LC-MS/MS analysis of 11-nor-9-carboxy-hexahydrocannabinol (HHC-COOH) and 11-hydroxy-hexahydrocannabinol (HHC-OH) for verification of hexahydrocannabinol (HHC) intake

Figures & data

Figure 1. MS-chromatograms for co-eluting R/S-HHC-OH (top), R-HHC-COOH and S-HHC-COOH (mid), and THC-COOH-D9 internal standard (bottom).

Figure 2. MS-chromatogram for Δ8-THC-COOH (early peak) and Δ9-THC-COOH (late peak).

Table 1. Baseline characteristics of the studied samples.

Population characteristics
Age (years, mean;range)	32;16–50
Men:women	17;7
Samples tested	n
Total samples screened for THC	4152
Positive in immunoassay	512
Included in study	46
Pos. R/S-HHC-OH	38
Pos. R-HHC-COOH	32
Pos. S-HHC-COOH	6
Pos. Δ8-THC-COOH	5
Concentrations	Mean µg/L (range)
R-HHC-COOH	286 (10–2200)
S-HHC-COOH	23 (10–44)
R/S-HHC-OH	415 (10–2900)