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Research Article

Recombinant production of the human aquaporins in the yeast Pichia pastoris (Invited Review)

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Pages 15-31 | Received 16 Dec 2011, Accepted 06 Feb 2012, Published online: 21 Aug 2012

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Kristina Hedfalk. (2013) Further advances in the production of membrane proteins in . Bioengineered 4:6, pages 363-367.
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Articles from other publishers (19)

Antonio Carlesso, Raquel Delgado, Oriol Ruiz Isant, Owens Uwangue, Dylan Valli, Roslyn M Bill & Kristina Hedfalk. (2022) Yeast as a tool for membrane protein production and structure determination. FEMS Yeast Research 22:1.
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Roslyn M. Bill & Kristina Hedfalk. (2021) Aquaporins – Expression, purification and characterization. Biochimica et Biophysica Acta (BBA) - Biomembranes 1863:9, pages 183650.
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Douglas B. Kell. 2021. 1 177 .
Rachel Munro, Jeffrey de Vlugt, Vladimir Ladizhansky & Leonid S. Brown. (2020) Improved Protocol for the Production of the Low-Expression Eukaryotic Membrane Protein Human Aquaporin 2 in Pichia pastoris for Solid-State NMR. Biomolecules 10:3, pages 434.
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Hao Wang, Stefan Schoebel, Florian Schmitz, Hansong Dong & Kristina Hedfalk. (2020) Characterization of aquaporin-driven hydrogen peroxide transport. Biochimica et Biophysica Acta (BBA) - Biomembranes 1862:2, pages 183065.
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Frederik Bühring Bjørkskov, Simon Lyngaa Krabbe, Casper Normann Nurup, Julie Winkel Missel, Mariana Spulber, Julie Bomholt, Karen Molbaek, Claus Helix-Nielsen, Kamil Gotfryd, Pontus Gourdon & Per Amstrup Pedersen. (2017) Purification and functional comparison of nine human Aquaporins produced in Saccharomyces cerevisiae for the purpose of biophysical characterization. Scientific Reports 7:1.
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Henry Ampah-Korsah, Yonathan Sonntag, Angelica Engfors, Andreas Kirscht, Per Kjellbom & Urban Johanson. (2017) Single amino acid substitutions in the selectivity filter render NbXIP1;1α aquaporin water permeable. BMC Plant Biology 17:1.
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Thierry O. Wambo, Roberto A. Rodriguez & Liao Y. Chen. (2017) Computing osmotic permeabilities of aquaporins AQP4, AQP5, and GlpF from near-equilibrium simulations. Biochimica et Biophysica Acta (BBA) - Biomembranes 1859:8, pages 1310-1316.
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Jennie Sjöhamn, Petra Båth, Richard Neutze & Kristina Hedfalk. (2016) Applying bimolecular fluorescence complementation to screen and purify aquaporin protein:protein complexes. Protein Science 25:12, pages 2196-2208.
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Lili Yu, Roberto A. Rodriguez, L. Laurie Chen, Liao Y. Chen, George Perry, Stanton F. McHardy & Chih-Ko Yeh. (2016) 1,3-propanediol binds deep inside the channel to inhibit water permeation through aquaporins. Protein Science 25:2, pages 433-441.
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Christopher Saeui, Mohit Mathew, Lingshui Liu, Esteban Urias & Kevin Yarema. (2015) Cell Surface and Membrane Engineering: Emerging Technologies and Applications. Journal of Functional Biomaterials 6:2, pages 454-485.
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Ying Fan, Sanaz Emami, Rachel Munro, Vladimir Ladizhansky & Leonid S. Brown. 2015. Isotope Labeling of Biomolecules - Labeling Methods. Isotope Labeling of Biomolecules - Labeling Methods 193 212 .
Thomas Vogl, Gerhard G. Thallinger, Guenther Zellnig, David Drew, James M. Cregg, Anton Glieder & Maria Freigassner. (2014) Towards improved membrane protein production in Pichia pastoris: General and specific transcriptional response to membrane protein overexpression. New Biotechnology 31:6, pages 538-552.
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Anita Emmerstorfer, Tamara Wriessnegger, Melanie Hirz & Harald Pichler. (2014) Overexpression of membrane proteins from higher eukaryotes in yeasts. Applied Microbiology and Biotechnology 98:18, pages 7671-7698.
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Carlo Pirazzi, Luca Valenti, Benedetta Maria Motta, Piero Pingitore, Kristina Hedfalk, Rosellina Margherita Mancina, Maria Antonella Burza, Cesare Indiveri, Yvelise Ferro, Tiziana Montalcini, Cristina Maglio, Paola Dongiovanni, Silvia Fargion, Raffaela Rametta, Arturo Pujia, Linda Andersson, Saswati Ghosal, Malin Levin, Olov Wiklund, Michelina Iacovino, Jan Borén & Stefano Romeo. (2014) PNPLA3 has retinyl-palmitate lipase activity in human hepatic stellate cells. Human Molecular Genetics 23:15, pages 4077-4085.
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Jennie Sjöhamn & Kristina Hedfalk. (2014) Unraveling aquaporin interaction partners. Biochimica et Biophysica Acta (BBA) - General Subjects 1840:5, pages 1614-1623.
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Doryaneh Ahmadpour, Cecilia Geijer, Markus J. Tamás, Karin Lindkvist-Petersson & Stefan Hohmann. (2014) Yeast reveals unexpected roles and regulatory features of aquaporins and aquaglyceroporins. Biochimica et Biophysica Acta (BBA) - General Subjects 1840:5, pages 1482-1491.
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Piero Pingitore, Carlo Pirazzi, Rosellina M. Mancina, Benedetta M. Motta, Cesare Indiveri, Arturo Pujia, Tiziana Montalcini, Kristina Hedfalk & Stefano Romeo. (2014) Recombinant PNPLA3 protein shows triglyceride hydrolase activity and its I148M mutation results in loss of function. Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids 1841:4, pages 574-580.
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Anna Jansson, Alexandra Nafari, Kristina Hedfalk, Eva Olsson, Krister Svensson & Anke Sanz-Velasco. (2014) Monitoring the osmotic response of single yeast cells through force measurement in the environmental scanning electron microscope. Measurement Science and Technology 25:2, pages 025901.
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