. Clinical Chemistry and Laboratory Medicine (CCLM) 54:11, pages 1759-1767.
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Michael Craig Larson, Cheryl A. Hillery & Neil Hogg. (2014) Circulating membrane-derived microvesicles in redox biology. Free Radical Biology and Medicine 73, pages 214-228.
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Bonnie K. Arendt, Denise K. Walters, Xiaosheng Wu, Renee C. Tschumper & Diane F. Jelinek. (2014) Multiple myeloma cell-derived microvesicles are enriched in CD147 expression and enhance tumor cell proliferation. Oncotarget 5:14, pages 5686-5699.
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Michael C. Larson, Maia R. Luthi, Neil Hogg & Cheryl A. Hillery. (2013) Calcium-phosphate microprecipitates mimic microparticles when examined with flow cytometry. Cytometry Part A 83A:2, pages 242-250.
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Walter G. Land. (2013) Transfusion-Related Acute Lung Injury: The Work of DAMPs*. Transfusion Medicine and Hemotherapy 40:1, pages 3-13.
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