Figures & data
Figure 1. CSE inhibited the viability of NR8383 cells. The data was expressed as mean ± SEM (n=3); *P<0.05, **P<0.01, ***P<0.001 vs control group.
Figure 2. CSE induced autophagy impairment and inflammatory response in NR8383 cells. A: The ratio of LC3II/I protein, the level of P62 protein in NR8383 cells were detected by western blot. B: autophagosomes in NR8383 cells were detected by TEM (the yellow arrows indicated autophagosomes). C: Immunofluorence (IF) was applied to detect the fluorescence intensity of LAMP1 protein in NR8383 cells. Scale bar, 20µm. D: Lyso-Tracker Red staining was used to measure the acid environment of lysosomes in NR8383 cells; the intensity of red fluorescence was positively correlated with the acidic degree of lysosomes. Scale bar, 20µm. E: The levels of TNF-α, IL-1β, IL-6 were detected by ELISA kits. The data was expressed as mean ± SEM (n=3). *P<0.05, **P<0.01, ***P<0.001 vs. control group.
Figure 3. CSE prevented TFEB from entering into nucleus, which led to the increasing secretion of inflammatory cytokines in NR8383 cells. A: The ratio of LC3II/I protein, the level of P62 protein in NR8383 cells were detected by western blot. B: The levels of inflammatory cytokines TNF-α, IL-1β, IL-6 were detected by ELISA kits. C: The immunofluorence was used to analyze the TFEB location in NR8383 cells. Scale bar, 20µm. D: The expressions of TFEB protein in nucleus and cytosol were detected by western blot. E: Immunofluorence (IF) was used to detect the fluorescence intensity of LAMP1 protein in NR8383 cells. Scale bar, 20µm. F: Lyso-Tracker Red staining was used to measure the acid environment of lysosomes in NR8383 cells; the intensity of red fluorescence was positively correlated with the acidic degree of lysosomes. Scale bar, 20µm. The data was expressed as mean ± SEM (n=3). *P<0.05, **P<0.01, ***P<0.001 vs control group, #P<0.05, ##P<0.01, ###P<0.001 vs CSE group.
