Advanced search
Publication Cover
Journal of Immunotoxicology Volume 6, 2009 - Issue 4: Select Reports from the “Cancer Immunotherapy and Immunomonitoring (CITIM)” Conference, May 18-21, 2009, Kiev, Ukraine
Submit an article Journal homepage
2,936
Views
46
CrossRef citations to date
0
Altmetric
KIEV MEETING: Oral Presentations

ELISPOT assays provide reproducible results among different laboratories for T-cell immune monitoring—even in hands of ELISPOT-inexperienced investigators

W. ZhangCellular Technology Ltd., Shaker Hts. Ohio, USA
,
R. CaspellCellular Technology Ltd., Shaker Hts. Ohio, USA
,
A. Y. KarulinCellular Technology Ltd., Shaker Hts. Ohio, USA
,
M. AhmadNottingham Trent University, Nottingham, UK
,
N. HaicheurHôpital Européen Georges Pompidou, Paris, France
,
A. AbdelsalamUniversity of Pittsburgh, Pittsburgh, PA, USA
,
K. JohannesenNorwegian Radium Institute, Oslo, Norway
,
V. VignardINSERM, Nantes, France
,
P. DudzikJagiellonian University Medical College, Krakow, Poland
,
K. GeorgakopoulouImmunology Center, St. Savas Cancer Hospital, Athens, Greece
,
A. MihaylovaUMBAL “Alexandrovska”, Sofia, Bulgaria
,
K. SilinaBiomedical Research Study Centre, Riga, Latvia
,
N AptsiauriHospital Universitario Virgen De la Nieves, Granada, Spain
,
V. AdamsOnyvax Ltd, London, UK
,
P. V. LehmannCorrespondence[email protected]
&
S. McArdleNottingham Trent University, Nottingham, UK
 show all
Pages 227-234 | Received 27 Jul 2009, Accepted 08 Sep 2009, Published online: 12 Nov 2009

Citations (46)

Keep up to date with the latest research on this topic with citation updates for this article.

Subscribe to citation updates

Read on this site (3)

Johannes Trück, Ruth Mitchell, Amber J Thompson, Begonia Morales-Aza, Elizabeth A Clutterbuck, Dominic F Kelly, Adam Finn & Andrew J Pollard. (2014) Effect of cryopreservation of peripheral blood mononuclear cells (PBMCs) on the variability of an antigen-specific memory B cell ELISpot. Human Vaccines & Immunotherapeutics 10:8, pages 2490-2496.
Read now
Fadi Saade, Stacey Ann Gorski & Nikolai Petrovsky. (2012) Pushing the frontiers of T-cell vaccines: accurate measurement of human T-cell responses. Expert Review of Vaccines 11:12, pages 1459-1470.
Read now
Meredith Slota, Jong-Baeck Lim, Yushe Dang & Mary L Disis. (2011) ELISpot for measuring human immune responses to vaccines. Expert Review of Vaccines 10:3, pages 299-306.
Read now

Articles from other publishers (43)

Gwenn Waerlop, Geert Leroux-Roels, Anke Pagnon, Sarah Begue, Bruno Salaun, Michel Janssens, Donata Medaglini, Elena Pettini, Emanuele Montomoli, Elena Gianchecchi, Teresa Lambe, Leila Godfrey, Maireid Bull, Duncan Bellamy, Håkon Amdam, Geir Bredholt, Rebecca Jane Cox & Frédéric Clement. (2023) Proficiency tests to evaluate the impact on assay outcomes of harmonized influenza-specific Intracellular Cytokine Staining (ICS) and IFN-ɣ Enzyme-Linked ImmunoSpot (ELISpot) protocols. Journal of Immunological Methods 523, pages 113584.
Crossref
Jaclyn C. Law & Tania H. Watts. (2023) Considerations for Choosing T Cell Assays during a Pandemic. The Journal of Immunology 211:2, pages 169-174.
Crossref
Alexey Sereda, Anna Kazakova, Sanzhi Namsrayn, Mikhail Vlasov, Irina Sindryakova & Denis Kolbasov. (2023) Subsequent Immunization of Pigs with African Swine Fever Virus (ASFV) Seroimmunotype IV Vaccine Strain FK-32/135 and by Recombinant Plasmid DNA Containing the CD2v Derived from MK-200 ASFV Seroimmunotype III Strain Does Not Protect from Challenge with ASFV Seroimmunotype III. Vaccines 11:5, pages 1007.
Crossref
Paul V. Lehmann, Diana R. Roen & Alexander A. Lehmann. 2023. Computational Vaccine Design. Computational Vaccine Design 69 88 .
S S VYAVAHARE, M K JHALA & B B BHANDERI. (2022) Comparative analysis of intradermal tuberculin test and γ-interferon assay for diagnosis of bovine tuberculosis. The Indian Journal of Animal Sciences 92:9.
Crossref
Tong-yuan Yang, Manuela Braun, Wibke Lembke, Fraser McBlane, John Kamerud, Stephen DeWall, Edit Tarcsa, Xiaodong Fang, Lena Hofer, Uma Kavita, Vijay V. Upreti, Swati Gupta, LiNa Loo, Alison J. Johnson, Rakesh Kantilal Chandode, Kay-Gunnar Stubenrauch, Maya Vinzing, Cindy Q. Xia & Vibha Jawa. (2022) Immunogenicity assessment of AAV-based gene therapies: An IQ consortium industry white paper. Molecular Therapy - Methods & Clinical Development 26, pages 471-494.
Crossref
Yorick Janssens, Jasper Joye, Gwenn Waerlop, Frédéric Clement, Geert Leroux-Roels & Isabel Leroux-Roels. (2022) The role of cell-mediated immunity against influenza and its implications for vaccine evaluation. Frontiers in Immunology 13.
Crossref
Anneliese S. Ashhurst, Cameron C. Hanna, Richard J. Payne & Warwick J. Britton. 2022. Bacterial Vaccines. Bacterial Vaccines 301 323 .
Chenguang Wang, Zhixin Wang, Gary L. Rosner, Warner K. Huh, Richard B.S. Roden & Sejong Bae. (2020) A batch‐effect adjusted Simon's two‐stage design for cancer vaccine clinical studies. Biometrics 77:3, pages 1075-1088.
Crossref
Elena Ranieri, Giuseppe Stefano Netti & Margherita Gigante. 2021. Cytotoxic T-Cells. Cytotoxic T-Cells 65 77 .
Almaz R Imatdinov, Anna S Kazakova, Milanko Šekler, Diana Yu Morozova, Valentina M Lyska, Ilya A Titov, Timofey Sevskikh & Alexey D Sereda. (2020) Immunization of Pigs with Recombinant Plasmids Containing Genes of Ubiquitinated p30, p54 and CD2v Proteins of African Swine Fever Virus. Acta Veterinaria 70:1, pages 92-109.
Crossref
Christopher R. Wilcox & Christine E. Jones. (2018) Beyond Passive Immunity: Is There Priming of the Fetal Immune System Following Vaccination in Pregnancy and What Are the Potential Clinical Implications?. Frontiers in Immunology 9.
Crossref
Alexey Y. Karulin, Zoltán Megyesi, Richard Caspell, Jodi Hanson & Paul V. Lehmann. 2018. Handbook of ELISPOT. Handbook of ELISPOT 95 113 .
Zoltán Megyesi, Paul V. Lehmann & Alexey Y. Karulin. 2018. Handbook of ELISPOT. Handbook of ELISPOT 115 131 .
Annemarie Schiller, Ting Zhang, Ruliang Li, Andrea Duechting, Srividya Sundararaman, Anna Przybyla, Stefanie Kuerten & Paul Lehmann. (2017) A Positive Control for Detection of Functional CD4 T Cells in PBMC: The CPI Pool. Cells 6:4, pages 47.
Crossref
José Manuel Rojas, Miguel Avia, Elena Pascual, Noemí Sevilla & Verónica Martín. (2017) Vaccination with recombinant adenovirus expressing peste des petits ruminants virus-F or -H proteins elicits T cell responses to epitopes that arises during PPRV infection. Veterinary Research 48:1.
Crossref
Eberhard Wieland. 2016. Personalized Immunosuppression in Transplantation. Personalized Immunosuppression in Transplantation 227 257 .
Niels V. Rekers, Matthias G. von Herrath & Johnna D. Wesley. (2015) Immunotherapies and immune biomarkers in Type 1 diabetes: A partnership for success. Clinical Immunology 161:1, pages 37-43.
Crossref
Marie Wunsch, Wenji Zhang, Jodi Hanson, Richard Caspell, Alexey Karulin, Mascha Recks, Stefanie Kuerten, Srividya Sundararaman & Paul Lehmann. (2015) Characterization of the HCMV-Specific CD4 T Cell Responses that Are Associated with Protective Immunity. Viruses 7:8, pages 4414-4437.
Crossref
Lynda Coughlan & Teresa Lambe. (2015) Measuring Cellular Immunity to Influenza: Methods of Detection, Applications and Challenges. Vaccines 3:2, pages 293-319.
Crossref
Jodi Hanson, Srividya Sundararaman, Richard Caspell, Edith Karacsony, Alexey Karulin & Paul Lehmann. (2015) ELISPOT Assays in 384-Well Format: Up to 30 Data Points with One Million Cells. Cells 4:1, pages 71-83.
Crossref
Alexey Karulin, Kinga Karacsony, Wenji Zhang, Oleg Targoni, Ioana Moldovan, Marcus Dittrich, Srividya Sundararaman & Paul Lehmann. (2015) ELISPOTs Produced by CD8 and CD4 Cells Follow Log Normal Size Distribution Permitting Objective Counting. Cells 4:1, pages 56-70.
Crossref
Marie Wunsch, Richard Caspell, Stefanie Kuerten, Paul Lehmann & Srividya Sundararaman. (2015) Serial Measurements of Apoptotic Cell Numbers Provide Better Acceptance Criterion for PBMC Quality than a Single Measurement Prior to the T Cell Assay. Cells 4:1, pages 40-55.
Crossref
Srividya Sundararaman, Alexey Karulin, Tameem Ansari, Nadine BenHamouda, Judith Gottwein, Sreenivas Laxmanan, Steven Levine, John Loffredo, Stephanie McArdle, Christine Neudoerfl, Diana Roen, Karina Silina, Mackenzie Welch & Paul Lehmann. (2015) High Reproducibility of ELISPOT Counts from Nine Different Laboratories. Cells 4:1, pages 21-39.
Crossref
Lisa H. Butterfield & Mary Jo Buffo. 2014. Molecular Diagnostics for Melanoma. Molecular Diagnostics for Melanoma 71 82 .
Elena Ranieri, Iulia Popescu & Margherita Gigante. 2014. Cytotoxic T-Cells. Cytotoxic T-Cells 75 86 .
Chenggang Jin, Diana Roen, Paul Lehmann & Gottfried Kellermann. (2013) An Enhanced ELISPOT Assay for Sensitive Detection of Antigen-Specific T Cell Responses to Borrelia burgdorferi. Cells 2:3, pages 607-620.
Crossref
O. Bestard, E. Crespo, M. Stein, M. Lúcia, D.L. Roelen, Y.J. de Vaal, M.P. Hernandez-Fuentes, L. Chatenoud, K.J. Wood, F.H. Claas, J.M. Cruzado, J.M. Grinyó, H.D. Volk & P. Reinke. (2013) Cross-Validation of IFN-γ Elispot Assay for Measuring Alloreactive Memory/Effector T Cell Responses in Renal Transplant Recipients. American Journal of Transplantation 13:7, pages 1880-1890.
Crossref
O. Bestard, M. Lucia, E. Crespo, B. Van Liempt, D. Palacio, E. Melilli, J. Torras, I. Llaudó, G. Cerezo, O. Taco, S. Gil-Vernet, J.M. Grinyó & J.M. Cruzado. (2013) Pretransplant Immediately Early-1-Specific T Cell Responses Provide Protection for CMV Infection After Kidney Transplantation. American Journal of Transplantation 13:7, pages 1793-1805.
Crossref
Hosun Park. (2013) Evaluation Methods for the Immunogenicity of Varicella and Zoster Vaccines. Journal of Bacteriology and Virology 43:4, pages 253.
Crossref
Sandra A. Calarota & Fausto Baldanti. (2013) Enumeration and Characterization of Human Memory T Cells by Enzyme-Linked Immunospot Assays. Clinical and Developmental Immunology 2013, pages 1-8.
Crossref
Paul V. Lehmann & Srividya Sundararaman. 2013. The Tumor Immunoenvironment. The Tumor Immunoenvironment 725 740 .
C. Costa, A. Saldan, F. Sinesi, F. Sidoti, C. Balloco, S. Simeone, A. Piceghello, S. Mantovani, A. Di Nauta, P. Solidoro & R. Cavallo. (2012) The Lack and Cytomegalovirus-Specific Cellular Immune Response May Contribute to the Onset of Organ Infection and Disease in Lung Transplant Recipients. International Journal of Immunopathology and Pharmacology 25:4, pages 1003-1009.
Crossref
Hari Ramachandran, Jessica Laux, Ioana Moldovan, Richard Caspell, Paul V. Lehmann & Ramu A. Subbramanian. (2012) Optimal Thawing of Cryopreserved Peripheral Blood Mononuclear Cells for Use in High-Throughput Human Immune Monitoring Studies. Cells 1:3, pages 313-324.
Crossref
Camilla Tincati, Amedeo J. Cappione III & Jennifer E. Snyder-Cappione. (2012) Distinguishing Latent from Active Mycobacterium tuberculosis Infection Using Elispot Assays: Looking Beyond Interferon-gamma. Cells 1:2, pages 89-99.
Crossref
Michael Oellerich, Gunnar Brandhorst, Maria Shipkova & Eberhard Wieland. 2012. Therapeutic Drug Monitoring. Therapeutic Drug Monitoring 349 372 .
Marcus Dittrich & Paul V. Lehmann. 2012. Handbook of ELISPOT. Handbook of ELISPOT 173 183 .
Wenji Zhang & Paul V. Lehmann. 2012. Handbook of ELISPOT. Handbook of ELISPOT 155 171 .
Paul V. Lehmann & Wenji Zhang. 2012. Handbook of ELISPOT. Handbook of ELISPOT 3 23 .
Lisa H. Butterfield, A. Karolina Palucka, Cedrik M. Britten, Madhav V. Dhodapkar, Leif Håkansson, Sylvia Janetzki, Yutaka Kawakami, Thomas-Oliver Kleen, Peter P. Lee, Cristina Maccalli, Holden T. Maecker, Vernon C. Maino, Michele Maio, Anatoli Malyguine, Giuseppe Masucci, Graham Pawelec, Douglas M. Potter, Licia Rivoltini, Lupe G. Salazar, Dolores J. Schendel, Craig L. SlingluffJrJr, Wenru Song, David F. Stroncek, Hideaki Tahara, Magdalena Thurin, Giorgio Trinchieri, Sjoerd H. van Der Burg, Theresa L. Whiteside, Jon M. Wigginton, Francesco Marincola, Samir Khleif, Bernard A. Fox & Mary L. Disis. (2011) Recommendations from the iSBTc-SITC/FDA/NCI Workshop on Immunotherapy Biomarkers. Clinical Cancer Research 17:10, pages 3064-3076.
Crossref
C. Costa, S. Astegiano, M.E. Terlizzi, F. Sidoti, A. Curtoni, P. Solidoro, S. Baldi, M. Bergallo & R. Cavallo. (2011) Evaluation and Significance of Cytomegalovirus-Specific Cellular Immune Response in Lung Transplant Recipients. Transplantation Proceedings 43:4, pages 1159-1161.
Crossref
Jeffery J. Auletta, Elizabeth A. Zale, Jean F. Welter & Luis A. Solchaga. (2011) Fibroblast Growth Factor-2 Enhances Expansion of Human Bone Marrow-Derived Mesenchymal Stromal Cells without Diminishing Their Immunosuppressive Potential. Stem Cells International 2011, pages 1-10.
Crossref
A. Mander, C. Gouttefangeas, C. Ottensmeier, M. J. P. Welters, L. Low, S. H. van der Burg & C. M. Britten. (2010) Serum is not required for ex vivo IFN-γ ELISPOT: a collaborative study of different protocols from the European CIMT Immunoguiding Program. Cancer Immunology, Immunotherapy 59:4, pages 619-627.
Crossref

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Order Reprints Request Corporate Permissions

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

Request Academic Permissions

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.

Related research

People also read lists articles that other readers of this article have read.

Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.

Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.