The role of follicular anti-Mullerian hormone in women undergoing IVF/ICSI with regard to size, oocyte presence, sociodemographic parameters, treatment variables, and other hormones and vitamins

Abstract

Aims

The aim of this study was to evaluate the relationship between follicular anti-Mullerian hormone (fAMH) regarding follicle size, the presence of an oocyte, sociodemographic parameters, and other hormones and vitamins in follicular fluid.

Materials and methods

This prospective exploratory cohort study included 376 follicles from 61 women undergoing IVF/ICSI cycles. The size of each follicle was measured individually, and they were divided into a large and a small group according to their size. The presence of oocytes was detected on the day of oocyte retrieval. Sociodemographic factors were documented during the course of preliminary testing. Then, other parameters of patients’ serum and follicular fluid were measured.

Results

Small follicles contained a significantly higher concentration of fAMH than large ones did. We showed that the presence of an oocyte in a follicle is associated with a significantly higher fAMH level than in those without one (p < .001). There exists a significant but weak correlation between fAMH and the sociodemographic parameter of patients’ age (r = −0.11, p < .001). We did not find a correlation with the patients’ BMI (r = 0.03, p < .006). We also investigated the connections between fAMH and other parameters, such as vitamin D (r = −0.13, p < .001), LH (r = 0.35, p < .001)), and progesterone (r = −0.21, p < .001) in follicular fluid.

Conclusions

This knowledge can be useful for the future development of reproductive medicine. Our results can provide an important building block for this matter.

卵泡抗苗勒管激素在接受IVF/ICSI的女性中与大小、卵母细胞存在、社会人口学参数、治疗变量以及其他激素和维生素的作用 摘要

目的：本研究的目的是评估卵泡抗苗勒氏激素（fAMH）与卵泡大小、卵母细胞的存在、社会人口学参数以及卵泡液中其他激素和维生素之间的关系。

材料和方法：这项前瞻性探索性队列研究包括来自61名接受IVF/ICSI周期的女性的376个卵泡。分别测量每个卵泡的大小, 并根据大小将其分为大卵泡组和小卵泡组。取卵当天检测到卵母细胞的存在。在初步评估过程中记录了社会人口学因素, 然后测定患者血清和卵泡液的其他参数。

结果：小卵泡的fAMH水平显著高于大卵泡。我们发现, 与没有卵母细胞的卵泡相比, 卵泡中存在卵母细胞与显著更高的fAMH水平相关（p<0.001）。fAMH与患者年龄的社会人口学参数之间存在显著但较弱的相关性（r=-0.11, p<0.001）。我们没有发现与患者BMI的相关性（r=0.03, p<0.006）。我们还研究了fAMH与卵泡液中维生素D（r=-0.13, p<0.001）、LH（r=0.35, p<0.001）和孕酮（r= -0.21, p<0.001）之间的关系。

结论：这些知识可用于生殖医学的未来发展。我们的结果可为这个问题提供一个重要的基石。

Keywords:

• Anti-Mullerian hormone
• IVF
• ICSI
• follicular fluid

Acknowledgments

We thank Dr. Brandon Greene for his assistance with the statistical analysis of this study.

Disclosure statement

The authors report no conflicts of interest. Parts of this work were published in advance as posters at the DGGG (Deutsche Gesellschaft für Gynäkologie und Geburtshilfe) and ESHRE (European Society of Human Reproduction and Embryology) congresses in 2020. Samples used in this study were stored in Marburg Biobank CBBMR.