HOTAIR knockdown impairs metastasis of cervical cancer cells by down-regulating metastasis-related genes

Figures & data

Table 1. List of small interfering RNA (siRNA) sequences used in the present study.

Genes	Sense（5'–3'）	Antisense（5'–3'）
HOTAIR siRNA1	CCTCCAGGCCCTGCCTTCTGCCTGCACATTCTGCCCTGAT	ATCAGGGCAGAATGTGCAGGCAGAAGGCAGGGCCTGGAGG
HOTAIR siRNA2	GGCAGGCAGTGGGGAACTCTGACTCGCCTGTGCTCTGGAGCTTG	CAAGCTCCAGAGCACAGGCGAGTCAGAGTTCCCCACTGCCTGCC
HOTAIR siRNA3	GGCAGGCAGTGGGGAACTCTGACTCGCCTGTGCTCTGGAGCTTGATCC	GGATCAAGCTCCAGAGCACAGGCGAGTCAGAGTTCCCCACTGCCTGCC
si-NC	GATCGATCTCCGAGTGTGTCACACAATTCAAGAGATTAC GTGACACGT	AATTGAATTAAATTCTCCGAACGTGTCACGTAATCTCTTGA ATTACGTGA

Table 2. List of primer sequences used in the present study.

Genes	Forward	Reverse
HOTAIR	AACCACGCAGAGAAATGCAG	CTCTCTGTACTCCCGTTCCC
Notch1	CTTTGTGCTTCTGTTCTTCGTG	CGCCGCTTCTTCTTGCT
EpCAM	TTCGGGCTTCTGCTTGC	CCCTTCAGGTTTTGCTCTTC
E-cadherin	ATCTGAAAGCGGCTGATACTG	TGCCCCATTCGTTCAAGTA
Vimentin	TCGTGATGCTGAGAAGTTTCG	TCTGGATTCACTCCCTCTGGT
STAT3	CTTTGAGACCGAGGTGTATCACC	GGTCAGCATGTTGTACCACAGG
GAPDH	CAATGACCCCTTCATTGACC	GAGAAGCTTCCCGTTCTCAG

Figure 1. HOTAIR levels and cell viability determined by OD value after DoTc2 and C-33-A cells were transfected with siHOTAIRs. (A) relative mRNA levels, (B) Cell viability. Experiments were repeated three times. Comparisons were made using one-way ANOVA. * and ** p < 0.05 and < 0.01.

Figure 2. Apoptosis, migration and invasion of DoTc2 and C-33-A cells after transfection using siHOTAIR1. (A) upper panel, flow cytometry results, lower panel: % of apoptosis cells. (Ba) left side: transwell assay results, right side: cell count. (Bb) migration assessment. Left side: transwell assay results, right side: cell count. Experiments were triplicated. Comparisons were made using one-way ANOVA. * and ** p < 0.05 and < 0.01.

Figure 3. Expression of invasions and metastasis-related genes in DoTc2 and C-33-A cells after HOTAIR knockdown. (A) relative mRNA levels, (B) left side: typical Western blot, right side; relative protein content. Experiments were triplicated. Comparisons were made using one-way ANOVA. * and ** p < 0.05 and < 0.01.