Determination of deoxynivalenol by ELISA and immunochromatographic strip assay based on monoclonal antibodies

Abstract

Deoxynivalenol (DON) has numerous toxicological effects. In this study, a monoclonal antibody (mAb) against DON with IC₅₀ 7.12 ng/mL was prepared, which was highly specific to trichothecene, except for 3-AC-DON. A quantitative indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and qualitative nanogold probe-based immunochromatographic strip (ICS) assay were established to detect DON. The analytical range of ic-ELISA was 0.90–83.07 ng/mL, and the limit of detection (LOD) were 0.20 ng/mL, 12.5 ng/mL for ic-ELISA and ICS, respectively. The results showed that the ic-ELISA and ICS based on the anti-DON mAb could be efficaciously applied to analyze agricultural products with DON.

Keywords:

• Deoxynivalenol
• monoclonal antibody
• indirect competitive enzyme-linked immunosorbent assay (ic-ELISA)
• immunochromatographic strip (ICS)

Author contributions

Peiwu Li and Qi Zhang conceived and designed the experiments; Tingting Yan performed the experiments; Tingting Yan, Du Wang and Xiaoqian Tang analyzed the data; Tingting Yan, Qi Zhang and Wen Zhang contributed reagents/materials/analysis tools; Tingting Yan wrote the paper.

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This work was supported by International Science & Technology Cooperation Program of China (2016YFE0119900), the Natural Science Foundation of Hubei Province (ZRMS2017001647) and The Major Project of Hubei Provincial Technical Innovation (2018ABA081).