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Journal of Receptors and Signal Transduction Volume 15, 1995 - Issue 1-4
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Research Article

Expression of Human M2 Muscarinic Receptors in SF9 Cells: Characterisation and Reconstitution with G-Proteins

Freddy Heitz Marion Merrell Dow Research Institute, F-67080, Strasbourg, France
,
Steven J. McClue Marion Merrell Dow Research Institute, F-67080, Strasbourg, France
,
Bruce A. Harris Marion Merrell Dow Research Institute, F-67080, Strasbourg, France
&
Chantal Guenet Marion Merrell Dow Research Institute, F-67080, Strasbourg, France
Pages 55-70 | Published online: 26 Sep 2008

Citations (16)

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Freddy Heitz, Carole Nay & Chantal Guenet. (1997) Expression of Functional Human Muscarinic M2 Receptors in Different Insect Cell Lines. Journal of Receptors and Signal Transduction 17:1-3, pages 305-317.
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Articles from other publishers (15)

Alena Randáková, Dominik Nelic, Martina Hochmalová, Pavel Zimčík, Mutale Jane Mulenga, John Boulos & Jan Jakubík. (2021) Fusion with Promiscuous Gα16 Subunit Reveals Signaling Bias at Muscarinic Receptors. International Journal of Molecular Sciences 22:18, pages 10089.
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Rabindra V. Shivnaraine, Dennis D. Fernandes, Huiqiao Ji, Yuchong Li, Brendan Kelly, Zhenfu Zhang, Yi Rang Han, Fei Huang, Krishana S. Sankar, David N. Dubins, Jonathan V. Rocheleau, James W. Wells & Claudiu C. Gradinaru. (2016) Single-Molecule Analysis of the Supramolecular Organization of the M 2 Muscarinic Receptor and the Gα i1 Protein . Journal of the American Chemical Society 138:36, pages 11583-11598.
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Dar’ya S. Redka, Heiko Heerklotz & James W. Wells. (2013) Efficacy as an Intrinsic Property of the M 2 Muscarinic Receptor in Its Tetrameric State . Biochemistry 52:42, pages 7405-7427.
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Rabindra V. Shivnaraine, Xi-Ping Huang, Margaret Seidenberg, John Ellis & James W. Wells. (2012) Heterotropic Cooperativity within and between Protomers of an Oligomeric M 2 Muscarinic Receptor . Biochemistry 51:22, pages 4518-4540.
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Amy W.-S. Ma, John Y. Dong, Dengbo Ma & James W. Wells. (2011) Cleavage-resistant fusion proteins of the M2 muscarinic receptor and G?i1. Homotropic and heterotropic effects in the binding of ligands. Biochimica et Biophysica Acta (BBA) - General Subjects 1810:6, pages 592-602.
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Erich H. Schneider & Roland Seifert. (2010) Sf9 cells: A versatile model system to investigate the pharmacological properties of G protein-coupled receptors. Pharmacology & Therapeutics 128:3, pages 387-418.
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Hinako Suga & Tatsuya Haga. (2007) Ligand screening system using fusion proteins of G protein-coupled receptors with G protein α subunits. Neurochemistry International 51:2-4, pages 140-164.
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Paul S.-H. Park & James W. Wells. (2003) Monomers and Oligomers of the M 2 Muscarinic Cholinergic Receptor Purified from Sf 9 Cells . Biochemistry 42:44, pages 12960-12971.
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Valerie A. Mosser, Ian J. Amana & Michael I. Schimerlik. (2002) Kinetic Analysis of M2 Muscarinic Receptor Activation of Gi in Sf9 Insect Cell Membranes. Journal of Biological Chemistry 277:2, pages 922-931.
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R. T. Windh, A. J. Barr & D. R. Manning. 2000. The Pharmacology of Functional, Biochemical, and Recombinant Receptor Systems. The Pharmacology of Functional, Biochemical, and Recombinant Receptor Systems 335 362 .
Freddy Heitz, James A Holzwarth, Jean-Pierre Gies, Rebecca M Pruss, Suzanne Trumpp-Kallmeyer, Marcel F Hibert & Chantal Guenet. (1999) Site-directed mutagenesis of the putative human muscarinic M2 receptor binding site. European Journal of Pharmacology 380:2-3, pages 183-195.
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Mathieu Lachance, Nathalie Ethier, Gregor Wolbring, Paul P.M. Schnetkamp & Terence E. Hébert. (1999) Stable Association of G Proteins with β2AR Is Independent of the State of Receptor Activation. Cellular Signalling 11:7, pages 523-533.
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M. W. Beukers, C. H. W. Klaassen, W. J. De Grip, D. Verzijl, H. Timmerman & R. Leurs. (2009) Heterologous expression of rat epitope‐tagged histamine H 2 receptors in insect Sf9 cells . British Journal of Pharmacology 122:5, pages 867-874.
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Dominique Massotte, Laurence Baroche, Frédéric Simonin, Lei Yu, Brigitte Kieffer & Franc Pattus. (1997) Characterization of δ, κ, and μ Human Opioid Receptors Overexpressed in Baculovirus-infected Insect Cells. Journal of Biological Chemistry 272:32, pages 19987-19992.
Crossref
Claire Weill, François Autelitano, Chantal Guenet, Freddy Heitz, Maurice Goeldner & Brigitte Ilien. (1997) Pharmacological and structural integrity of muscarinic M2 acetylcholine receptors produced in Sf9 insect cells. European Journal of Pharmacology 333:2-3, pages 269-278.
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