Oridonin loaded peptide nanovesicles alleviate nonalcoholic fatty liver disease in mice

Abstract

This study was to construct a nanovesicle delivery system to improve the loading efficiency and stability of ORI for the treatment of nonalcoholic fatty liver disease (NAFLD). This nanovesicles (NVs) exerted a narrow size distribution (195.6 ± 11.49 nm) and high entrapment efficiency (84.46 ± 1.34%). In vitro cell studies demonstrated that the NVs treatment enhanced the cellular uptake of ORI and reduced lipid over-accumulation and total cholesterol levels in NAFLD cell model. At the same time, in vivo study proved that, compared with the normal group, the model group mice showed a decrease in body weight, a significant increase in liver index (6.71 ± 0.62, p < 0.01), and symptoms of liver lipid accumulation, lipid vesicles, and liver tissue fibrosis. Compared with the model group, after high-dose ORI NVs intervention, mice gained weight, decreased liver index (4.69 ± 0.55, p < 0.01), reduced hepatic lipid droplet vacuoles, reduced lipid accumulation (reduced oil red area, p < 0.001), and alleviated the degree of liver fibrosis (reduced blue collagen area, p < 0.001). In conclusion, ORI/HP-β-CD/H9-HePC NVs showed specific liver accumulation and improved therapeutic effects, the nano drug loading system provides a promising strategy for the encapsulation of ORI to effectively alleviate the process of NAFLD.

Graphical Abstract

Keywords:

• Amphiphilic peptide
• lipid accumulation
• metabolic diseases
• oridonin

Acknowledgments

This work was financed by grant: This work was financed by grant: 23A350001 from key scientific research projects of colleges and universities in Henan Province. This work also was financed by grant: innovation and entrepreneurship training project for college students of Zhengzhou University.

Disclosure statement

The authors declare that there is no conflict of interest.

Data availability

Data will be made available on request.

Additional information

Funding

The author(s) reported there is no funding associated with the work featured in this article.