p53 mutation and cyclin D1 amplification correlate with cisplatin sensitivity in xenografted human squamous cell carcinomas from head and neck

Figures & data

Table I.  Tumour origin and tumour line flow cytometry

Tumour line LU-HNxSCCX	Site of primary tumour origin	Ploidity	% s phase	DNA Indices
14	Oral cavity	Non-diploid	6.2	1.81
7	Bucca	Non-diploid	18.2	1.56
11	Primary tumour unknown	Non-diploid	16.6	1.33
12	Larynx	Non-diploid	4.8	1.31
5	Gingiva	Non-diploid	13.2	2.21
8	Bucca	Non-diploid	2.0	1.80
4	Oral cavity	Non-diploid	10.6	1.63

Table II.  Description of p53 mutation, cyclin D1 dysregulation and relative AUC in xenografts. The immunohistochemical results were scored: A negative; B 1–5% of the tumour cells positive; C 6–50% positive; D > 50% positive.

	p53 mut.	Amino acid	Type of mutation	IHC p53	FISH cycline D1	IHC cycline D1	Rel. AUC mean
LU-HNxSCX 14	-	-	-	A	-	B	0.19
LU-HNxSCX 7	-	-	-	A	-	B	0.29
LU-HNxSCX 11	Exon 6	Arg 196 ter Arg 72 Pro*	Nonsense	A	-	B	0.40
LU-HNxSCX 12	Exon 4	Ter 122	Frame shift	A	+	B	0.69
LU-HNxSCX 5	Exon 5 + 7	His 168 Pro 11e 254 Asn	Missence	D	+ + +	D	0.94
LU-HNxSCX 8	Exon 7	Leu 252 del	Inframe deletion	D	+1/3**	C	0.77
LU-HNxSCX 4	Exon 7	Arg 249 Gly	Missence	D	+	B	0.70

*contain also the R72P polymorphism.

**two cellpopulations were observed. One tumour cell population was FISH positive and represented 1/3 of all tumour cells in sample.

Figure 1.  Boxplot diagram of relative AUC in various tumour lines, showing the 2½, 25, 50, 75 and 97½ cumulative relative frequencies. (n = number of tumour lines).