Association of angiotensin-converting enzyme gene insertion/deletion polymorphism with increased risk for oral cancer

Figures & data

Figure 1.  Determination of ACE I/D polymorphism genotypes by PCR amplification. The presence of a 490 bp fragment (I allele) and a 190 bp fragment (D allele) indicate the possible genotypes DD, ID and II. M: molecular weight marker; 1, 2, 7 and 12: DD homozygotes; 3, 4, 6, 8, 10, 11 and 15: ID heterozygotes; 5, 9, 13 and 14: II homozygotes.

Table I.  Prevalence of ACE I allele and carrier frequencies in healthy controls and patients with oral cancer. Statistical difference of frequencies observed in patients and their subgroups in comparison to controls (p-values). N.S.: not significant p-value.

Prevalence of I allele	Controls	Patients	Patients With cancer stages I&II	Patients with cancer stages III&IV	Patients with positive family history of cancer	Patients without positive family history of cancer	Patients with positive family history of thrombosis	Patients without positive family history of thrombosis	Patients with tobacco abuse	Patients without tobacco abuse	Patients with alcohol abuse	Patients without alcohol abuse
I allele frequency	27.5%	40.6% (p < 0.001)	43.2% (p < 0.001)	37.5% (p < 0.05)	36.2% (p < 0.05)	43.1% (p < 0.001)	33.3% (p < 0.05)	42.3% (p < 0.001)	42.1% (p < 0.001)	12.5% (N.S.)	38.5% (p < 0.05)	33.3% (p < 0.001)
Carrier frequency of I allele	49%	62.5% (p < 0.05)	63.6% (p < 0.05)	61.1% (p < 0.05)	65.5% (p < 0.05)	60.8% (p < 0.05)	60% (N.S.)	63.1% (p < 0.05)	64.5% (p < 0.05)	25% (N.S.)	53.8% (N.S.)	66.7% (p < 0.05)