A Mendelian randomization study: physical activities and chronic kidney disease

Figures & data

Figure 1. Scatter plots for MR analysis of physical activities on CKD for exploring casual effects. a. Heavy DIY b. Light DIY c. Strenuous sports d. Walking for pleasure e. Other exercises.

Figure 2. Scatter plots for MR analysis of physical activities on eGFR for exploring casual effects. a. Heavy DIY b. Light DIY c. Strenuous sports d. Walking for pleasure e. Other exercises.

Table 1. MR analysis results from several methods for exploring casual effect of different physical activities on kidney function(CKD and eGFR).

Exposures	No.of SNP	R^2	F-statistics	IVW		WMM		MR-Egger
				OR/ß (95%CI)	p value	OR/ß (95%CI)	p value	OR/ß (95%CI)	p value
CKD as Outcome(OR)
Heavy DIY	18	0.0000178	35.0	0.287 0.117 − 0.705	0.0065	0.395 0.12 8 − 1.221	0.107	0.376 0.001 − 133.495	0.748
Light DIY	13	0.0000217	40.2	0.679 0.201 − 2.125	0.479	0.679 0.185 − 2.498	0.560	0.859 0.006 − 116.904	0.953
Strenuous sports	6	0.0000077	38.3	1.683 0.152 − 18.649	0.67	4.201 0.326 − 54.20	0.27	0.010 3.77e-08 − 2464.254	0.505
Walking for Pleasure	20	0.0000151	34.1	0.603 0.271 − 1.339	0.214	0.636 0.222 − 1.823	0.40	0.316 6.157e-06 − 16262.497	0.838
Other exercises	14	0.000021	41.2	1.018 0.439 − 2.361	0.967	1.084 0.372 − 3.157816	0.883	25.481 0.0430 − 15100.210	0.340
eGFR as Outcome(ß)
Heavy DIY	18	0.0000178	35.0	0.036 0.005 − 0.067	0.021	0.029 −0.008 − 0.066	0.129	0.085 −0.116 − 0.286	0.420
Light DIY	13	0.0000217	40.2	0.006 −0.075 − 0.087	0.890	0.01 −0.045 − 0.065	0.714	−0.072 −0.405 − 0.261	0.680
Strenuous sports	6	0.0000077	38.3	0.012 −0.128 − 0.152	0.867	−0.014 −0.109 − 0.081	0.773	0.398 −0.242 − 1.038	0.290
Walking for Pleasure	20	0.0000151	34.1	0.035 −0.013 − 0.082	0.154	0.053 0.011 − 0.095	0.013	0.207 −0.437 − 0.851	0.536
Other exercises	14	0.000021	41.2	−0.001 −0.044 − 0.042	0.960	−0.022 −0.065 − 0.022	0.332	−0.384 −0.644 – (-0.123)	0.014

IVW: inverse variance weighting, WMM: weighed median method, LOO: ‘leave -one- out’ sensitivity analysis, Heavy DIY types of physical activity in last 4 weeks: Heavy DIY (e.g.,: weeding, lawn mowing, carpentry, digging), Light DIY types of physical activity in last 4 weeks: Light DIY (e.g.,: pruning, watering the lawn), Strenuous sports types of physical activity in last 4 weeks: Strenuous sports, Walking for pleasure types of physical activity in last 4 weeks: Walking for pleasure (not as a means of transport), Other exercises types of physical activity in last 4 weeks: Other exercises (e.g.,: swimming, cycling, keep fit, bowling).

Significants of bold values means statistically significant (p < 0.05). 

Table 2. MR analysis results from several methods for exploring casual effect of kidney function(CKD and eGFR) on different physical activities.

Outcome	No.of SNP	R2	F-statistics	IVW		WMM		MR-Egger
				ß (95%CI)	p value	ß (95%CI)	p value	ß (95%CI)	p value
CKD as Exposures
Heavy DIY	21	0.0028	64.7	−0.0003 −0.0061 − 0.0054	0.912	0.004 −0.0046 − 0.012	0.367	0.002 −0.013 − 0.017	0.787
Light DIY	21	0.0028	64.7	4.724e-04 −0.0054 − 0.0063	0.874	3.49e-03 −0.005 − 0.012	0.42	1.918e-05 −0.0145 − 0.0146	0.998
Strenuous sports	21	0.0028	64.7	−9.54e-06 −0.0036 − 0.0035	0.996	2.861e-04 −0.0053 − 0.0059	0.921	4.779e-04 −0.0083 − 0.0093	0.917
Walking for Pleasure	21	0.0028	64.7	0.0021 −0.0041 − 0.0084	0.503	−0.0021 −0.0102 − 0.0059	0.603	−0.0045 −0.0201 − 0.011	0.578
Other exercises	21	0.0028	64.7	−1.3e-03 −0.0088 − 0.0062	0.733	−8.78e-05 −0.0093 − 0.0091	0.985	−1.44e-04 −0.019 − 0.019	0.988
eGFR as Exposures
Heavy DIY	197	0.0000046	76.2	−0.037 −0.099 − 0.025	0.237	−0.041 −0.116 − 0.035	0.292	−0.115 −0.266 − 0.035	0.135
Light DIY	197	0.0000046	76.2	0.00076 −0.072 − 0.073	0.984	−0.081 −0.16 − 0.0016	0.046	−0.069 −0.245 − 0.108	0.448
Strenuous sports	197	0.0000046	76.2	−0.0058 −0.044 − 0.032	0.767	0.0152 −0.033 − 0.063	0.534	0.0005 −0.093 − 0.094	0.992
Walking for Pleasure	197	0.0000046	76.2	0.0208 −0.043 − 0.085	0.525	0.023 −0.051 − 0.097	0.545	−0.076 −0.232 − 0.080	0.339
Other exercises	197	0.0000046	76.2	0.029 −0.039 − 0.097	0.4	−0.033 −0.116 − 0.0497	0.434	−0.075 −0.24 − 0.091	0.377

IVW: inverse variance weighting; WMM: weighed median method; LOO: ‘leave -one- out’ sensitivity analysis; Heavy DIY types of physical activity in last 4 weeks: Heavy DIY (e.g., weeding, lawn mowing, carpentry, digging), Light DIY types of physical activity in last 4 weeks: Light DIY (e.g., pruning, watering the lawn), Strenuous sports types of physical activity in last 4 weeks: Strenuous sports, Walking for pleasure types of physical activity in last 4 weeks: Walking for pleasure (not as a means of transport), Other exercises types of physical activity in last 4 weeks: Other exercises (e.g., swimming, cycling, keep fit, bowling).