Usefulness of serum opsonic activity measured by chemiluminescence method to assess the invasiveness of colorectal endoscopic mucosal dissection

Abstract

Endoscopic sub-mucosal dissection (ESD) is considered as a low-invasive treatment for early-stage colorectal cancer, but the degree of invasiveness has not been well investigated. The aim of this study was to evaluate the physical stress due to colorectal ESD based on changes in serum opsonic activity (SOA). SOA was examined by measuring reactive oxygen species (ROS) produced by neutrophils using lucigenin-dependent chemiluminescence (LgCL) and luminol-dependent chemiluminescence (LmCL). Sixty-nine patients were enrolled into the study and examined SOA in the morning of the day of ESD, the next day, and at four days after ESD. The peak height (PH) and area under the curve (AUC) of LgCL showed no significant difference between the day and the next day, whereas the PH and AUC for LgCL were significantly higher four days after ESD than on the day of ESD (p < .05). In contrast, the PH and AUC of LmCL showed no significant changes during the ESD perioperative period. This difference suggests that SOA changes during the colorectal ESD perioperative period involved minor increases in the production of lower-toxicity ROS. This finding supports the position that ESD is a technique that does not generate a great deal of physical stress. On the other hand, a significant increase in SOA at four days after colorectal ESD suggests that care is needed with postoperative management even after the patient has started to eat meals again.

Keywords:

• Chemiluminescence method
• colorectal endoscopic sub-mucosal dissection
• early colorectal cancer
• serum opsonic activity

Author contributions

D. C., T. S. and T. A. wrote the manuscript and interpreted the data. D. C., T. A., K. S., K. A., K. M., N. A., S. S., and S. H. performed data management and analysis. D. C. and T. S. conceived the study design. S. K., K. Y., T. T., H. K., H. H., M. S., H. S., T. M., S. N., and S. F. played a role in reviewing and revising the manuscript. All authors approved the final draft of the manuscript.

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This study was supported in part by Interdisciplinary Collaborative Research Grant for Young Scientists, Hirosaki University.