Abstract
Effect of Medicago sativa (MS) hydro-ethanolic extract against the cyclophosphamide (CP)-induced toxicity was evaluated in rats grouped as: Control; CP (100 mg/kg subcutaneous, day 1–3); and CP+ MS100, 200, and 400 mg/kg/day, orally for 14 days. Hematologic, lipid, hepatic and oxidative stress markers were analyzed. Platelet, WBC, RBC, Hb and Hct in the CP group were lower than the control. The MS200 and MS400 groups had lower lipids, liver enzymes, nitrite and malondialdehyde levels but higher platelet, WBC, thiol, superoxide dismutase and catalase levels than the CP group. MS ameliorated CP-induced thrombocytopenia, oxidative stress, and increases in serum lipids and hepatic enzymes.
Acknowledgements
The results mentioned in this article are part of an M.Sc. thesis.
Author contributions
Zahra gholamnezhad: designed and supervised the study, analyzed the data and prepared the manuscript. Vajihe Rouki: conducted the experiments and analyzed the data. Ramin Rezaee: advised the study and revised manuscript. Mohammad Hossein Boskabady: advised the study and revised manuscript. All authors approved the final version of the manuscript.
Disclosure statement
No potential conflict of interest was reported by the author(s).