Assessment of genotoxic and pathologic potentials of fipronil insecticide in Labeo rohita (Hamilton, 1822)

Abstract

Freshwater fish Labeo rohita were exposed to 0.03, 0.06, 0.09, 0.12 and 0.15 mg/L fipronil concentrations for 9 days in triplicate groups. Among signs loss of coordination, increased opercular movement, and tremors of fins were noted. Erythrocytic indices, lymphocytes, and monocytes decreased while total leukocyte counts, and neutrophils increased significantly. Relative weight of kidneys, gills, heart, and brain were significantly reduced. Gills and kidneys showed severe lesions. Erythrocytes showed a variety of nuclear abnormalities. Alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and lactate dehydrogenase (LDH) increased significantly in treated fish. It was concluded that fipronil induced severe clinical symptoms and adverse hemato-biochemical values in rohu fish, even at low concentrations.

Keywords:

• Fipronil
• fish
• hemato-biochemistry
• micronucleus assay
• genotoxicity

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

The authors acknowledge the Higher Education Commission, Islamabad (Pakistan) for providing the financial support to conduct the M.Phil thesis research, which was the basis for this article.