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Connective Tissue Research Volume 52, 2011 - Issue 3
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Research Article

Isolation and expansion of synovial CD34−CD44+CD90+ mesenchymal stem cells: comparison of an enzymatic method and a direct explant technique

Dae-Hee LeeDepartment of Orthopaedic Surgery, Korea University College of Medicine, Seoul, Korea
,
Seong-Dae JooDepartment of Orthopaedic Surgery, Korea University College of Medicine, Seoul, Korea
,
Seung-Beom HanDepartment of Orthopaedic Surgery, Korea University College of Medicine, Seoul, Korea
,
Jisu ImDepartment of Orthopaedic Surgery, Korea University College of Medicine, Seoul, Korea
,
Soon-Hyuck LeeDepartment of Orthopaedic Surgery, Korea University College of Medicine, Seoul, KoreaCorrespondence[email protected] [email protected]
,
Chung Hee SonnDepartment of Biochemistry and Molecular Biology, Division of Brain Korea 21 Program for Biomedical Science, Korea University College of Medicine, Seoul, Korea
&
Kyung-Mi LeeDepartment of Biochemistry and Molecular Biology, Division of Brain Korea 21 Program for Biomedical Science, Korea University College of Medicine, Seoul, KoreaCorrespondence[email protected] [email protected]
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Pages 226-234 | Received 22 Jun 2010, Accepted 16 Aug 2010, Published online: 30 Nov 2010

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V. P. Mantripragada, N. S. Piuzzi, W. A. Bova, C. Boehm, N. A. Obuchowski, V. Lefebvre, R. J. Midura & G. F. Muschler. (2019) Donor-matched comparison of chondrogenic progenitors resident in human infrapatellar fat pad, synovium, and periosteum - implications for cartilage repair. Connective Tissue Research 60:6, pages 597-610.
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Koushan Sineh Sepehr, Alireza Razavi, Mohsen Saeidi, Majid Mossahebi-Mohammadi, Meghdad Abdollahpour-Alitappeh & Seyed Mahmoud Hashemi. (2018) Development of a novel explant culture method for the isolation of mesenchymal stem cells from human breast tumor. Journal of Immunoassay and Immunochemistry 39:2, pages 207-217.
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Articles from other publishers (21)

Hannah J. Stage, Susanne Trappe, Katharina Söllig, Dagmar S. Trachsel, Katharina Kirsch, Cornelia Zieger, Roswitha Merle, Jörg R. Aschenbach & Heidrun Gehlen. (2023) Multilineage Differentiation Potential of Equine Adipose-Derived Stromal/Stem Cells from Different Sources. Animals 13:8, pages 1352.
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Ryota Chijimatsu, Taiga Takeda, Shinsaku Tsuji, Kohei Sasaki, Koichi Kato, Rie Kojima, Noriko Michihata, Toshiya Tsubaki, Aya Matui, Miharu Watanabe, Sakae Tanaka & Taku Saito. (2022) Development of hydroxyapatite-coated nonwovens for efficient isolation of somatic stem cells from adipose tissues. Regenerative Therapy 21, pages 52-61.
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Dagmar S. Trachsel, Hannah J. Stage, Sebastian Rausch, Susanne Trappe, Katharina Söllig, Gerhard Sponder, Roswitha Merle, Jörg R. Aschenbach & Heidrun Gehlen. (2022) Comparison of Sources and Methods for the Isolation of Equine Adipose Tissue-Derived Stromal/Stem Cells and Preliminary Results on Their Reaction to Incubation with 5-Azacytidine. Animals 12:16, pages 2049.
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Adrienne Wright, Marne L. Arthaud-Day & Mark L. Weiss. (2021) Therapeutic Use of Mesenchymal Stromal Cells: The Need for Inclusive Characterization Guidelines to Accommodate All Tissue Sources and Species. Frontiers in Cell and Developmental Biology 9.
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Ming-Yen Hsiao, Ping-Cheng Lin, Wei-Hao Liao, Wen-Shiang Chen, Chia-Hsien Hsu, Cheng-Kun He, Ya-Wen Wu, Amit Gefen, Michele Iafisco, Lixin Liu & Feng-Huei Lin. (2019) The Effect of the Repression of Oxidative Stress on Tenocyte Differentiation: A Preliminary Study of a Rat Cell Model Using a Novel Differential Tensile Strain Bioreactor. International Journal of Molecular Sciences 20:14, pages 3437.
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Yi Lin, Mayumi Umebayashi, Mohamed-Nur Abdallah, Guoying Dong, Michael G. Roskies, Yaoyao Fiona Zhao, Monzur Murshed, Zhiguang Zhang & Simon D. Tran. (2019) Combination of polyetherketoneketone scaffold and human mesenchymal stem cells from temporomandibular joint synovial fluid enhances bone regeneration. Scientific Reports 9:1.
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Norihiko Sugita, Yu Moriguchi, Morito Sakaue, David A. Hart, Yukihiko Yasui, Kota Koizumi, Ryota Chijimatsu, Syoichi Shimomura, Yasutoshi Ikeda, Hideki Yoshikawa & Norimasa Nakamura. (2016) Optimization of human mesenchymal stem cell isolation from synovial membrane: Implications for subsequent tissue engineering effectiveness. Regenerative Therapy 5, pages 79-85.
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António M. de Soure, Ana Fernandes-Platzgummer, Cláudia L. da Silva & Joaquim M.S. Cabral. (2016) Scalable microcarrier-based manufacturing of mesenchymal stem/stromal cells. Journal of Biotechnology 236, pages 88-109.
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Ching-Wen Tsai & Tai-Horng Young. (2016) CD44 expression trends of mesenchymal stem-derived cell, cancer cell and fibroblast spheroids on chitosan-coated surfaces. Pure and Applied Chemistry 88:9, pages 843-852.
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Niina Hopper, Frances Henson, Roger Brooks, Erden Ali, Neil Rushton & John Wardale. (2015) Peripheral blood derived mononuclear cells enhance osteoarthritic human chondrocyte migration. Arthritis Research & Therapy 17:1.
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Seong-Seo NohSuk Ho BhangWan-Geun LaSeahyoung LeeJung-Youn ShinYoon-Ji MaHyeon-Ki JangSeokyung KangMin JinJooyeon ParkByung-Soo Kim. (2015) A Dual Delivery of Substance P and Bone Morphogenetic Protein-2 for Mesenchymal Stem Cell Recruitment and Bone Regeneration. Tissue Engineering Part A 21:7-8, pages 1275-1287.
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Dae-Hee Lee, Joanne Ng, Sang-Beom Kim, Chung Hee Sonn, Kyung-Mi Lee & Seung-Beom Han. (2015) Effect of Donor Age on the Proportion of Mesenchymal Stem Cells Derived from Anterior Cruciate Ligaments. PLOS ONE 10:3, pages e0117224.
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Santiago Roura, Josep Maria Pujal, Carolina Gálvez-Montón & Antoni Bayes-Genis. (2015) Impact of Umbilical Cord Blood-Derived Mesenchymal Stem Cells on Cardiovascular Research. BioMed Research International 2015, pages 1-6.
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Junfang Wu, Jie Niu, Xiaopeng Li, Xianwei Wang, Zhikun Guo & Fenxi Zhang. (2014) TGF-β1 induces senescence of bone marrow mesenchymal stem cells via increase of mitochondrial ROS production. BMC Developmental Biology 14:1.
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M. C. Valadares, J. P. Gomes, G. Castello, A. Assoni, M. Pellati, C. Bueno, M. Corselli, H. Silva, P. Bartolini, M. Vainzof, P. F. Margarido, E. Baracat, B. Péault & M. Zatz. (2014) Human Adipose Tissue Derived Pericytes Increase Life Span in Utrn tm1Ked Dmd mdx /J Mice. Stem Cell Reviews and Reports 10:6, pages 830-840.
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Hélène BusserCécile De BruynFrédéric UrbainMehdi NajarKarlien PietersGordana RaicevicNathalie MeulemanDominique BronLaurence Lagneaux. (2014) Isolation of Adipose-Derived Stromal Cells Without Enzymatic Treatment: Expansion, Phenotypical, and Functional Characterization. Stem Cells and Development 23:19, pages 2390-2400.
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Nancy Priya, Shilpita Sarcar, Anish Sen Majumdar & Swathi SundarRaj. (2014) Explant culture: a simple, reproducible, efficient and economic technique for isolation of mesenchymal stromal cells from human adipose tissue and lipoaspirate. Journal of Tissue Engineering and Regenerative Medicine 8:9, pages 706-716.
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P. Pastides, M. Chimutengwende-Gordon, N. Maffulli & W. Khan. (2013) Stem cell therapy for human cartilage defects: a systematic review. Osteoarthritis and Cartilage 21:5, pages 646-654.
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Claudia Gittel, Walter Brehm, Janina Burk, Henriette Juelke, Carsten Staszyk & Iris Ribitsch. (2013) Isolation of equine multipotent mesenchymal stromal cells by enzymatic tissue digestion or explant technique: comparison of cellular properties. BMC Veterinary Research 9:1, pages 221.
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Yvonne Peck & Dong-An Wang. 2013. Emerging Trends in Cell and Gene Therapy. Emerging Trends in Cell and Gene Therapy 373 409 .
D.-H. Lee, C.H. Sonn, S.-B. Han, Y. Oh, K.-M. Lee & S.-H. Lee. (2012) Synovial fluid CD34− CD44+ CD90+ mesenchymal stem cell levels are associated with the severity of primary knee osteoarthritis. Osteoarthritis and Cartilage 20:2, pages 106-109.
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