Fast acetone tissue processing of human organs provides tissue characteristics equal to conventional processing

ABSTRACT

Routine preparation of paraffin embedded tissue for histopathological diagnosis, here termed conventional histological technique (CT), whether performed manually or using an automated system, requires approximately 12 h. We developed earlier a rapid acetone dehydration technique (AT) for processing biopsies of nervous tissue that meets requirements for preserving tissue morphology and staining properties, and reduces processing time to 3.3 h. We compared the morphology and staining properties of human organ biopsies including adrenal gland, liver, ovary, pancreas, prostate, testis and thyroid prepared using both AT and CT. Following fixation with 10% formaldehyde and processing by either AT or CT, sections were stained using routine and special staining, and immunohistochemical methods. We evaluated nuclear and cytoplasmic staining, staining intensity, sharpness of images and presence of artifacts such as cracking and folding. AT preserved the morphology and staining properties of the tissues as well as CT. Consequently, the rapid AT procedure is a promising alternative technique for tissue processing.

KEYWORDS:

• Acetone
• conventional processing
• histochemistry
• human
• immunohistochemistry
• organs
• tissue processing

Acknowledgments

We thank Biol. René Reynaga-Piña and QFB Adriana I. Gallardo-Gómez for technical assistance with processing and immunostaining the samples and Stephanie G. for reviewing the English manuscript. R. García-Garza and D. Salas-Treviño were equal contributors to this work.

Disclosure statement

The authors declare no conflict of interest.