Live birth following a single oocyte fertilized with ICSI and embryo biopsy at blastocyst stage: a case report

Abstract

In this report, we present a case of a couple who obtained a live birth with a single oocyte fertilized by ICSI. Two oocytes were collected at 35.5 hours (h) post trigger and both were at metaphase II when sperm injection was performed (38 h). At 18 h post injection, one oocyte was fertilized, developed to the blastocyst stage and underwent to trophectoderm biopsy for preimplantation genetic testing on day 5. Following biopsy, the blastocyst was vitrified and 4 h after warming procedure, the euploid blastocyst was replaced to the uterine cavity. Healthy live birth was obtained after 39 weeks of gestation.

摘要

在这篇个案报道中, 我们报道了一对夫妇经过ICSI后获得了单卵受精的胚胎后得到活产的病例。在扳机后35.5小时获得了2个卵母细胞, 进行精子注射时（38小时）两个卵母细胞均处于M II期。注射后18小时, 一个卵母细胞受精, 发育到囊胚期, 为了进行胚胎植入前的基因诊断, 在囊胚第5天进行滋养细胞外胚层的活检。活检后的囊胚进行玻璃化冷冻, 4小时后复苏, 然后将整倍体囊胚移至宫腔。妊娠39周胚胎健康分娩。

Keywords:

• In vitro fertilization (IVF)
• intracytoplasmic sperm injection (ICSI)
• fertilization and embryo culture
• blastocyst biopsy
• preimplantation genetic testing
• live birth

Disclosure statement

No potential conflict of interest was reported by the author(s).