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Laboratory Studies

Determination of Ghrelin Immunoreactivity in Kidney Tissues of Diabetic Rats

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Pages 562-566 | Received 15 Apr 2009, Accepted 18 May 2009, Published online: 09 Sep 2009

Figures & data

Figure 1. Control group kidney sections. (a) Renal cortex, (b) medulla, and (c) papilla. Moderate (++) ghrelin immunoreactivity in the distal tubular epithelium, containing macula densa regions (a). Moderate (++) ghrelin immunoreactivity in the distal tubules (b). No positive staining in the glomeruli, proximal tubules, thin limbs of loop of Henle, and collecting ducts (a–c). Abbreviations: MD = Macula densa, G = glomeruli, PT = proximal tubule, DT = distal tubule, (→) = thin limbs of loop of Henle (☆) collecting duct. Magnification: ×10.

Figure 1. Control group kidney sections. (a) Renal cortex, (b) medulla, and (c) papilla. Moderate (++) ghrelin immunoreactivity in the distal tubular epithelium, containing macula densa regions (a). Moderate (++) ghrelin immunoreactivity in the distal tubules (b). No positive staining in the glomeruli, proximal tubules, thin limbs of loop of Henle, and collecting ducts (a–c). Abbreviations: MD = Macula densa, G = glomeruli, PT = proximal tubule, DT = distal tubule, (→) = thin limbs of loop of Henle (☆) collecting duct. Magnification: ×10.

Figure 2. Diabetes2 group kidney sections. (a) Renal cortex, (b) medulla, and (c) papilla. Moderate (++) ghrelin immunoreactivity in the distal tubules (a) and (b). No positive staining in the glomeruli, proximal tubules, or thin limbs of loop of Henle (a, b). Low (+) ghrelin immunoreactivity in the collecting ducts in the renal papilla (c). Abbreviations: G = glomeruli, PT = proximal tubule, DT = distal tubule, (→) = thin limbs of loop of Henle (☆) collecting duct. Magnification: ×10.

Figure 2. Diabetes2 group kidney sections. (a) Renal cortex, (b) medulla, and (c) papilla. Moderate (++) ghrelin immunoreactivity in the distal tubules (a) and (b). No positive staining in the glomeruli, proximal tubules, or thin limbs of loop of Henle (a, b). Low (+) ghrelin immunoreactivity in the collecting ducts in the renal papilla (c). Abbreviations: G = glomeruli, PT = proximal tubule, DT = distal tubule, (→) = thin limbs of loop of Henle (☆) collecting duct. Magnification: ×10.

Figure 3. Diabetes4 group kidney sections. (a) Renal cortex, (b) medulla, and (c) papilla. Strong (+++) ghrelin immunoreactivity in the distal tubules (a) and (b). No positive staining in the glomeruli, proximal tubules, or thin limbs of loop of Henle (a, b). Moderate (++) ghrelin immunoreactivity in the collecting ducts in the renal papilla (c). Abbreviations: G = glomeruli, PT = proximal tubule, DT = distal tubule, (→) = thin limbs of loop of Henle (☆) collecting duct. Magnification: ×10.

Figure 3. Diabetes4 group kidney sections. (a) Renal cortex, (b) medulla, and (c) papilla. Strong (+++) ghrelin immunoreactivity in the distal tubules (a) and (b). No positive staining in the glomeruli, proximal tubules, or thin limbs of loop of Henle (a, b). Moderate (++) ghrelin immunoreactivity in the collecting ducts in the renal papilla (c). Abbreviations: G = glomeruli, PT = proximal tubule, DT = distal tubule, (→) = thin limbs of loop of Henle (☆) collecting duct. Magnification: ×10.

Figure 4. Diabetes6 group kidney sections. (a) Renal cortex, (b) medulla, and (c) papilla. Strong (+++) ghrelin immunoreactivity in the distal tubules (a) and (b). No positive staining in the glomeruli, proximal tubules, or thin limbs of loop of Henle (a, b). Strong (+++) ghrelin immunoreactivity in the collecting ducts in the renal papilla (c). Abbreviations: G = glomeruli, PT = proximal tubule, DT = distal tubule, (→) = thin limbs of loop of Henle (☆) collecting duct. Magnification: ×10.

Figure 4. Diabetes6 group kidney sections. (a) Renal cortex, (b) medulla, and (c) papilla. Strong (+++) ghrelin immunoreactivity in the distal tubules (a) and (b). No positive staining in the glomeruli, proximal tubules, or thin limbs of loop of Henle (a, b). Strong (+++) ghrelin immunoreactivity in the collecting ducts in the renal papilla (c). Abbreviations: G = glomeruli, PT = proximal tubule, DT = distal tubule, (→) = thin limbs of loop of Henle (☆) collecting duct. Magnification: ×10.

Figure 5. (a) Positive control. Ghrelin immunoreactive cells in stomach. (→). (b) Negative control section in renal cortex. Magnification: ×10.

Figure 5. (a) Positive control. Ghrelin immunoreactive cells in stomach. (→). (b) Negative control section in renal cortex. Magnification: ×10.

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