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Journal of Receptors and Signal Transduction Volume 15, 1995 - Issue 1-4
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Research Article

High-Level Expression of G Protein-Coupled Receptors with the Aid of the Semliki Forest Virus Expression System

Kenneth Lundstrom Glaxo Institute for Molecular Biology, 14, chemin des Aulx, CH-1228, Plan-les-Ouates/Geneva, Switzerland
,
Ann Mills Glaxo Institute for Molecular Biology, 14, chemin des Aulx, CH-1228, Plan-les-Ouates/Geneva, Switzerland
,
Elisabeth Allet Glaxo Institute for Molecular Biology, 14, chemin des Aulx, CH-1228, Plan-les-Ouates/Geneva, Switzerland
,
Karin Ceszkowski Glaxo Institute for Molecular Biology, 14, chemin des Aulx, CH-1228, Plan-les-Ouates/Geneva, Switzerland
,
Georges Agudo Glaxo Institute for Molecular Biology, 14, chemin des Aulx, CH-1228, Plan-les-Ouates/Geneva, Switzerland
,
Andre Chollet Glaxo Institute for Molecular Biology, 14, chemin des Aulx, CH-1228, Plan-les-Ouates/Geneva, Switzerland
&
Peter Liljestrom Karolinska Institute, Novum, Center for Biotechnology, Blickagangen 6, S-141 57, Huddinge, Sweden
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Pages 23-32 | Published online: 26 Sep 2008

Citations (23)

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Read on this site (3)

Kenneth Lundstrom. (2002) SEMLIKI FOREST VIRUS-BASED EXPRESSION FOR VERSATILE USE IN RECEPTOR RESEARCH. Journal of Receptors and Signal Transduction 22:1-4, pages 229-240.
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Kenneth Lundstrom. (1999) Alphaviruses as Tools in Neurobiology and Gene Therapy. Journal of Receptors and Signal Transduction 19:1-4, pages 673-686.
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A. Scheer, K. Björ Klöf, S. Cotecchia & K. Lundstrom. (1999) Expression of the α1b-Adrenergic Receptor and G Protein Subunits in Mammalian Cell Lines Using the Semliki Forest Virus Expression System. Journal of Receptors and Signal Transduction 19:1-4, pages 369-378.
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Articles from other publishers (20)

James C. Errey & Fiona H. Marshall. 2010. GPCR Molecular Pharmacology and Drug Targeting. GPCR Molecular Pharmacology and Drug Targeting 324 384 .
Simone S. Krupka, Birgit Wiltschi, Ute Reuning, Kerstin Hölscher, Masahiko Hara & Eva-Kathrin Sinner. (2006) In vivo detection of membrane protein expression using surface plasmon enhanced fluorescence spectroscopy (SPFS). Biosensors and Bioelectronics 22:2, pages 260-267.
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Jullian Jones, Tarangsri Nivitchanyong, Christina Giblin, Valentina Ciccarone, David Judd, Stephen Gorfien, Sharon S. Krag & Michael J. Betenbaugh. (2005) Optimization of tetracycline‐responsive recombinant protein production and effect on cell growth and ER stress in mammalian cells. Biotechnology and Bioengineering 91:6, pages 722-732.
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Mattias N. E. Forsell, Yuxing Li, Maria Sundbäck, Krisha Svehla, Peter Liljeström, John R. Mascola, Richard Wyatt & Gunilla B. Karlsson Hedestam. (2005) Biochemical and Immunogenic Characterization of Soluble Human Immunodeficiency Virus Type 1 Envelope Glycoprotein Trimers Expressed by Semliki Forest Virus. Journal of Virology 79:17, pages 10902-10914.
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Stefan Raunser, Winfried Haase, Mihnea Bostina, David N. Parcej & Werner Kühlbrandt. (2005) High-yield Expression, Reconstitution and Structure of the Recombinant, Fully Functional Glutamate Transporter GLT-1 from Rattus norvegicus. Journal of Molecular Biology 351:3, pages 598-613.
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Saurabh Sen, Veli-Pekka Jaakola, Heikki Heimo, Mia Engström, Pauliina Larjomaa, Mika Scheinin, Kenneth Lundstrom & Adrian Goldman. (2003) Functional expression and direct visualization of the human α2B-adrenergic receptor and α2B-AR-green fluorescent fusion protein in mammalian cell using Semliki Forest virus vectors. Protein Expression and Purification 32:2, pages 265-275.
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Kenneth Lundstrom. (2003) Semliki Forest virus vectors for rapid and high-level expression of integral membrane proteins. Biochimica et Biophysica Acta (BBA) - Biomembranes 1610:1, pages 90-96.
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Kirstin Zimmermann, Nils Opitz, Jürgen Dedio, Christoph Renné, Werner Müller-Esterl & Stefanie Oess. (2002) NOSTRIN: A protein modulating nitric oxide release and subcellular distribution of endothelial nitric oxide synthase. Proceedings of the National Academy of Sciences 99:26, pages 17167-17172.
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Katja Björklöf, Kenneth Lundström, Liliane Abuin, Peter J. Greasley & Susanna Cotecchia. (2002) Co- and Posttranslational Modification of the α 1B -Adrenergic Receptor:  Effects on Receptor Expression and Function . Biochemistry 41:13, pages 4281-4291.
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H. D. Blasey, B. Brethon, R. Hovius, K. lundström, L. Rey, H. Vogel, A. -P. Tair & A. R. Bernard. 2002. New Developments and New Applications in Animal Cell Technology. New Developments and New Applications in Animal Cell Technology 449 455 .
Henning Cramer, Kai Schmenger, Khristina Heinrich, Angelika Horstmeyer, Hilke Böning, Andreas Breit, Albrecht Piiper, Kenneth Lundstrom, Werner Müller-Esterl & Christian Schroeder. (2001) Coupling of endothelin receptors to the ERK/MAP kinase pathway . European Journal of Biochemistry 268:20, pages 5449-5459.
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Hanns Hatt, Kerstin Lang & Günter Gisselmann. (2001) Functional Expression and Characterization of Odorant Receptors Using the Semliki Forest Virus System. Biological Chemistry 382:8.
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JÜRGEN DEDIO, PETER KÖNIG, PAULUS WOHLFART, CHRISTIAN SCHROEDER, WOLFGANG KUMMER & WERNER MÜLLER-ESTERL. (2001) NOSIP, a novel modulator of endothelial nitric oxide synthase activity. The FASEB Journal 15:1, pages 79-89.
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Donghui Ma & Min Li. (2001) Applications of display technologies to proteomic analyses. Journal of Cellular Biochemistry 84:S37, pages 34-41.
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W. Gärtner. 2000. Molecular Mechanisms in Visual Transduction. Molecular Mechanisms in Visual Transduction 297 388 .
C. Smerdou & P. Liljeström. 2000. Cell Engineering. Cell Engineering 182 210 .
Ramachandran Arudchandran, Martin J. Brown, James S Song, Stephen A Wank, Hana Haleem-Smith & Juan Rivera. (1999) Polyethylene glycol-mediated infection of non-permissive mammalian cells with semliki forest virus: application to signal transduction studies. Journal of Immunological Methods 222:1-2, pages 197-208.
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Kenneth Lundstrom, Anthony B Hawcock, Alfredo Vargas, Peter Ward, Pam Thomas & Alan Naylor. (1997) Effect of single point mutations of the human tachykinin NK1 receptor on antagonist affinity. European Journal of Pharmacology 337:1, pages 73-81.
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H Herweijer. (1997) Self-amplifying vectors for gene delivery. Advanced Drug Delivery Reviews 27:1, pages 5-16.
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Fiona H. Marshall, Kalpana Patel, Kenneth Lundstrom, Janet Camacho, Steven M. Foord & Melanie G. Lee. (1997) Characterization of [ 3 H]-prostaglandin E 2 binding to prostaglandin EP 4 receptors expressed with Semliki Forest virus . British Journal of Pharmacology 121:8, pages 1673-1678.
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