Increased perfusion pressure enhances the expression of endothelin (ET_B) and angiotensin II (AT₁, AT₂) receptors in rat mesenteric artery smooth muscle cells

Figures & data

Figure 1. ET_B and AT₂ mRNA expression from rat mesenteric artery. No perfusion pressure (C), normal perfusion pressure (NP) and high perfusion pressure (HP) for 4 h. *p<0.05.

Figure 2. (a) ET_B mRNA expression from rat mesenteric artery perfused with Actinomycin D. Organ culture (OC), normal perfusion pressure (NP) and high perfusion pressure (HP) for 2 and 4 h of incubation. (b) AT₂ mRNA expression from rat mesenteric artery perfused with Actinomycin D. Organ culture (OC), normal perfusion pressure (NP) and high perfusion pressure (HP) for 2 and 4 h of incubation.

Figure 3. ET_B protein expression from rat mesenteric artery. Normal perfusion pressure (NP) and high perfusion pressure (HP) for 1, 3 and 17 h. *p<0.05.

Figure 4. (a) AT₁ protein expression from rat mesenteric artery. Normal perfusion pressure (NP) and high perfusion pressure (HP) for 1, 3 and 17 h. *p<0.05. (b) AT₂ protein expression from rat mesenteric artery. Normal perfusion pressure (NP) and high perfusion pressure (HP) for 1, 3 and 17 h. *p<0.05.

Figure 5. (a) Immunohistochemistry. Left top: AT₁ protein expression during normal perfusion pressure (NP) and 1 h. Right top: AT₁ protein expression during NP and 17 h. Left bottom: AT₁ protein expression during high perfusion pressure (HP) and 1 h. Right bottom: AT₁ protein expression during HP and 17 h. (b) Immunohistochemistry. Left top: AT₂ protein expression during NP and 1 h. Right top: AT₂ protein expression during NP and 17 h. Left bottom: AT₂ protein expression during HP and 1 h. Right bottom: AT₂ protein expression during HP and 17 h. (c) Immunohistochemistry. Left top: ET_B protein expression during NP and 1 h. Right top: ET_B protein expression during NP and 17 h. Left bottom: ET_B protein expression during HP and 1 h. Right bottom: ET_B protein expression during HP and 17 h.

Figure 6. Co‐localization. First column shows protein receptor distribution for the receptors. Second column shows alpha‐actin distribution. Third column shows columns 1 and 2 merged together depicting co‐localization.