The Effect of the Ganglionic Segment Inflammatory Response to Postoperative Enterocolitis in Hirschsprung Disease

Abstract

Introduction

We examined the relationship between proinflammatory cytokines that occur in the inflammatory reaction in the intestine in Hirschsprung disease (HD) and Hirschsprung-associated enterocolitis (HAEC).

Methods

Thirty cases (M:27, F:3) operated on due to HD. The cases were divided into three groups: group 1 with pre and post operative EC, group 2 with post-operative, and group 3 with pre-operative EC. The intestinal segments were evaluated by immunohistochemistry for interleukin 1 beta (IL-1ß), tumor necrosis factor-alpha (TNF-α), and interleukin 6 (IL-6).

Results

IL-1β staining was significantly higher in the ganglionic zone of groups with enterocolitis compared to the control group (p = 0.012). TNF-α staining in the transitional zone of Group 3 and IL-1β staining in the ganglionic zone of Group 1 was significantly higher than the control group (p = 0.030, p = 0.020).

Conclusion

In our study, older age at diagnosis and more than 20% IL-1ß staining in the ganglionic segment were found to be risk factors for HAEC. It is noteworthy that the increase in IL-1ß can be associated with HAEC.

Keywords:

• Enterocolitis
• Hirschsprung’s disease
• inflammation
• IL-6
• IL-1β
• TNF-α

Acknowledgments

We thank Istanbul University-Cerrahpaşa Scientific Research Projects Unit for supporting our study (Project ID: 36569).

Thanks for the preprint (doi:10.21203/rs.3.rs-2666915/v1) available at Reseach Square.

Author contributions

Conceptualization: Yalım Benibol, Ayse Mine Onenerk, Rahsan Ozcan. Methodology: Nil Comunoglu, Ali Ekber Hakalmaz, Gonca Topuzlu Tekant, Yalım Benibol. Formal analysis and investigation: Yalım Benibol, Ayse Mine Onenerk, Rahsan Ozcan, Nil Comunoglu. Writing—original draft preparation: Yalım Benibol, Ayse Mine Onenerk, Rahsan Ozcan, Ali Ekber Hakalmaz. Writing—review and editing: Yalım Benibol, Nil Comunoglu, Gonca Topuzlu Tekant.

Disclosure statement

No potential conflict of interest was reported by the authors.

Funding

The study was supported by Istanbul University-Cerrahpaşa Scientific Research Projects Unit.