Abstract
Aerobic organisms including the gut microbiota have an essential antioxidant status, as a result of which these bacteria protect organisms from various pathologies and diseases. The goal of the given investigation is (1) the isolation and purification of the isoforms of endogenous О2−-producing associate from gastrointestinal bacteria (Lactobacillus rhamnosus, Lactobacillus acidophilus, Bifidobacterium bifidum); (2) determination of the effective concentrations of exogenous О2− produced by a complex of NADPH-containing protein component and Fe(III) (NPC-Fe(III)) from raspberries on the growth of the gastrointestinal bacteria in a nutrient medium in vitro. Ion-exchange chromatography on cellulose DE-52 and gel filtration on Sephadex G-100 at the pH of 9.5 was used to isolate and purify the NLP-Nox isoforms. Specific maximal optical absorption spectra of the Nox isoforms were observed in a weakly opalescent aqueous solution of the NLP-Nox isoforms. The specific contents of these NLP-Nox isoforms, as well as their composition, the stationary concentration of produced О2−, and the mechanism of О2− production were determined. The stimulating effect on the growth of these gastrointestinal bacteria in the nutrient medium of MRS broth and MRS agar in vitro under the influence of О2−, as a product of a new thermostable and acid-stable complex NPC-Fe(III) was determined. The NPC-Fe(III) complex, from raspberries was determined as well. Thus, for the first time, the isolation and purification of О2−- producing thermostable NADPH-containing lipoprotein–NADPH oxidase (NLP-Nox) associate from gastrointestinal bacteria membranes (continuously producing О2− under the aerobic conditions), and the stimulation of these bacteria growth by О2− formed by the complex from raspberries were demonstrated.
KEY POLICY HIGHLIGHTS
The О2−-producing associate NLP-Nox was isolated and purified from the gut microbiota.
NLP-Nox associate produces О2− by using a protein-bound non-free NADPH as a substrate.
The NPC-Fe(III) isolated from raspberries generates О2−.
The effective quantities of О2− promotes the growth and development of bacteria.
Authors contributions
Study Concept and Design: R.S., S.F., R.M., G.S., H.S., M.B., M.M., M.S., A.M. Acquisition of Data: R.S., S.F., R.M., G.S., H.S., M.B., H.Y., M.M., M.S., A.M. Analysis and Interpretation of the Data: R.S., S.F., R.M., G.S., H.S., M.B., H.Y., M.M., M.S., A.M. Drafting of the Manuscript: R.S., A.M. Critical Revision of the Manuscript for Important Intellectual Content: R.S., S.F., R.M., G.S., H.S., M.B., M.M., M.S., A.M. All authors have read and agreed to the published version of the manuscript.
Disclosure statement
No potential conflict of interest was reported by the author(s).
Data availability statement
Data can be made available by the corresponding author upon reasonable request.
Correction Statement
This article has been corrected with minor changes. These changes do not impact the academic content of the article.