Figures & data
Figure 1 Titration curves of ZIKV Equad in an (A) IgM and (B) IgG ELISA with ZIKV- positive and negative sera.
Figure 2 IgM ELISA on (A) 300 ng per well of DENV 1–4 Equad and (B) 200 ng per well of ZIKV Equad tested with IgM-positive DENV, ZIKV, WNV and flavivirus negative human sera. One sample per patient was examined in two independent experiments and plotted as a mean data point indicating absorbance values. The dotted lines represent cutoffs determined by a ROC analysis with negative sera as controls.
Table 1 Description of serum groups used for IgM measurements
Table 3 Sensitivity and specificity of DENV- and ZIKV- Equad in IgM- and IgG- measurements
Figure 3 IgG ELISA on (A) 160 ng per well of DENV 1–4 Equad and (B) 150 ng per well of ZIKV Equad measured with flavivirus positive sera, CHIKV and Malaria positive and negative sera. One sample per patient was examined in two independent experiments and plotted as a mean data point indicating absorbance values. The dashed lines represent cut-offs determined by a ROC analysis with negative sera as controls.
Table 2 Description of serum groups used for IgG measurements
Figure 4 Titration of the competing antigen (A) DENV Equad and (B) ZIKV Equad in Dengue and Zika positive sera and IgG measurement on the coated DENV Equad (blue lines) and ZIKV Equad (red lines).
Figure 5 IgG Competition ELISA: Sera were measured on (A) 160 ng/well of DENV 1–4 Equad with 200 ng per well ZIKV Equad competition and (B) 150 ng of ZIKV Equad with 2 μg of DENV 1–4 competition. One sample per patient was examined in two independent experiments and plotted as a mean data point. The dashed lines represent cutoffs determined by a ROC analysis with negative sera as controls.
Figure 6 IgG competition ELISA of paired serum samples from European travelers infected with ZIKV (A) and DENV (B) measured on 150 ng of ZIKV Equad (A) with DENV Equad competition and on 160 ng of DENV 1–4 Equad (B) with ZIKV competition.
