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Research Article

A novel method for assessing the role of iron and its functional chelation in fibrin fibril formation: the use of scanning electron microscopy

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Pages 352-359 | Received 29 Oct 2012, Accepted 19 Dec 2012, Published online: 19 Apr 2013

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Ciska van der Schoor, Hester Magdalena Oberholzer, Megan Jean Bester & Mia-Jeanne van Rooy. (2014) The Effect of Sibutramine, a Serotonin-Norepinephrine Reuptake Inhibitor, on Platelets and Fibrin Networks of Male Sprague-Dawley Rats: A Descriptive Study. Ultrastructural Pathology 38:6, pages 399-405.
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Articles from other publishers (39)

Douglas B. Kell & Etheresia Pretorius. (2023) Are fibrinaloid microclots a cause of autoimmunity in Long Covid and other post-infection diseases?. Biochemical Journal 480:15, pages 1217-1240.
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Vance G. Nielsen, Tanner Goff, Brent D. Hunsaker & Coulter D. Neves. (2023) The Gilded Clot: Review of Metal-Modulated Platelet Activation, Coagulation, and Fibrinolysis. International Journal of Molecular Sciences 24:4, pages 3302.
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Douglas B. Kell & Etheresia Pretorius. (2022) The potential role of ischaemia–reperfusion injury in chronic, relapsing diseases such as rheumatoid arthritis, Long COVID, and ME/CFS: evidence, mechanisms, and therapeutic implications. Biochemical Journal 479:16, pages 1653-1708.
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Etheresia Pretorius, Chantelle Venter, Gert Jacobus Laubscher, Maritha J Kotze, Sunday O. Oladejo, Liam R. Watson, Kanshu Rajaratnam, Bruce W. Watson & Douglas B. Kell. (2022) Prevalence of symptoms, comorbidities, fibrin amyloid microclots and platelet pathology in individuals with Long COVID/Post-Acute Sequelae of COVID-19 (PASC). Cardiovascular Diabetology 21:1.
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Xinge Yu, Zihui Wang & Yang V. Li. (2021) Metal ion chelation enhances tissue plasminogen activator (tPA)-induced thrombolysis: an in vitro and in vivo study. Journal of Thrombosis and Thrombolysis 53:2, pages 291-301.
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Lize M. Grobbelaar, Chantelle Venter, Mare Vlok, Malebogo Ngoepe, Gert Jacobus Laubscher, Petrus Johannes Lourens, Janami Steenkamp, Douglas B. Kell & Etheresia Pretorius. (2021) SARS-CoV-2 spike protein S1 induces fibrin(ogen) resistant to fibrinolysis: implications for microclot formation in COVID-19. Bioscience Reports 41:8.
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Qinjie Liu, Jie Wu, Xufei Zhang, Xiuwen Wu, Yun Zhao & Jianan Ren. (2021) Iron homeostasis and disorders revisited in the sepsis. Free Radical Biology and Medicine 165, pages 1-13.
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Tatiana Sukhomlin. (2020) Could an acute respiratory distress syndrome in COVID‐19 infected patients be calmed down simply by iron withdrawal from lung tissues?. Journal of Medical Virology 93:2, pages 577-578.
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Marthinus Janse van Vuuren, Theodore Albertus Nell, Jonathan Ambrose Carr, Douglas B. Kell & Etheresia Pretorius. (2020) Iron Dysregulation and Inflammagens Related to Oral and Gut Health Are Central to the Development of Parkinson’s Disease. Biomolecules 11:1, pages 30.
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Etheresia Pretorius, Chantelle Venter, Gert Jacobus Laubscher, Petrus Johannes Lourens, Janami Steenkamp & Douglas B. Kell. (2020) Prevalence of readily detected amyloid blood clots in ‘unclotted’ Type 2 Diabetes Mellitus and COVID-19 plasma: a preliminary report. Cardiovascular Diabetology 19:1.
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Chantelle Venter, Johannes Andries Bezuidenhout, Gert Jacobus Laubscher, Petrus Johannes Lourens, Janami Steenkamp, Douglas B. Kell & Etheresia Pretorius. (2020) Erythrocyte, Platelet, Serum Ferritin, and P-Selectin Pathophysiology Implicated in Severe Hypercoagulation and Vascular Complications in COVID-19. International Journal of Molecular Sciences 21:21, pages 8234.
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Yali Wu, Shaonan Hu, Yunnan Ma, Bo Zhao, Wanqing Yang, Yang Lu, Pengyue Li & Shouying Du. (2020) Novel Pheretima guillelmi-derived antithrombotic protein DPf3: Identification, characterization, in vitro evaluation and antithrombotic mechanisms investigation. International Journal of Biological Macromolecules 154, pages 545-556.
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Lucas Di Meglio, Jean-Philippe DesillesVéronique OllivierMialitiana Solo NomenjanaharySara Di MeglioCatherine DeschildreStéphane LoyauJean-Marc OlivotRaphaël Blanc, Michel Piotin, Marie-Christine BoutonJean-Baptiste MichelMartine Jandrot-PerrusBenoît Ho-Tin-NoéMikael Mazighi. (2019) Acute ischemic stroke thrombi have an outer shell that impairs fibrinolysis. Neurology 93:18.
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Gloria Serena, Camron Davies, Murat Cetinbas, Ruslan I. Sadreyev & Alessio Fasano. (2019) Analysis of blood and fecal microbiome profile in patients with celiac disease. Human Microbiome Journal 11, pages 100049.
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Jun-Lin Liu, Yong-Gang Fan, Zheng-Sheng Yang, Zhan-You Wang & Chuang Guo. (2018) Iron and Alzheimer’s Disease: From Pathogenesis to Therapeutic Implications. Frontiers in Neuroscience 12.
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Jeanette N. du Plooy, Janette Bester & Etheresia Pretorius. (2018) Eryptosis in Haemochromatosis: Implications for rheology. Clinical Hemorheology and Microcirculation 69:4, pages 457-469.
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Douglas B. Kell & Etheresia Pretorius. (2018) No effects without causes: the Iron Dysregulation and Dormant Microbes hypothesis for chronic, inflammatory diseases. Biological Reviews 93:3, pages 1518-1557.
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Etheresia Pretorius, Martin J. Page, Sthembile Mbotwe & Douglas B. Kell. (2018) Lipopolysaccharide-binding protein (LBP) can reverse the amyloid state of fibrin seen or induced in Parkinson's disease. PLOS ONE 13:3, pages e0192121.
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Etheresia Pretorius, Martin J. Page, Lisa Hendricks, Nondumiso B. Nkosi, Sven R. Benson & Douglas B. Kell. (2018) Both lipopolysaccharide and lipoteichoic acids potently induce anomalous fibrin amyloid formation: assessment with novel Amytracker™ stains . Journal of The Royal Society Interface 15:139, pages 20170941.
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Bolun Du, Ruizhen Yang, Yunze He, Feng Wang & Shoudao Huang. (2017) Nondestructive inspection, testing and evaluation for Si-based, thin film and multi-junction solar cells: An overview. Renewable and Sustainable Energy Reviews 78, pages 1117-1151.
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Etheresia Pretorius, Sthembile Mbotwe & Douglas B. Kell. (2017) Lipopolysaccharide-binding protein (LBP) reverses the amyloid state of fibrin seen in plasma of type 2 diabetics with cardiovascular co-morbidities. Scientific Reports 7:1.
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Patrik Poprac, Klaudia Jomova, Miriama Simunkova, Vojtech Kollar, Christopher J. Rhodes & Marian Valko. (2017) Targeting Free Radicals in Oxidative Stress-Related Human Diseases. Trends in Pharmacological Sciences 38:7, pages 592-607.
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Douglas B. Kell & Etheresia Pretorius. (2017) Proteins behaving badly. Substoichiometric molecular control and amplification of the initiation and nature of amyloid fibril formation: lessons from and for blood clotting. Progress in Biophysics and Molecular Biology 123, pages 16-41.
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Etheresia Pretorius, Sthembile Mbotwe, Janette Bester, Christopher J. Robinson & Douglas B. Kell. (2016) Acute induction of anomalous and amyloidogenic blood clotting by molecular amplification of highly substoichiometric levels of bacterial lipopolysaccharide. Journal of The Royal Society Interface 13:122, pages 20160539.
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Etheresia Pretorius, Janette Bester & Douglas B. Kell. (2016) A Bacterial Component to Alzheimer’s-Type Dementia Seen via a Systems Biology Approach that Links Iron Dysregulation and Inflammagen Shedding to Disease. Journal of Alzheimer's Disease 53:4, pages 1237-1256.
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T. Bullock, L. J. Bruce & K. Ridgwell. (2016) Current topics in red cell biology: report on the Red Cell Special Interest Group meeting held at NHS Blood and Transplant Bristol on 30 October 2015. Transfusion Medicine 26:4, pages 241-245.
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Etheresia Pretorius, Oore-ofe O. Olumuyiwa-Akeredolu, Sthembile Mbotwe & Janette Bester. (2016) Erythrocytes and their role as health indicator: Using structure in a patient-orientated precision medicine approach. Blood Reviews 30:4, pages 263-274.
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Albe C. Swanepoel & Etheresia Pretorius. (2015) Prevention and follow-up in thromboembolic ischemic stroke: Do we need to think out of the box?. Thrombosis Research 136:6, pages 1067-1073.
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Douglas B. Kell. (2015) The transporter-mediated cellular uptake of pharmaceutical drugs is based on their metabolite-likeness and not on their bulk biophysical properties: Towards a systems pharmacology. Perspectives in Science 6, pages 66-83.
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Janette Bester, Prashilla Soma, Douglas B. Kell & Etheresia Pretorius. (2015) Viscoelastic and ultrastructural characteristics of whole blood and plasma in Alzheimer-type dementia, and the possible role of bacterial lipopolysaccharides (LPS). Oncotarget 6:34, pages 35284-35303.
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Douglas B. Kell & Etheresia Pretorius. (2015) On the translocation of bacteria and their lipopolysaccharides between blood and peripheral locations in chronic, inflammatory diseases: the central roles of LPS and LPS-induced cell death. Integrative Biology 7:11, pages 1339-1377.
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Marnie Potgieter, Janette Bester, Douglas B. Kell & Etheresia Pretorius. (2015) The dormant blood microbiome in chronic, inflammatory diseases. FEMS Microbiology Reviews 39:4, pages 567-591.
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Douglas B. Kell & Etheresia Pretorius. (2015) The simultaneous occurrence of both hypercoagulability and hypofibrinolysis in blood and serum during systemic inflammation, and the roles of iron and fibrin(ogen). Integrative Biology 7:1, pages 24-52.
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Etheresia Pretorius, Janette Bester, Natasha Vermeulen, Sajee Alummoottil, Prashilla Soma, Antoinette V Buys & Douglas B Kell. (2015) Poorly controlled type 2 diabetes is accompanied by significant morphological and ultrastructural changes in both erythrocytes and in thrombin-generated fibrin: implications for diagnostics. Cardiovascular Diabetology 14:1, pages 30.
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Vance G. Nielsen & Etheresia Pretorius. (2014) Iron-enhanced coagulation is attenuated by chelation A thrombelastographic and ultrastructural analysis. Blood Coagulation & Fibrinolysis 25:8, pages 845-850.
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Vance G. Nielsen & Etheresia Pretorius. (2014) Iron and carbon monoxide enhance coagulation and attenuate fibrinolysis by different mechanisms. Blood Coagulation & Fibrinolysis 25:7, pages 695-702.
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Etheresia Pretorius, Janette Bester, Natasha Vermeulen, Boguslaw Lipinski, George S. Gericke & Douglas B. Kell. (2014) Profound Morphological Changes in the Erythrocytes and Fibrin Networks of Patients with Hemochromatosis or with Hyperferritinemia, and Their Normalization by Iron Chelators and Other Agents. PLoS ONE 9:1, pages e85271.
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Etheresia Pretorius & Douglas B. Kell. (2014) Diagnostic morphology: biophysical indicators for iron-driven inflammatory diseases. Integr. Biol. 6:5, pages 486-510.
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Douglas B. Kell & Etheresia Pretorius. (2014) Serum ferritin is an important inflammatory disease marker, as it is mainly a leakage product from damaged cells. Metallomics 6:4, pages 748-773.
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