LRIG1 expression in colorectal cancer

Figures & data

Table I.  Clinical data of the 30 colorectal cancer patients in the study.

	n (%)
Age, years
Medium	70
Range	45–89
Sex
Female	19 (63)
Male	11 (37)
Stage
Dukes A	6 (20)
Dukes B	16 (53)
Dukes C	8 (27)
Site
Rectum and Sigmoid colon	13 (43)
Colon	17 (57)
Histological subtype/grade
Adenocarcinoma	27
Mucinous adenocarcinoma	3
Well differentiated	2
Moderately differentiated	23
Poorly differentiated	2
Deceased	16 (53)

Figure 1.  FISH analyses of LRIG1 locus in colorectal cancer cells from two patients. (A) Nuclei showing normal gene copy number (two copies per nucleus) of the LRIG1 gene. (B) Nuclei showing increased gene copy number (more than two copies per nucleus) of the LRIG1 gene at 3p14. Analysis was performed with a specific LRIG1 probe (red).

Figure 2.  Example of Western blot analyses of LRIG1 in samples from two colorectal cancer patients. For comparison, the samples were stepwise diluted by 50% in three steps. Tumours (T) versus matched non-neoplastic tissue (N) from the same patient. Pat 6 was considered underexpressing the LRIG1 protein in the tumour compared to adjacent non-neoplastic tissue and Pat 12 was considered as overexpressing the LRIG1 protein in the tumour compared to non-neoplastic tissue.

Table II.  Crosstable of LRIG1 Western blot (WB) results and immunohistochemical (IHC) EGFR scores in 30 colorectal cancer samples and matching adjacent non-neoplastic tissue.

	EGFR IHC Positive^a	EGFR IHC Negative	EGFR IHC Missing	Total
LRIG1 WB Downregulated#	8	1	4	13
LRIG1 WB Upregulated#	5*	4	4	13
LRIG1 WB Equal	0	2	2	4
Total	13	7	10	30

# = Tumour compared to normal tissue, where a visual change of at least 50% was considered significant.

=No visible change between tumour and normal tissue.

^a=Positive immunostaining in at least 10% of cells.

* = The tumour with LRIG1 increased copy number was one of the tumours in this group.

Missing = No paraffin embedded tissue available for IHC analysis.

Figure 3.  Immunohistochemical analyses of EGFR in primary colorectal tumors. (A) Negative staining in colorectal cancer cells and surrounding tissues. (B) Positive membranous staining in more than 10% of colorectal cancer cells (original magnification ×20), and mostly negative staining in surrounding tissues.