Platelet-rich plasma improves impaired glucose hemostasis, disrupted insulin secretion, and pancreatic oxidative stress in streptozotocin-induced diabetic rat

Abstract

Our study aimed to investigate the effects of platelet-rich plasma (PRP) on impaired glucose homeostasis, disrupted islet insulin secretion, and pancreatic oxidative status in streptozotocin (STZ)-diabetic rats. A total of 64 Sprague-Dawley male were randomized to four groups including controls, diabetes, control-PRP, and diabetes-PRP. The rats received the PRP (0.5 ml/kg, SC injection) twice weekly for 4 weeks. Plasma glucose and insulin levels, pancreatic oxidative stress markers and islet insulin secretion and content were measured. Compared with the control group, in the diabetic group, increased plasma glucose and malondialdehyde (MDA) levels and decreased plasma insulin level, islet insulin secretion, pancreatic superoxide dismutase (SOD), and catalase activities were observed. PRP treatment significantly reduced plasma glucose and MDA levels and enhanced plasma insulin, antioxidant enzyme activity, islet insulin secretion, and content in the diabetic rats. These findings showed that PRP can improve pancreatic islet insulin secretion, pancreatic oxidative stress and regulate plasma insulin and glucose levels in diabetic rats.

Keywords:

• PRP
• diabetic rat
• islet insulin secretion
• oxidative stress

Acknowledgments

This article is a part of the M.Sc. Thesis of Marzieh Zarin. The authors wish to thank Dr. Nasrin Shokrpour at the Research Consultation Centre (RCC) for editing this manuscript.

Author contributions

Marziyeh Zarin: Investigation, Visualization, Data analysis.

Narges Karbalaei: Conceptualization, Supervision, Project Administration, review & editing.

Sara Keshtgar: Conceptualization, Validation, review & editing.

Marzieh Nemati: Data analysis, Writing—original draft.

Disclosure statement

The authors declare no conflict of interest.

Additional information

Funding

This work was financially supported by Shiraz University of Medical Sciences, Shiraz, Iran (grant no. 16720).