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Molecular Membrane Biology Volume 28, 2011 - Issue 6
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Research Article

Improving recombinant eukaryotic membrane protein yields in Pichia pastoris: The importance of codon optimization and clone selection

Fredrik ÖbergDepartment of Chemistry/Biochemistry, University of Gothenburg, Göteborg, Sweden
,
Jennie SjöhamnDepartment of Chemistry/Biochemistry, University of Gothenburg, Göteborg, Sweden
,
Matthew T. Conner School of Life and Health Sciences, Aston University, Birmingham, UK
,
Roslyn M. Bill School of Life and Health Sciences, Aston University, Birmingham, UK
&
Kristina HedfalkDepartment of Chemistry/Biochemistry, University of Gothenburg, Göteborg, SwedenCorrespondence[email protected]
Pages 398-411 | Received 24 Jun 2011, Accepted 27 Jun 2011, Published online: 19 Jul 2011

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Komal Ghauri, Hazrat Ali, Nayla Munawar, Muhammad Afzal Ghauri & Munir Ahmad Anwar. (2021) Glycoside hydrolase family 68 gene of halophilic archaeon Halalkalicoccus jeotgali B3T codes for an inulosucrase enzyme. Biocatalysis and Biotransformation 39:3, pages 206-213.
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Kristina Hedfalk. (2013) Further advances in the production of membrane proteins in . Bioengineered 4:6, pages 363-367.
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Fredrik Öberg & Kristina Hedfalk. (2013) Recombinant production of the human aquaporins in the yeast Pichia pastoris (Invited Review). Molecular Membrane Biology 30:1, pages 15-31.
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Jiao Zeng, Florian Schmitz, Simon Isaksson, Jessica Glas, Olivia Arbab, Martin Andersson, Kristina Sundell, Leif A Eriksson, Kunchithapadam Swaminathan, Susanna Törnroth-Horsefield & Kristina Hedfalk. (2022) High-resolution structure of a fish aquaporin reveals a novel extracellular fold. Life Science Alliance 5:12, pages e202201491.
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Antonio Carlesso, Raquel Delgado, Oriol Ruiz Isant, Owens Uwangue, Dylan Valli, Roslyn M Bill & Kristina Hedfalk. (2022) Yeast as a tool for membrane protein production and structure determination. FEMS Yeast Research 22:1.
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Tamim Al-Jubair, Jonas Hyld Steffen, Julie Winkel Missel, Philip Kitchen, Mootaz M. Salman, Roslyn M. Bill, Pontus Gourdon & Susanna Törnroth-Horsefield. (2022) High-yield overproduction and purification of human aquaporins from Pichia pastoris. STAR Protocols 3:2, pages 101298.
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Mariafrancesca Scalise, Gilda Pappacoda, Tiziano Mazza, Lara Console, Lorena Pochini & Cesare Indiveri. (2022) Cysteine 467 of the ASCT2 Amino Acid Transporter Is a Molecular Determinant of the Antiport Mechanism. International Journal of Molecular Sciences 23:3, pages 1127.
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Yongchao Mei, Xiuqing Li, Bo Yang, Jianxin Zhao, Hao Zhang, Haiqin Chen & Wei Chen. (2022) Heterologous expression of a novel linoleic acid isomerase BBI, and effect of fusion tags on its performance. Current Research in Food Science 5, pages 2053-2060.
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Jie LiSam A. BazziFlorian SchmitzHidetaka TannoJonathan R. McDaniel, Chang-Han LeeChaitanya JoshiJin Eyun KimNancy MonsonBenjamin M. GreenbergKristina HedfalkEsther MelamedGregory C. Ippolito. (2021) Molecular Level Characterization of Circulating Aquaporin-4 Antibodies in Neuromyelitis Optica Spectrum Disorder. Neurology Neuroimmunology & Neuroinflammation 8:5.
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Philip Kitchen, Mootaz M. Salman, Andrea M. Halsey, Charlotte Clarke-Bland, Justin A. MacDonald, Hiroaki Ishida, Hans J. Vogel, Sharif Almutiri, Ann Logan, Stefan Kreida, Tamim Al-Jubair, Julie Winkel Missel, Pontus Gourdon, Susanna Törnroth-Horsefield, Matthew T. Conner, Zubair Ahmed, Alex C. Conner & Roslyn M. Bill. (2020) Targeting Aquaporin-4 Subcellular Localization to Treat Central Nervous System Edema. Cell 181:4, pages 784-799.e19.
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Rachel Munro, Jeffrey de Vlugt, Vladimir Ladizhansky & Leonid S. Brown. (2020) Improved Protocol for the Production of the Low-Expression Eukaryotic Membrane Protein Human Aquaporin 2 in Pichia pastoris for Solid-State NMR. Biomolecules 10:3, pages 434.
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Hao Wang, Stefan Schoebel, Florian Schmitz, Hansong Dong & Kristina Hedfalk. (2020) Characterization of aquaporin-driven hydrogen peroxide transport. Biochimica et Biophysica Acta (BBA) - Biomembranes 1862:2, pages 183065.
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Mariafrancesca Scalise, Lorena Pochini, Jessica Cosco, Emma Aloe, Tiziano Mazza, Lara Console, Antonella Esposito & Cesare Indiveri. (2019) Interaction of Cholesterol With the Human SLC1A5 (ASCT2): Insights Into Structure/Function Relationships. Frontiers in Molecular Biosciences 6.
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Sara Arbulu, Juan J. Jiménez, Loreto Gútiez, Javier Feito, Luis M. Cintas, Carmen Herranz & Pablo E. Hernández. (2019) Cloning and expression of synthetic genes encoding native, hybrid- and bacteriocin-derived chimeras from mature class IIa bacteriocins, by Pichia pastoris (syn. Komagataella spp.). Food Research International 121, pages 888-899.
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Mariafrancesca Scalise, Lorena Pochini, Lara Console, Gilda Pappacoda, Piero Pingitore, Kristina Hedfalk & Cesare Indiveri. (2018) Cys Site-Directed Mutagenesis of the Human SLC1A5 (ASCT2) Transporter: Structure/Function Relationships and Crucial Role of Cys467 for Redox Sensing and Glutamine Transport. International Journal of Molecular Sciences 19:3, pages 648.
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Huihui Xu, Kunyuan Tie, Yang Zhang, Xin Feng, Yuan Cao & Wenyu Han. (2018) Design, expression, and characterization of the hybrid antimicrobial peptide T-catesbeianin-1 based on FyuA. Journal of Peptide Science 24:1, pages e3059.
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Jan-Philipp Schwarzhans, Tobias Luttermann, Martina Geier, Jörn Kalinowski & Karl Friehs. (2017) Towards systems metabolic engineering in Pichia pastoris. Biotechnology Advances 35:6, pages 681-710.
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Hanzhen Qiao, Wenfei Zhang, Wutai Guan, Fang Chen, Shihai Zhang & Zixiao Deng. (2017) Enhanced expression of lipase I from Galactomyces geotrichum by codon optimisation in Pichia pastoris. Protein Expression and Purification 138, pages 34-45.
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Etienne Rebuffet, Anna Frick, Michael Järvå & Susanna Törnroth-Horsefield. (2017) Cell-free production and characterisation of human uncoupling protein 1–3. Biochemistry and Biophysics Reports 10, pages 276-281.
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A. S. Dolgova & O. A. Stukolova. (2017) High-fidelity PCR enzyme with DNA-binding domain facilitates de novo gene synthesis. 3 Biotech 7:2.
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Simon Isaksson, Erik B. Watkins, Kathryn L. Browning, Tania Kjellerup Lind, Marité Cárdenas, Kristina Hedfalk, Fredrik Höök & Martin Andersson. (2016) Protein-Containing Lipid Bilayers Intercalated with Size-Matched Mesoporous Silica Thin Films. Nano Letters 17:1, pages 476-485.
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Zhenyu Hao, Maren Thomsen, Vincent L. G. Postis, Amelia Lesiuk, David Sharples, Yingying Wang, Mark Bartlam & Adrian Goldman. (2016) A Novel and Fast Purification Method for Nucleoside Transporters. Frontiers in Molecular Biosciences 3.
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Xu Li, Zimin Liu, Guilong Wang, Dujie Pan, Liangcheng Jiao & Yunjun Yan. (2016) Overexpression of Candida rugosa lipase Lip1 via combined strategies in Pichia pastoris. Enzyme and Microbial Technology 82, pages 115-124.
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Philip Kitchen, Fredrik ?berg, Jennie Sj?hamn, Kristina Hedfalk, Roslyn M. Bill, Alex C. Conner, Matthew T. Conner & Susanna T?rnroth-Horsefield. (2015) Plasma Membrane Abundance of Human Aquaporin 5 Is Dynamically Regulated by Multiple Pathways. PLOS ONE 10:11, pages e0143027.
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Janet To & Jaume Torres. (2015) Can Stabilization and Inhibition of Aquaporins Contribute to Future Development of Biomimetic Membranes?. Membranes 5:3, pages 352-368.
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Huikun Duan, Haisong Wang, Baicheng Ma, Pingzhe Jiang, Peipei Tu, Zaizhong Ni, Xiaodan Li, Miao Li, Xiaofeng Ma, Bin Wang, Ri Wu & Minggang Li. (2015) Codon optimization and expression of irisin in Pichia pastoris GS115. International Journal of Biological Macromolecules 79, pages 21-26.
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Wen-Jing Zhou, Jiang-Ke Yang, Lin Mao & Li-Hong Miao. (2015) Codon optimization, promoter and expression system selection that achieved high-level production of Yarrowia lipolytica lipase in Pichia pastoris. Enzyme and Microbial Technology 71, pages 66-72.
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Roslyn M Bill. (2015) Recombinant protein subunit vaccine synthesis in microbes: a role for yeast?. Journal of Pharmacy and Pharmacology 67:3, pages 319-328.
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Sara Arbulu, Juan J. Jim?nez, Loreto G?tiez, Luis M. Cintas, Carmen Herranz & Pablo E. Hern?ndez. (2015) Cloning and Expression of Synthetic Genes Encoding the Broad Antimicrobial Spectrum Bacteriocins SRCAM 602, OR-7, E-760, and L-1077, by Recombinant Pichia pastoris . BioMed Research International 2015, pages 1-11.
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Thomas Vogl, Gerhard G. Thallinger, Guenther Zellnig, David Drew, James M. Cregg, Anton Glieder & Maria Freigassner. (2014) Towards improved membrane protein production in Pichia pastoris: General and specific transcriptional response to membrane protein overexpression. New Biotechnology 31:6, pages 538-552.
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Anita Emmerstorfer, Tamara Wriessnegger, Melanie Hirz & Harald Pichler. (2014) Overexpression of membrane proteins from higher eukaryotes in yeasts. Applied Microbiology and Biotechnology 98:18, pages 7671-7698.
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Juan J. Jim?nez, Juan Borrero, Loreto G?tiez, Sara Arbulu, Carmen Herranz, Luis M. Cintas & Pablo E. Hern?ndez. (2014) Use of Synthetic Genes for Cloning, Production and Functional Expression of the Bacteriocins Enterocin A and Bacteriocin E 50-52 by Pichia pastoris and Kluyveromyces lactis. Molecular Biotechnology 56:6, pages 571-583.
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Jie-Xian Dong, Xi Xie, Da-Wei Hu, Shu-Chi Chen, Yong-Sheng He, Ross C. Beier, Yu-Dong Shen, Yuan-Ming Sun, Zhen-Lin Xu, Hong Wang & Jin-Yi Yang. (2013) Codon modification for the DNA sequence of a single-chain Fv antibody against clenbuterol and expression in Pichia pastoris. Applied Microbiology and Biotechnology 98:8, pages 3679-3689.
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Pankaj Panwar & M. Joanne Lemieux. (2014) Expression and Purification of Haemophilus influenzae Rhomboid Intramembrane Protease GlpG for Structural Studies . Current Protocols in Protein Science 76:1.
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Roslyn M. Bill. (2014) Playing catch-up with Escherichia coli: using yeast to increase success rates in recombinant protein production experiments. Frontiers in Microbiology 5.
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Piero Pingitore, Lorena Pochini, Mariafrancesca Scalise, Michele Galluccio, Kristina Hedfalk & Cesare Indiveri. (2013) Large scale production of the active human ASCT2 (SLC1A5) transporter in Pichia pastoris — functional and kinetic asymmetry revealed in proteoliposomes. Biochimica et Biophysica Acta (BBA) - Biomembranes 1828:9, pages 2238-2246.
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Man Wang, Shuai Jiang, Xiaoying Liu & Yefu Wang. (2013) Expression, purification, and immunogenic characterization of Epstein–Barr virus recombinant EBNA1 protein in Pichia pastoris. Applied Microbiology and Biotechnology 97:14, pages 6251-6262.
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Sanaz Emami, Ying Fan, Rachel Munro, Vladimir Ladizhansky & Leonid S. Brown. (2013) Yeast-expressed human membrane protein aquaporin-1 yields excellent resolution of solid-state MAS NMR spectra. Journal of Biomolecular NMR 55:2, pages 147-155.
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Jiang-Ke Yang, Li-Ying Liu, Jiang-Hong Dai & Qin Li. (2013) de novo Design and Synthesis of Candida antarctica Lipase B Gene and α-Factor Leads to High-Level Expression in Pichia pastoris. PLoS ONE 8:1, pages e53939.
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Jiang-Ke Yang, Fang-Yuan Chen, Xiang-Xiang Yan, Li-Hong Miao & Jiang-Hong Dai. (2012) A Simple and Accurate Two-Step Long DNA Sequences Synthesis Strategy to Improve Heterologous Gene Expression in Pichia. PLoS ONE 7:5, pages e36607.
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Nagamani Bora. 2012. Recombinant Protein Production in Yeast. Recombinant Protein Production in Yeast 217 235 .

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