Aberrant expression of cyclin E in low-risk node negative breast cancer

Figures & data

Table I.  Patient characteristics and staining results.

	T	Elston Grade	PgR	Adjuvant		Follow-up	Cyclin A	Cyclin E	DB	Fraction DB
	(mm)			RT	ET	(months)	(%)	(%)	(%)	(%)
Node negative patients
Cases	13	I	−	yes	no	60	7	5	1.8	24
	18	II	+	no	no	66	11	5	0.5	4
	10	II	+	no	yes	32	9	1	0.3	4
	17	III	+	yes	no	41	25	6	3.2	13
	17	II	+	no	no	91	6	6	1.2	21
	12	III	−	yes	no	12	7	12	1.3	18
	15	I	−	no	no	16	14	0	0.0	0
	17	II	+	yes	no	44	7	8	1.4	21
	17	II	−	yes	yes	19	10	10	2.8	27
	10	III	−	yes	no	21	11	4	0.4	2
	12	II	+	yes	no	45	7	6	1.5	21
	17	III	+	yes	yes	20	10	2	0.9	9
	14	III	+	yes	no	16	9	4	0.9	10
	17	II	+	yes	no	67	35	7	5.1	15
	3	II	+	yes	no	99	1	1	0.0	0
	12	III	−	no	no	36	4	1	0.2	5
	12	II	+	no	no	47	6	3	0.5	8
Mean	15	II				43	11	5	1.3	12
Controls	15	I	+	no	no	152	25	4	3.3	14
	7	III	+	yes	no	136	2	4	0.2	10
	10	II	−	yes	no	145	20	8	6.3	31
	16	II	+	yes	no	148	4	3	0.1	3
	10	II	+	yes	no	158	13	1	0.5	4
	6	II	+	no	no	148	13	5	3.5	27
	16	II	+	yes	no	145	18	4	1.6	9
	14	III	+	yes	no	137	4	2	0.2	5
	19	II	−	yes	no	125	7	6	0.6	8
	16	III	+	yes	no	144	7	5	1.2	16
	9	I	+	yes	no	146	6	5	1.3	21
	6	III	+	yes	no	130	4	1	0.0	0
	14	II	+	yes	no	122	20	7	2.6	13
	5	I	+	yes	no	147	16	1	0.4	3
	17	II	+	no	no	133	6	6	0.8	13
	13	II	−	yes	no	123	12	1	0.4	3
	10	II	+	yes	no	132	20	2	0.7	3
	7	I	+	yes	no	138	3	2	0.1	5
	10	I	+	no	no	122	6	4	0.3	5
	11	II	+	yes	no	133	12	4	1.6	14
	13	I	+	yes	no	149	4	4	1.5	38
	12	II	+	yes	no	133	21	7	5.3	25
	11	I	+	yes	no	133	18	12	7.6	42
	10	II	+	yes	no	159	30	11	7.9	26
Mean	12	II				139	12	5	2.0	14

All tumours are T1, N0 and stain positive for oestrogen and/or progesteron.

T = tumor size, PgR = progesteron receptors, += positive, −= negative, RT = radiotherapy, ET = endocrine treatment, Follow-up = survival in cases, DB = doublestained, fraction DB = fraction doublestained cells in S-phase.

Figure 1.  Diagrams showing percent of all tumour cells staining positive for cyclin E and aberrant expression of cyclin E, i.e. fraction of cells co-expressing cyclins A and E, in cases and controls with node negative breast cancer.

Figure 2.  Photos illustrating a. cell nuclei stained with DAPI, b. cyclin A positive cells (Cy 5), c. cyclin E positive cells (Cy 3) and d. cells co-expressing cyclins A and E (arrows).