The RANKL rs12585014 polymorphism is associated with age at menarche in postmenopausal women with hip fracture

Abstract

The RANK/RANKL/OPG signaling is important in the regulation of bone turnover. The aim of the present work was to analyze the rs3018362 and rs12585014 polymorphisms in the RANK and RANKL genes, as well as risk factors in postmenopausal women. Women with hip fracture, with femoral neck osteoporosis and controls (n = 646) were recruited. From these, 303 women who fulfill the inclusion criteria were genotyped using real-time PCR with TaqMan probes. There were no associations of the rs3018362 and rs12585014 with osteoporosis or fracture. When women were divided by age at menarche, the rs12585014 GG genotype was strongly associated with age at menarche >13 years [p = .00774, OR = 6.429 (1.907–21.103)] in women with hip fracture. Significant differences in risk factors such as body mass index, age at menopause, use of estrogens, the presence of hypertension, and diabetes mellitus were found. Carrying the GG genotype of rs12585014 entails a higher risk of having menarche later (>13 years), which could involves a greater risk of fractures. The rs3018362 and rs12585014 do not seem to be associated with hip osteoporosis or hip fracture in Mexican women.

摘要

RANK/RANKL/OPG 信号通路在骨转换的调控中非常重要。本研究的目的是分析rs3018362 和 rs12585014的多态性和危险因素。本研究纳入髋部骨折的女性646人, 包括股骨颈骨质疏松组和对照组。303例符合纳入标准的病例使用 TaqMan探针实时PCR分析基因型。 rs3018362 和 rs12585014与骨质疏松或骨折无关。按初潮年龄分组后, 发现rs12585014与绝经后髋部骨折患者的初潮年龄>13岁相关性明显[p=.00774, OR=6.429 (1.907–21.103)] 。体质数、绝经年龄、应用雌激素、合并高血压、糖尿病也是影响因素。携带 rs12585014基因导致初潮年龄>13岁, 骨折的风险会增加。在墨西哥女性中 rs3018362 和 rs12585014似乎与髋部骨质疏松或骨折无关。

Keywords:

• Polymorphisms
• osteoporosis
• fracture
• RANK
• RANKL

Disclosure statement

The authors report no conflicts of interest.

Additional information

Funding

This work was supported by Consejo Nacional de Ciencia y Tecnología under Grant SALUD-2007-C01–69706.