Zinc, copper, sirtuin 1 concentration, and glucose metabolism parameters in the blood of women with polycystic ovary syndrome

Abstract

The aim of this study was to assess concentration of zinc (Zn), copper (Cu), and sirtuin 1 (SIRT1) in serum of women with polycystic ovary syndrome (PCOS) and their relationships with glucose metabolism parameters. The study included 76 women with PCOS aged between 17 and 39 years old. The blood was collected according to the routine procedure during the follicular phase. Zn and Cu concentrations were performed by flame atomic absorption spectrometry (FAAS). Glucose concentration was estimated by colorimetric methods. Insulin (INs) concentration was determined by enzyme-linked immunosorbent assay. SIRT1 concentration was determined using commercial test. We found higher Cu concentration and Cu/Zn value in the serum of women with PCOS with overweight/obesity. Early stage of insulin resistance (IR) in the group of women with PCOS affected on higher Cu concentration and Cu/Zn value. However, increased value of body mass index, waist-hip ratio and homeostatic model assessment of insulin resistance (HOMA-IR) was not associated with changes in Zn and SIRT1 concentration. Further disturbances in glucose metabolic parameters in the blood of women with PCOS were intensified by overweight/obesity and IR. Overweight/obesity and IR in the women with PCOS increase disorders in Cu homeostasis and glucose metabolism parameters.

摘要：

本研究旨在探讨多囊卵巢综合征（PCOS）患者血清锌（Zn）、铜（Cu）和sirtuin1（SIRT1）的浓度及其与糖代谢参数的关系。这项研究包括76名多囊卵巢综合征女性, 年龄在17至39岁之间。分别在卵泡期按常规程序采血。用火焰原子吸收光谱法（FAAS）测定锌和铜的浓度。用比色法测定葡萄糖浓度。采用酶联免疫吸附法测定胰岛素（INs）浓度。采用工业试验测定SIRT1浓度。我们发现超重/肥胖的多囊卵巢综合征女性血清中铜浓度和铜/锌比值较高。多囊卵巢综合征患者早期胰岛素抵抗(IR)与较高的铜浓度和铜/锌比值有关。然而, 体重指数、腰臀比和胰岛素抵抗的稳态模型评估值（HOMA-IR）的增加与锌和SIRT1浓度的变化无关。超重/肥胖和胰岛素抵抗使多囊卵巢综合征患者葡萄糖代谢参数进一步紊乱, 同时使铜稳态和葡萄糖代谢参数更加紊乱。

Keywords:

• Polycystic ovary syndrome
• insulin resistance
• body mass index

Disclosure statement

The authors declare no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.

Additional information

Funding

This research was supported by the ST.D170.18.002, Wroclaw Medical University and Uniwersytet Medyczny im. Piastów Slaskich we Wroclawiu.