126
Views
31
CrossRef citations to date
0
Altmetric
Original Article

Effects of different density gradient separation techniques on neutrophil function

, , , &
Pages 385-391 | Received 04 Oct 1993, Accepted 18 Mar 1994, Published online: 08 Jul 2009

Keep up to date with the latest research on this topic with citation updates for this article.

Read on this site (1)

Articles from other publishers (30)

Jiajia Jin, Hong Qian, Guannan Wu, Nirong Bao & Yong Song. (2020) Neutrophil-derived long noncoding RNA IL-7R predicts development of multiple organ dysfunction syndrome in patients with trauma. European Journal of Trauma and Emergency Surgery 48:3, pages 1545-1553.
Crossref
Alex Quach & Antonio Ferrante. (2017) The Application of Dextran Sedimentation as an Initial Step in Neutrophil Purification Promotes Their Stimulation, due to the Presence of Monocytes. Journal of Immunology Research 2017, pages 1-10.
Crossref
Jing-Bo Wang, Yan-Jun Zhang, Juan Guan, Li Zhou, Yu Sheng, Yan Zhang & Yan-Fang Si. (2011) Enhanced syndecan-1 expression on neutrophils in patients with type 2 diabetes mellitus. Acta Diabetologica 49:1, pages 41-46.
Crossref
Virginie Pascal, Brice Laffleur & Michel Cogné. 2012. Antibody Methods and Protocols. Antibody Methods and Protocols 295 317 .
Rabindra Tirouvanziam, Daisy Diaz, Yael Gernez, Julie Laval, Monique Crubezy & Megha Makam. 2011. Advanced Optical Flow Cytometry. Advanced Optical Flow Cytometry 333 362 .
Alan Strassburg, Arne Luers & Klaus Dalhoff. (2010) Decreased apoptosis of pulmonary PMN in COPD patients with community‐acquired pneumonia. The Clinical Respiratory Journal 4:2, pages 111-119.
Crossref
Jolanta Sroka, Anna Kordecka, Przemysław Włosiak, Zbigniew Madeja & Włodzimierz Korohoda. (2009) Separation methods for isolation of human polymorphonuclear leukocytes affect their motile activity. European Journal of Cell Biology 88:9, pages 531-539.
Crossref
Xixing Zhao, Jennifer R. Town, Fang Li, Xiaobei Zhang, Donald W. Cockcroft & John R. Gordon. (2009) ELR-CXC Chemokine Receptor Antagonism Targets Inflammatory Responses at Multiple Levels. The Journal of Immunology 182:5, pages 3213-3222.
Crossref
Maura Farinacci, Monica Colitti, Sandy Sgorlon & Bruno Stefanon. (2008) Immunomodulatory activity of plant residues on ovine neutrophils. Veterinary Immunology and Immunopathology 126:1-2, pages 54-63.
Crossref
Yoshihiro Mitani, Keizo Sato, Yosuke Muramoto, Tomohiro Karakawa, Masataka Kitamado, Tatsuya Iwanaga, Tetsuji Nabeshima, Kumiko Maruyama, Kazuko Nakagawa, Kazuhiko Ishida & Kazumi Sasamoto. (2008) Superoxide scavenging activity of pirfenidone–iron complex. Biochemical and Biophysical Research Communications 372:1, pages 19-23.
Crossref
Makoto NAKATA, Takuya ITOU & Takeo SAKAI. (2007) Isolation and Chemiluminescent Properties of Ferret (Mustela putorius furo) Polymorphonuclear Cells. Journal of Veterinary Medical Science 69:12, pages 1321-1324.
Crossref
Rabindra Tirouvanziam, Carol K. Conrad, Teodoro Bottiglieri, Leonore A. Herzenberg, Richard B. Moss & Leonard A. Herzenberg. (2006) High-dose oral N -acetylcysteine, a glutathione prodrug, modulates inflammation in cystic fibrosis . Proceedings of the National Academy of Sciences 103:12, pages 4628-4633.
Crossref
Deborah S. Goddard, Kei-ichi Yamanaka, Thomas S. Kupper & David A. Jones. (2005) Activation of Neutrophils in Cutaneous T-Cell Lymphoma. Clinical Cancer Research 11:23, pages 8243-8249.
Crossref
Rudolf Richter, Roxana Bistrian, Sylvia Escher, Wolf-Georg Forssmann, Jalal Vakili, Reinhard Henschler, Nikolaj Spodsberg, Adjoa Frimpong-Boateng & Ulf Forssmann. (2005) Quantum Proteolytic Activation of Chemokine CCL15 by Neutrophil Granulocytes Modulates Mononuclear Cell Adhesiveness. The Journal of Immunology 175:3, pages 1599-1608.
Crossref
Birgit Kraft, Winfried Wintersberger & Hans G Kress. (2004) Cannabinoid receptor-independent suppression of the superoxide generation of human neutrophils (PMN) by CP55 940, but not by anandamide. Life Sciences 75:8, pages 969-977.
Crossref
W REINISCH, C LICHTENBERGER, G STEGER, W TILLINGER, O SCHEINER, A GANGL, D MAURER & M WILLHEIM. (2003) Donor dependent, interferon- γ induced HLA-DR expression on human neutrophils in vivo . Clinical and Experimental Immunology 133:3, pages 476-484.
Crossref
S Comazzi, S Paltrinieri, V Spagnolo & P Sartorelli. (2001) Evaluation In Vitro of Canine Neutrophil Function. The Veterinary Journal 162:3, pages 219-225.
Crossref
S. Paltrinieri, S. Panelli & P. Sartorelli. (2001) Characteristics of the response of ovine granulocytes (PMNs) to zymosan‐activated serum (ZAS) and to recombinant human interleukin‐8 (IL‐8). Journal of Veterinary Medicine Series A 47:7, pages 421-430.
Crossref
P.M Kern, M Herrmann, B Stockmeyer, J.R Kalden, T Valerius & R Repp. (2000) Flow cytometric discrimination between viable neutrophils, apoptotic neutrophils and eosinophils by double labelling of permeabilized blood granulocytes. Journal of Immunological Methods 241:1-2, pages 11-18.
Crossref
Patrizia Scapini, Federica Calzetti & Marco A Cassatella. (1999) On the detection of neutrophil-derived vascular endothelial growth factor (VEGF). Journal of Immunological Methods 232:1-2, pages 121-129.
Crossref
N Vachi?ry, P Tott?V Balcer, D Martinez & A Bensaid. (1999) Effect of isolation techniques, in vitro culture and IFN? treatment on the constitutive expression of MHC Class I and Class II molecules on goat neutrophils. Veterinary Immunology and Immunopathology 70:1-2, pages 19-32.
Crossref
Cornelia Lichtenberger, Schaker Zakeri, Karin Baier, Martin Willheim, Margareta Holub & Walter Reinisch. (1999) A novel high-purity isolation method for human peripheral blood neutrophils permitting polymerase chain reaction-based mRNA studies. Journal of Immunological Methods 227:1-2, pages 75-84.
Crossref
Michael Heinzelmann, Sarah Appel Gardner, Mark Mercer‐Jones, Amy J. Roll & Hiram C. PolkJr.Jr.. (2013) Quantification of Phagocytosis in Human Neutrophils by Flow Cytometry. Microbiology and Immunology 43:6, pages 505-512.
Crossref
M Fredriksson, A Gustafsson, B. Åsman & K Bergström. (2008) Periodontitis increases chemiluminescence of the peripheral neutrophils independently of priming by the preparation method. Oral Diseases 5:3, pages 229-233.
Crossref
Joern Heine, Dirk Scheinichen, Karsten Jaeger, Thomas Herzog, Robert Sümpelmann & Martin Leuwer. (1999) Effect of magnetic resonance imaging on human respiratory burst of neutrophils. FEBS Letters 446:1, pages 15-17.
Crossref
Marco Antonio Cassatella. 1999. 369 509 .
Tohru Takada, Takeshi Kinkori, Hisako Muramatsu, Akemi Hayakawa, Shuhei Torii & Takashi Muramatsu. (1997) Midkine, a Retinoic Acid-Inducible Heparin-Binding Cytokine, Is a Novel Regulator of Intracellular Calcium in Human Neutrophils. Biochemical and Biophysical Research Communications 241:3, pages 756-761.
Crossref
F Souques, Ch Duperray, J P?ne, J Bousquet & B Arnoux. (1997) Modification of surface marker expression on CD14 monocytes of allergic patients after lysis or Ficoll purification. Journal of Immunological Methods 204:2, pages 153-160.
Crossref
Masamichi Mineshita, Toshiki Kimura, Hiroshi Murai, Chie Moritani, Shinichi Ishioka, Masayuki Kambe & Michio Yamakido. (1997) Whole-blood incubation method to study neutrophil cytoskeletal dynamics. Journal of Immunological Methods 202:1, pages 59-66.
Crossref
Stefan Zahler, Christian Kowalski, Andreas Brosig, Christian Kupatt, Bernhard F. Becker & Eckehart Gerlach. (1997) The function of neutrophils isolated by a magnetic antibody cell separation technique is not altered in comparison to a density gradient centrifugation method. Journal of Immunological Methods 200:1-2, pages 173-179.
Crossref

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.