Mismatch repair, p53 and chromosomal aberrations in primary colorectal carcinomas

Figures & data

Figure 1.  Karyogram depicting the chromosomal locations of all regions of DNA copy number imbalance in 49 sporadic colorectal carcinomas. Regions of deletion are represented by lines to the left of the chromosome ideograms and regions of gain by lines to the right. Thin black lines represent Group 1 tumours (p53 overexpression/MMR normal) shaded lines represent Group 2 tumours (p53 normal/MMR normal) and thick black lines represent Group 3 tumours (MMR defective/p53 normal).

Table I.  Clinicopathological and molecular data for the 49 primary colorectal tumours.

Tumour	Side*	Age	UICC Stage	p53 status	MMR status	No. of Gains	No. of Deletions	Total no. of Aberrations
1	Left	71	T3N0M0	Normal	Normal	0	0	0
2	Right	43	T4N0M1	Normal	hMSH2§	0	0	0
3	Right	55	T2N0M0	Normal	hMLH1	0	0	0
4	Left	76	T3N1M0	Defective	Normal	1	0	1
5	Left	77	T3N0M0	Defective	Normal	1	0	1
6	Right	72	T3N1M0	Normal	hMLH1	1	0	1
7	Right	63	T3N0M0	Normal	Normal	2	1	3
8	Right	73	T3N0M0	Defective	Normal	4	0	4
9	Right	51	T3N1M0	Normal	Normal	4	0	4
10	Left	63	T3N1M0	Normal	Normal	0	4	4
11	Left	87	T3N0M0	Normal	Normal	6	0	6
12	Left	62	T3N1M0	Defective	Normal	1	5	6
13	Right	81	T3N1M0	Normal	hMLH1	5	2	7
14	Left	75	T3N1M0	Normal	Normal	2	5	7
15	Left	71	T4N0M0	Defective	Normal	4	4	8
16	Left	61	T2N0M0	Normal	Normal	4	4	8
17	Right	75	T2N0M0	Normal	hMLH1	4	4	8
18	Left	52	T2N0M0	Normal	Normal	3	5	8
19	Left	59	T4N1M0	Defective	Normal	5	4	9
20	Right	73	T3N0M0	Normal	Normal	4	5	9
21	Left	70	T3N3M0	Defective	Normal	1	8	9
22	Left	79	T3N0M0	Defective	Normal	5	6	11
23	Right	68	T3N0M1	Normal	hMLH1	5	6	11
24	Right	71	T3N3M0	Normal	Normal	3	8	11
25	Left	76	T3N0M0	Defective	Normal	5	7	12
26	Right	64	T3N1M0	Defective	Normal	8	5	13
27	Right	52	T4N0M0	Normal	Normal	5	8	13
28	Right	61	T4N1M1	Defective	Normal	6	8	14
29	Left	76	T3N1M0	Normal	Normal	5	9	14
30	Left	61	T2N0M0	Normal	Normal	5	9	14
31	Left	67	T4N0M0	Normal	Normal	4	10	14
32	Left	62	T3N0M0	Defective	Normal	11	4	15
33	Left	67	T3N0M0	Defective	Normal	6	9	15
34	Left	76	T3N1M0	Defective	Normal	8	8	16
35	Right	77	T3N0M0	Normal	Normal	7	9	16
36	Left	68	T3N1M0	Normal	Normal	6	11	17
37	Left	65	T2N0M0	Normal	Normal	6	11	17
38	Left	62	T3N0M0	Defective	Normal	6	12	18
39	Left	70	T3N0M0	Defective	Normal	12	7	19
40	Left	74	T2N1M0	Defective	Normal	7	12	19
41	Left	44	T3N1M0	Normal	Normal	12	11	23
42	Right	42	T3N2M0	Defective	Normal	12	13	25
43	Left	69	T3N0M0	Defective	Normal	10	15	25
44	Right	63	T4N1M0	Normal	Normal	12	14	26
45	Left	73	T3N2M0	Defective	Normal	19	11	30
46	Right	71	T4N2M0	Normal	Normal	12	18	30
47	Right	81	T4N0M0	Defective	Normal	14	17	31
48	Left	74	T4N1M0	Defective	Normal	15	18	33
49	Left	86	T3N0M0	Defective	Normal	17	19	36

*Right and Left of splenic flexure. §Denotes loss of expression of (hMSH2 or hMLH1).

Table II.  Statistical analysis of median number of aberrations in colorectal tumours catagorised by p53 and MMR status.

	n	Number of Aberrations median (range)	p
Group by p53 status only
p53 overexpression	23	15 (1–36)
p53 normal	26	8.5 (0–30)	0.03†
Group by p53 & MMR status
GROUP 1 p53 overexpression/MMR normal	23	15 (1–36)
GROUP 2 p53 normal/MMR normal	20	12 (0–30)	0.17†
GROUP 3 p53 normal/MMR defective	6	4 (0–11)	<0.03 vs both groups†

†Mann-Whitney U test.