Environmental influences on for-mediated oviposition decisions in Drosophila melanogaster

Abstract

Deciding whether or not to lay an egg on a given substrate is an important task undertaken by females of many arthropods. It involves perceiving the environment (e.g. quality of the substrate, temperature, and humidity), formulating a decision, and then conducting the appropriate behaviours to oviposit. This oviposition site selection (OSS) provides a useful system for studying simple decision-making. OSS in fruit flies, Drosophila melanogaster, is influenced by both genetic and environmental variation. Naturally occurring allelic variation in the foraging gene (for) is known to affect OSS. Given a choice of high- and low-nutrient oviposition substrates, groups of rovers (for^R) are known to lay significantly more of their eggs on low-nutrient sites than sitters (for^s) and sitter mutants (for^s2). Here we ask three questions: (1) Is the role of for in OSS affected by the availability of alternate oviposition sites? (2) Is the role of for in OSS sensitive to the density of ovipositing females? and (3) Does the gustatory sensation of yeast play a role in for-mediated variation in OSS? We find a role of choice and female density in rover/sitter differences in OSS, as well as a role of for in response to glycerol, an indicator of yeast. The role of for in OSS decision-making is complex and multi-faceted and should prove fertile ground for further research into the factors affecting decision-making behaviours.

Keywords:

• Oviposition
• decision-making
• Drosophila
• glycerol
• proboscis extension
• foraging gene
• social cues

Acknowledgements

We dedicate this paper to Marla Sokolowski, an incredible mentor, and an inspiring scientist. We thank M.B. Sokolowski, A.C. Mason, M.R. Terebiznik, A.H. Edelsparre, and M.W. McConnell for constructive input and criticism.

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

This research was supported by a Natural Sciences and Engineering Research Council (NSERC) Discovery operating grant to M.J.F.