Experımental Study: The Effect Of 20% Intralıpıd Emulsıon Therapy In Cerebral Ischemıc Reperfusıon

ABSTRACT

Background

This experimental study was conducted to investigate the effect of 20% Intralipid Emulsion (ILE) treatment on Cerebral Ischemia Reperfusion Injury (CIRI) after reperfusion in acute ischemic stroke.

Methods

In this experimental study, seven rats without any intervention (control group), seven rats (sham group) for which CIRI was created after the common carotid artery was ligated for 2 hours, and seven rats who were treated with 20% ILE after CIRI (CIRI + ILE group) were sacrificed after 24 hours, and histopathological findings were investigated.

Results

In rats that were not treated after CIRI, 52.7% had level-1, 32.7% had level-2. and 14.5% had level-3. histopathological findings. While 72.2% of the rats treated with ILE had level-1 and 27.8% had level-2 findings, no level-3 histopathological findings were detected in any of the rats. While no signs of coagulative necrosis, spongiosis of surrounding tissue and polymorphonuclear leukocytes were observed histopathological in any of the rats given ILE, there was no macrophages finding in 85.6% of the rats. ILE treatment also reduced the histopathological findings of eosinophilic neurons, astrogliosis, neovascularization, vascular thrombosis and mononuclear inflammatory cells.

Conclusion

This study showed that 20% ILE treatment reduces the histopathological damage seen in cerebral ischemia and CIRI.

KEYWORDS:

• Experimental study
• stroke
• cerebral histopathological findings
• cerebral reperfusion injury
• Intralipid Emulsion therapy

Acknowledgments

We thank Mrs.Emire for statistical support.

Disclosure statement

There is no conflict of interest between the authors in this study.

Financial support

No financial support has been received for this study. The authors were self-funded for this study.

Ethics statement

All authors declared that they comply with the Research and Publication Ethics rules. This experimental study was conducted after obtaining approval from Yeditepe University Animal Research local ethics committee (number: 631; date of decision: 22.12.2017).

Study concept and design

Study plan and design: DNÖ, CŞ, ES, EH; data collection: CŞ, SS, DTŞ, ES, EH; analysis and interpretation of data: DNÖ, CŞ, DŞ; drafting: DNÖ, CŞ; operational control: DNÖ; critical revision: DNÖ. All authors have read and approved the final article.

Additional information

Funding

No funding support was received for this study. The authors covered the study expenses themselves.