Expression of Bcl-2 Family Members in Malignant Pleural Mesothelioma

Figures & data

Table I.  Summary of Patient Details

Histological Subtype	No. Male	No. Female	Age Range (mean age)	Mean Survival Time
Epithelial	35	4	44–86 (67)	13 months
Sarcomatoid	13	2	50–94 (68)	5 months

Table II.  Details of antibody suppliers and dilutions used.

Antibody	Supplier & Catalogue No	Dilution
Bad	BD Biosciences (610391)	1:50
Bak	Neomarkers (RB-1250)	1:50
Bax	Neomarkers (MS-1335)	1:100
Bcl-2	Neomarkers (MS-123)	1:50
Bcl-XL	Neomarkers (MS-1334)	1:75
Bid	BD Biosciences (611528)	1:100
Bim	Neomarkers (RB-1612)	1:100
Mcl-1	Neomarkers (MS-681)	1:50
p53	BD Biosciences (554293)	1:100

Table III.  Details of Expression of p53 and Members of the Bcl-2 Family Proteins in Malignant Pleural Mesothelioma.

Protein		Overall n = 54	Epithelial MPM n = 39	Sarcomatoid MPM n = 15	Significance^a With Survival	Significance^b Between Histology
	p53	44/54 (81%)	33/39 (84%)	11/15 (73%)	ns	ns
Anti-apoptotic (overexpression)	Bcl-2	22/54 (40%)	17/39 (43%)	5/15 (33%)	ns	ns
	Bcl-XL	13/54 (24%)	6/39 (15%)	7/15 (46%)	ns	p = 0.03
	Mcl-1	50/54 (92%)	37/39 (94%)	13/15 (86%)	ns	ns
Pro-apoptotic (loss of expression)	Bad	14/54 (25%)	6/39 (15%)	8/15 (53%)	ns	p = 0.012
	Bak	13/54 (24%)	10/39 (25%)	3/15 (20%)	ns	ns
	Bax	23/54 (42%)	18/39 (46%)	5/15 (33%)	ns	ns
	Bid	20/54 (37%)	8/39 (20%)	12/15 (80%)	ns	p < 0.001
	Bim	10/54 (18%)	5/39 (12%)	5/15 (33%)	ns	ns

^aUnivariate Analysis: significance calculated with Log Rank analysis for results of 54 MPM cases (ns- not significant).

^bUnivariate Analysis: significance calculated with Fisher's Exact Test (2-sided) for results of epithelial against sarcomatoid cases.

Figure 1.  Kaplan Meier Survival Curve showing the survival patterns for sarcomatoid and epithelial MPM